中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2145-2148.doi: 10.3969/j.issn.1673-8225.2012.12.013

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

近红外照射下金纳米链及Anti-EGFR/Au共轭物对人喉癌Hep-2细胞的抑制*☆

丛林海，彭淑昆，何晓光，杨一兵   

  1. 昆明医学院第一附属医院耳鼻咽喉科，云南省昆明市  650031
  • 收稿日期:2011-11-19 修回日期:2011-12-31 出版日期:2012-03-18 发布日期:2012-03-18
  • 通讯作者: 何晓光，博士，教授，昆明医学院第一附属医院耳鼻咽喉科，云南省昆明市 650031 hexg1018@ yahoo.com.cn
  • 作者简介:丛林海☆，男，1972年生，黑龙江省伊春市人，汉族，2011年昆明医学院毕业，博士，主治医师，主要从事头颈肿瘤基础及临床研究。conglinhai2008@126.com 并列第一作者：彭淑昆，女，1965年生，云南省昆明市人，汉族，1986年昆明医学院毕业，主管技师。
  • 基金资助:

    国家自然科学基金项目(81160324/H1625)，课题名称: EGFR-mAb功能化修饰金纳米棒光热诱导Hep-2细胞凋亡分子机制研究。

Apoptosis of human laryngeal cancer cells induced by gold nanochain and anti-epidermal growth factor receptor/Au conjugate under near-infrared hyperthermia 

Cong Lin-hai, Peng Shu-kun, He Xiao-guang, Yang Yi-bing   

  1. Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming  650031, Yunnan Province, China
  • Received:2011-11-19 Revised:2011-12-31 Online:2012-03-18 Published:2012-03-18
  • Contact: author: He Xiao-guang, Doctor, Professor, Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China hexg1018@ yahoo.com.cn
  • About author:Cong Lin-hai☆, Doctor, Attending physician, Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China conglinhai2008@ 126.com Peng Shu-kun, Technician In charge, Department of Otolaryngology, the First Affiliated Hospital of Kunming Medical College, Kunming 650031, Yunnan Province, China
  • Supported by:

     the National Natural Science Foundation of China, No. 81160324/H1625*

PDF

334

摘要：

背景：金纳米颗粒在近红外激光照射下对肿瘤细胞具有杀伤效应。
目的：评价金纳米链及anti-EGFR/Au共轭物近红外照射对人喉癌Hep-2细胞的生长抑制和热杀伤作用。
方法：取对数生长期的Hep-2细胞，随机分为空白对照组、单纯照射组、金纳米链照射组和anti-EGFR/Au照射组，加入不同培养液后，各照射组进行近红外激光照射。观察照射后细胞凋亡情况和凋亡程度。
结果与结论：倒置显微镜下观察显示空白对照组和单纯照射组Hep-2细胞仍具有活性，金纳米链照射组有明显的损伤，但anti-EGFR/Au照射组损伤程度更重。流式细胞分析显示金纳米链照射组及anti-EGFR/Au靶向照射组均有不同程度的Hep-2细胞凋亡，且照射时间越长，凋亡细胞越多。说明金纳米链近红外照射可以杀伤人喉鳞癌Hep-2细胞，anti-EGFR/Au靶向照射比金纳米链照射效果更好，并呈现一定照射时间的依赖性。
关键词：金纳米链；Hep-2细胞；流式细胞分析；照射；生物材料与纳米技术
doi:10.3969/j.issn.1673-8225.2012.12.013

关键词: 金纳米链, Hep-2细胞, 流式细胞分析, 照射, 生物材料与纳米技术

Abstract:

BACKGROUND: The gold nanoparticles have a killing effect on tumor cells under the near-infrared laser irradiation.
OBJECTIVE: To evaluate the growth inhibition and heat killing effect of gold nanochain and anti-epidermal growth factor receptor (EGFR)/Au conjugate on human laryngeal cancer Hep-2 cells under near-infrared hyperthermia.
METHODS: The Hep-2 cells in the logarithmic growth phase were collected and divided into blank control group, irradiation group, gold nanochain irradiation group and anti-EGFR/Au irradiation group. After added with different culture medium, the near-infrared laser irradiation was performed in each group. Then the apoptosis of Hep-2 cells in different experimental groups was observed after the near-infrared laser irradiation.
RESULTS AND CONCLUSION: Inverted microscope observation showed that the Hep-2 cells in the blank control and irradiation groups were still active and the Hep-2 cells in the gold nanochain irradiation group were damaged significantly, while the Hep-2 cells in anti-EGFR/Au irradiation group were damaged more serious. Flow cytometry analysis showed that the Hep-2 cells in the gold nanochain irradiation and anti-EGFR/Au irradiation groups had a varying degree of apoptosis and the increasingly heavy damage with the time. The gold nanochain irradiation can kill Hep-2 cells and the anti-EGFR/Au irradiation has a better effect in a time-depended manner.

中图分类号: