中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2133-2136.doi: 10.3969/j.issn.1673-8225.2012.12.010

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

铁碳纳米粒介导顺铂对喉癌细胞敏感性的研究*

朱  力1，侯  楠2，胡国华1   

  1. 1重庆医科大学附属第一医院耳鼻喉科，重庆市  400016；2成都医学院第一附属医院耳鼻喉科，四川省成都市  610500
  • 收稿日期:2011-11-30 修回日期:2012-01-14 出版日期:2012-03-18 发布日期:2012-03-18
  • 通讯作者: 胡国华，博士，主任医师，教授，重庆医科大学附属第一医院耳鼻喉科，重庆市 400016 myhcsh@ 163.com
  • 作者简介:朱力，女，1963年生，湖南省张家界市人，汉族，主任医师，主要从事耳鼻咽喉疾病的临床治疗及基础研究。 574307370@ qq.com
  • 基金资助:

    四川省卫生厅立项项目(100107)，课题名称：铁碳纳米粒介导顺铂对喉癌细胞的敏感性影响研究。

Effect of carbon coated iron nanoparticles carrying diamminedichloroplatinum on the chemotherapy sensitivity of laryngocarcinoma cells 

Zhu Li1, Hou Nan2, Hu Guo-hua1   

  1. 1Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China; 2Department of Otorhinolaryngology, the First Affiliated Hospital of Chengdu Medical College, Chengdu  610500, Sichuan Province, China
  • Received:2011-11-30 Revised:2012-01-14 Online:2012-03-18 Published:2012-03-18
  • Contact: author: Hu Guo-hua, Doctor, Chief physician, Professor, Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China myhcsh@163.com
  • About author:Zhu Li, Chief physician, Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China 574307370@qq.com
  • Supported by:

    Sichuan Provincial Health Department Project, No. 100107*

PDF

317

被引次数

3

摘要：

背景：应用纳米技术对化疗药物进行靶向性改进是增加肿瘤对化疗药物敏感性的有效手段之一。
目的：观察铁碳纳米粒搭载顺铂对喉癌Hep-2细胞的抑制作用及对细胞Caspase 3，Survivin mRNA表达的影响。
方法：分别应用铁碳纳米粒和(或)顺铂的生理盐水分散液干预喉癌Hep-2细胞，以生理盐水干预的细胞作为对照。
结果与结论：MTT检测结果显示，顺铂可明显抑制Hep-2细胞的生长，与铁碳纳米粒联合应用对Hep-2细胞的抑制作用更强；表现为细胞生长不贴壁，增殖缓慢，出现凋亡征象。RT-PCR结果显示，共培养5 d，铁碳纳米粒和顺铂联合及顺铂单独处理的Hep-2细胞Caspase 3 mRNA水平明显增高，而Survivin mRNA水平明显降低，以铁碳纳米粒和顺铂联合作用效果更明显，而单独应用铁碳纳米粒对Hep-2细胞无影响。提示铁碳纳米粒搭载顺铂能够增加喉癌Hep-2细胞对顺铂的敏感性，提高药物化疗效果。
关键词：铁碳纳米粒；顺铂；喉癌；Hep-2细胞；化疗；敏感性；Caspase3；Survivin
doi:10.3969/j.issn.1673-8225.2012.12.010

关键词: 铁碳纳米粒, 顺铂, 喉癌, Hep-2细胞, 化疗, 敏感性, Caspase3, Survivin

Abstract:

BACKGROUND: Nanometer technique is one of the effective ways to improve chemotherapy sensitivity of tumor cells by refining dosage form and target action of chemotherapy.
OBJECTIVE: To investigate the depression effect of carbon coated iron nanoparticles carrying diamminedichloroplatinum (CCINs-DDP) on Hep-2 laryngocarcinoma cells and expression of caspase 3 and survivin mRNA.
METHODS: Hep-2 laryngocarcinoma cells were intervened by iron-carbon nanoparticles and (or) normal saline dispersion of cisplatin. Cells interfered with normal saline were selected as controls.
RESULTS AND CONCLUSION: Methylthiazolyldiphenyl-tetrazolium bromide assay showed that cisplatin could inhibit cell growth of Hep-2 cells, and combined with the iron-carbon nanoparticles, the inhibitory effect was stronger. Cells were not adherent, appeared with slow proliferation and apoptosis. Reverse transcription-PCR results showed that caspase 3 mRNA level of the Hep-2 cells was significantly increased at co-cultured 5 days after treated with iron-carbon nanoparticles combined with cisplatin and simple treated with the cisplatin, but the expression of survivin mRNA was significantly decreased, which was more obviously after treated with iron-carbon nanoparticles combined with cisplatin, but simple application of iron-carbon nanoparticles had no effect on Hep-2 cells.
It is indicated that CCINs-DDP can improve the chemotherapy sensitivity of Hep-2 laryngocarcinoma cells on the DDP and also improve therapeutic efficacy of chemotherapeutics.
 

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