中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (28): 4523-4528.doi: 10.3969/j.issn.2095-4344.2017.28.016

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

脑海马神经元接受化疗与未化疗的差异

李建平1，2，卢 巍1，杨 琳1，谢密新3，贺 旭4，潘爱华2   

  1. 1遵义医学院珠海校区人体解剖学教研室，广东省珠海市 519041；2中南大学湘雅医学院人体解剖与神经生物学系，湖南省长沙市 410013；3益阳医学高等专科学校人体解剖学教研室，湖南省益阳市 413000；4武汉大学医学职业技术学院，湖北省武汉市 430060
  • 修回日期:2017-07-26 出版日期:2017-10-08 发布日期:2017-11-10
  • 通讯作者: 潘爱华，博士，教授，中南大学湘雅医学院人体解剖与神经生物学系，湖南省长沙市 410013
  • 作者简介:李建平，男，1980年生，汉族，湖南省岳阳市人，2015年中南大学毕业，硕士，讲师，主要从事神经生物方向的研究。
  • 基金资助:

    国家自然科学基金(61650305)；遵义医学院硕士启动基金(F-77)

Neurons in the hippocampus of chemobrain versus non-chemotherapy brain

Li Jian-ping1, 2, Lu Wei1, Yang Lin1, Xie Mi-xin3, He Xu4, Pan Ai-hua2   

  1. 1Department of Anatomy, Zunyi Campus of Zunyi Medical University, Zhuhai 519041, Guangdong Province, China; 2Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha 410013, Hunan Province, China; 3Department of Anatomy, Yiyang Medical College, Yiyang 413000, Hunan Province, China; 4Medicine Vocational and Technical School of Wuhan University, Wuhan 430060, Hubei Province, China
  • Revised:2017-07-26 Online:2017-10-08 Published:2017-11-10
  • Contact: Pan Ai-hua, M.D., Professor, Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha 410013, Hunan Province, China
  • About author:Li Jian-ping, Master, Lecturer, Department of Anatomy, Zunyi Campus of Zunyi Medical University, Zhuhai 519041, Guangdong Province, China; Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha 410013, Hunan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 61650305; the Master Start-Up Foundation of Zunyi Medical University, No. F-77

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183

被引次数

3

摘要：

文章快速阅读：

文题释义：
大脑海马区hippocampus：是帮助人类处理长期学习与记忆声、光、味觉等事件的大脑区域，发挥所谓的叙述性记忆declarative memory功能。在医学上海马区是大脑皮质的一个内褶区，在侧脑室底部绕脉络膜裂形成一弓形隆起，它由2个扇形部分所组成，有时将两者合称海马结构。
化疗：是化学药物治疗的简称，是利用化学药物阻止癌细胞的增殖、浸润、转移，直至最终杀灭癌细胞的一种治疗方式。它是一种全身性治疗手段，和手术、放疗一起，并称为癌症的3大治疗手段。由于化疗药物的选择性不强，在杀灭癌细胞的同时也会不可避免地损伤人体正常的细胞，从而出现药物的不良反应。因此，在接受化疗药物的时候，一方面希望能够达到最佳的抗肿瘤作用，另一方面也要注意预防和识别化疗药物的不良反应。

 

摘要
背景：研究认为化疗药物能穿过血脑屏障引起神经毒性作用而损伤神经细胞。
目的：观察化疗脑海马神经元的表达情况及形态的变化，探讨化疗脑对海马神经再生的影响的机制。  
方法：收集恶性肿瘤化疗人脑标本(< 60岁，n=3；> 60岁，n=3)和未化疗人脑标本(< 60岁，n=3；> 60岁，n=3)，免疫组织化学检测海马CA1、CA2和CA3区特异抗体阳性神经元的形态及数量的变化情况。
结果与结论：①不同年龄组免疫组织化学染色结果显示，与未化疗脑相比，化疗脑海马CA1、CA2和CA3区：NeuN+细胞、DCX+细胞、PV+细胞阳性数量明显降低，并且PV+细胞胞体较小，突起减少并缩短；细胞表达明显减少；②免疫荧光染色结果显示，< 60岁化疗脑NeuN阳性神经元和DCX阳性神经元共表达数量显著较未化疗脑低，而> 60岁化疗脑与未化疗脑无明显差异；③结果表明，药物化疗使海马未成熟神经元的表达降低；药物化疗使海马成熟神经元的数量降低；药物化疗使PV神经元形态发生改变，细胞数量降低；药物化疗降低海马未成熟神经元向成熟神经元的转化。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0003-2844-6699(李建平)

关键词: 组织构建, 组织工程, 化疗脑, 海马, DCX, PV, NeuN, 国家自然科学基金

Abstract:

BACKGROUND: Chemotherapy drugs have been shown to penetrate the blood-brain barrier and cause neurotoxicity, further damaging nerve cells.
OBEJCTIVE: To investigate the changes in the expression and morphology of hippocampal neurons in chemobrain, and to investigate the underlying mechanism of nerve regeneration in chemobrain.
METHODS: The brain specimens were obtained from the six patients undergoing cancer chemotherapy and six patients without chemotherapy, and were then subdivided into two groups: < 60 years and > 60 years groups (n=3 per group). Immunofluorescence staining with specific antibodies was used to detect the changes in the morphology and number of neurons in the hippocampus CA1, CA2 and CA3.
RESULTS AND CONCLUSION: The number of neurons positive for NeuN, DCX and PV in the hippocampal CA1, CA2 and CA3 in the chemotherapy group was significantly lower than that in the non-chemotherapy group. Moreover, the neurons positive for PV were smaller, with less and shorter nervous processes. Immunofluorescence staining findings showed that the < 60 years old patients in the chemotherapy group had less neurons positive for NeuN and DCX than that in the non-chemotherapy; for > 60 years old patients, there was no significant difference between two groups. These findings suggest that chemotherapy can downregulate the expression of immature neurons in the hippocampus and reduce the number of neurons in the hippocampus. Moreover, chemotherapy can change the morphology and reduce the number of PV neurons.


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Drug Therapy, Hippocampus, Neurons, Neoplasms, Tissue Engineering

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