中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (16): 2884-2888.doi: 10.3969/j.issn.1673-8225.2012.16.008

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

磁性碳纳米管载附5-氟尿嘧啶对结肠癌淋巴结转移的靶向抑制作用☆

金彦召，谭  敏   

  1. 中山大学附属第一医院微创外科，广东省广州市 510080
  • 收稿日期:2011-09-09 修回日期:2011-10-03 出版日期:2012-04-15 发布日期:2012-04-15
  • 通讯作者: 谭敏，教授，博士生导师，中山大学附属第一医院微创外科，广东省广州市 510080 tantommy@ 163.com
  • 作者简介:金彦召☆，男，1981年生，河南省漯河市人，汉族，中山大学附属第一医院在读博士，医师，主要从事外科微创手术学研究。 jyz998152@ 126.com

Target inhibitory effect of magnetic carbon nanotubes loaded with 5-fluorouracil on lymph node metastasis of colon cancer 

Jin Yan-zhao, Tan Min   

  1. Department of Minimal Invasive Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2011-09-09 Revised:2011-10-03 Online:2012-04-15 Published:2012-04-15
  • Contact: author: Tan Min, Professor, Doctoral supervisor, Department of Minimal Invasive Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China tantommy@163.com
  • About author:Jin Yan-zhao☆, Studying for doctorate, Physician, Department of Minimal Invasive Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China jyz998152@126.com

PDF

287

被引次数

5

摘要：

背景：将碳纳米管制备成磁靶向药物载体后，在磁场作用下能更好地把药物运送至体内靶器官或靶组织。
目的：观察磁性碳纳米管新化疗载体对结肠癌淋巴结转移的抑制效果。
方法：MTT法检测5-氟尿嘧啶、磁性多壁碳纳米管-5-氟尿嘧啶、磁性多壁碳纳米管(每种药物分别含0.000 3，0.003，0.03，0.3，3 g/L 5-氟尿嘧啶)对结肠癌SW480细胞的抑制作用。将含相同浓度5-氟尿嘧啶的5-氟尿嘧啶、磁性多壁碳纳米管-5-氟尿嘧啶、磁性多壁碳纳米管分散液分别注入SD大鼠足垫皮下及结肠癌淋巴结转移裸鼠体内。
结果与结论：体外各质量浓度的5-氟尿嘧啶和磁性多壁碳纳米管-5-氟尿嘧啶对癌细胞的毒性存在剂量依赖性，相同5-氟尿嘧啶质量浓度时两者对体外SW480细胞的抑制作用无明显差异，说明磁性多壁碳纳米管-5-氟尿嘧啶的主要药效成分为5-氟尿嘧啶。体内研究高效液相检测显示磁性多壁碳纳米管-5-氟尿嘧啶能有效聚集在淋巴结，长时间持久释放，淋巴结浓集效果明显优于5-氟尿嘧啶(P < 0.05)，且不良反应小，肉眼容易辨识，细胞穿透性好；TUNNEL检测见磁性多壁碳纳米管-5-氟尿嘧啶化疗后结肠癌淋巴结转移灶细胞有明显凋亡现象，在磁场作用下效果更显著。说明磁性多壁碳纳米管-5-氟尿嘧啶对结肠癌SW480细胞淋巴结转移有明显抑制作用。
关键词：磁性碳纳米管；5-氟尿嘧啶；淋巴化疗；结肠癌；淋巴结转移；生物材料与纳米技术
缩略语注释：5-FU：5-fluorouracil，5-氟尿嘧啶；mMWNTs：multi walled carbon nanotubes，磁性多壁碳纳米管
doi:10.3969/j.issn.1673-8225.2012.16.008
 

关键词: 磁性碳纳米管, 5-氟尿嘧啶, 淋巴化疗, 结肠癌, 淋巴结转移, 生物材料与纳米技术

Abstract:

BACKGROUND: Carbon nanotubes are prepared by magnetic targeting drug carrier in the magnetic field to better drug delivery to target organs or tissues in vivo.
OBJECTIVE: To evaluate the inhibitory effect of the magnetic carbon nanotubes as a new chemotherapy carrier on lymph node metastasis of colon cancer.
METHODS: The inhibitory effects of different concentrations of 5-fluorouracil (5-FU), magnetic multi-walled carbon nanotubes-5-fluorouracil (mMWNTs-5-FU) and magnetic multi-walled carbon nanotubes (mMWNTs) (0.000 3, 0.003, 0.03, 0.3,   3 g/L 5-FU) on the growth of SW480 cells in vitro were measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. 5-FU, mMWNTs-5-FU and mMWNTs were injected into subcutaneous footpad of Sprague-Dawley rats and lymph node metastasis of colon cancer nude mice.
RESULTS AND CONCLUSION: Treatment of the cancer cells with various concentrations (0.000 3, 0.003, 0.03, 0.3, 3 g/L 5-FU) of mMWNTs-5-FU caused a dose-dependent decrease in cell viability relative to the control culture, with the same 5-FU levels. The in vitro inhibition of SW480 cells had no significant difference, showing the main ingredient of mMWNTs-5-FU is 5-FU. High pressure liquid chromatography detection in vivo showed that mMWNTs-5-FU could effectively gather in the lymph nodes and prolong release. Lymph concentration was better than 5-FU (P < 0.05), and adverse reactions were less, visually easy to identify, cell-penetrating good; terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method detected clear apoptosis of lymph node metastasis cells after the mMWNTs-5-FU chemotherapy. This shows colon cancer lymph node metastasis is significantly inhibited by mMWNTs-5-FU chemotherapy.

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