中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2141-2144.doi: 10.3969/j.issn.1673-8225.2012.12.012

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

脱细胞真皮基质补片修复滇南小耳猪胆管损伤☆

陈  刚，白建华，刘其雨，李晓延，朱新锋，王  谦，赵英鹏，李镜锋，李  立   

  1. 昆明医学院附属甘美医院肝胆外科，云南省昆明市  650034
  • 收稿日期:2011-12-07 修回日期:2012-02-22 出版日期:2012-03-18 发布日期:2012-03-18
  • 通讯作者: 李立，博士，教授，昆明市第一人民医院肝胆外科，云南省昆明市，650034 ynkmlil@ yahoo.com.cn
  • 作者简介:陈刚☆，男，1969年生，四川省南充市人，汉族，1994年川北医学院毕业，博士，副主任医师，主要从事器官移植方面研究。 kmcg123@ 163.com

Acellular dermal matrix for the repair of bile duct injury in Diannan small-ear pigs

Chen Gang, Bai Jian-hua, Liu Qi-yu, Li Xiao-yan, Zhu Xin-feng, Wang Qian, Zhao Ying-peng, Li Jing-feng, Li Li   

  1. Department of Hepatobiliary Surgery, the First People’s Hospital of Kunming (the Affiliated Ganmei Hospital of Kunming Medical College), Kunming  650034, Yunnan Province, China
  • Received:2011-12-07 Revised:2012-02-22 Online:2012-03-18 Published:2012-03-18
  • Contact: author: Li Li, Ph.D., Professor, Department of Hepatobiliary Surgery, the First People’s Hospital of Kunming (the Affiliated Ganmei Hospital of Kunming Medical College), Kunming 650034, Yunnan Province, China ynkmlil@ yahoo.com.cn
  • About author:Chen Gang☆, Ph.D., Associate chief physician, Department of Hepatobiliary Surgery, the First People’s Hospital of Kunming (the Affiliated Ganmei Hospital of Kunming Medical College), Kunming 650034, Yunnan Province, China kmcg123@163.com

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摘要：

背景：目前胆管缺损的修复效果不佳，脱细胞真皮基质已广泛用于烧伤、疝修复、口腔黏膜及软组织修复等。
目的：观察脱细胞真皮基质修复滇南小耳猪胆管损伤的效果。
方法：将滇南小耳猪建立胆管损伤动物模型，建模成功后按修复方式分为胆肠吻合组、膨体聚四氟乙烯组及脱细胞真皮基质组，胆肠吻合组进行胆肠吻合修补，膨体聚四氟乙烯组及脱细胞真皮基质组分别用膨体聚四氟乙烯补片和脱细胞真皮基质补片制成直径稍宽管状进行修补。
结果与结论：治疗后2~4个月，脱细胞真皮基质组的总胆红素低于其他2组(P < 0.05)；免疫组织化学染色显示，治疗后1~4个月，其转化生长因子β1表达均高于其他2组(P < 0.05)，表达随时间增加而降低(P < 0.05)。脱细胞真皮基质组胆总管吻合口及周围组织出现胆道上皮、腺体、血管及平滑肌等再生。脱细胞真皮基质组治疗后未出现胆道狭窄、感染及排斥反应发生。结果证实，脱细胞真皮基质可生理性修复胆道并重建其功能，并发症少，生物相容性较好。
关健词：脱细胞真皮基质；补片；胆管损伤；转化生长因子β1；猪；生物相容性；细胞外基质材料
doi:10.3969/j.issn.1673-8225.2012.12.012

关键词: 脱细胞真皮基质, 补片, 胆管损伤, 转化生长因子&beta, 1, 猪, 生物相容性, 细胞外基质材料

Abstract:

BACKGROUND: Acellular dermal matrix is available for burn, hernia repair, breast reconstruction, soft tissue reconstruction, etc. Research of repair for bile duct injury with acellular dermal matrix has not been previously reported in the recent literature.
OBJECTIVE: To observe acellular dermal matrix effect on the repair of bile duct injury in Diannan small-ear pigs.
METHODS: Bile duct injury models were established in 72 Diannan small-ear pigs which were randomly divided into three groups and treated with biliary intestinal anastomosis, expanded polytetrafluorethylene and acellular dermal matrix, respectively.
RESULTS AND CONCLUSION: Total bilirubin level in the acellular dermal matrix group was lower than that in the other two groups at 2-4 months after treatment (P < 0.05). The expression of transforming growth factor β1 in the acellular dermal matrix group was higher as compared with the other two groups at 1-4 months after treatment (P < 0.05), and it decreased along with time passing. Regeneration of biliary epithelial cells, gland, vascular endothelial cells and smooth muscle, even nerve was detected in the acellular dermal matrix group under light microscope and electron microscope. No similar detection occurred in other groups. biliary stricture, infection and rejection were not found in the acellular dermal matrix group. These findings suggest that acellular dermal matrix can be used to physiologically repair bile duct defects with less complications and good biocompatibility.

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