中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1866-1870.doi: 10.3969/j.issn.1673-8225.2012.10.034

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

微小RNA-155在小鼠骨髓来源M1和M2巨噬细胞中表达的差异**★

梁艳冰，王中华，唐  皓，马中富   

  1. 中山大学附属第一医院普内科，广东省广州市 510080
  • 收稿日期:2012-01-08 修回日期:2012-02-04 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 马中富，教授，博士生导师，中山大学附属第一医院普内科，广东省广州市 510080 mazhongfu05@sohu.com
  • 作者简介:梁艳冰★，女，1968年生，广东省佛山市人，汉族，硕士，副主任医师，硕士生导师，主要从事脓毒血症基础和临床研究。 xuyw8591@126.com
  • 基金资助:

    广东省科技计划项目(2009B080701070；2009B080701004)，课题名称：miRNA-155在调控脓毒症小鼠SOCS1通路中作用的研究；IL-4/IL-13活化的M2巨噬细胞对脓毒症的免疫调节机制。

Diverse expression patterns of microRNA-155 between bone marrow-derived M1 and M2 macrophages  

Liang Yan-bing, Wang Zhong-hua, Tang Hao, Ma Zhong-fu   

  1. Department of General Internal Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2012-01-08 Revised:2012-02-04 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Ma Zhong-fu, Professor, Doctoral supervisor, Department of General Internal Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China mazhongfu05@sohu.com
  • About author:Liang Yan-bing★, Master, Associate chief physician, Master’s supervisor, Department of General Internal Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China xuyw8591@126.com
  • Supported by:

     the Science and Technology Development Program of Guangdong Province, No. 2009B080701070*, 2009B080701004*

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摘要：

背景：目前对微小RNA-155在巨噬细胞中的表达和功能的研究还集中在总体单核巨噬细胞水平。
目的：了解小鼠骨髓来源M1和M2巨噬细胞中微小RNA-155表达的差异。
方法：用100 U/mL干扰素γ和5 μg/L脂多糖诱导骨髓细胞来源的巨噬细胞向M1分化，用10 μg/L白细胞介素4诱导出M2巨噬细胞。
结果与结论：流式细胞检测显示实验诱导的M1和M2巨噬细胞纯度分别达91%和95%。RT-PCR检测显示诱导型一氧化氮合酶mRNA在M1巨噬细胞中高表达，在M2中则基本不表达；而Ⅰ型精氨酸酶、发现于炎症区域1 mRNA在M2中高表达，在M1中则表达较低；炎症因子肿瘤坏死因子α mRNA在M1中的表达明显高于M2，相反白细胞介素10 mRNA在M2中的表达高于M1(P < 0.05)。实时荧光定量PCR检测显示M1巨噬细胞中微小RNA-155的表达量明显高于M2(P < 0.05)。提示微小RNA-155可作为鉴别M1，M2巨噬细胞的标志。

关键词: 微小RNA-155, 骨髓细胞, M1巨噬细胞, M2巨噬细胞, 小鼠, 基因表达

Abstract:

BACKGROUND: Previous studies of microRNA-155 expression and function in macrophage predominantly focus on total mononuclear macrophage.
OBJCTIVE: To evaluate the expression difference of microRNA-155 in M1 and M2 macrophages.
METHODS: Bone marrow-derived macrophages were induced to differentiate towards M1 and M2 macrophages using interferon-γ (100 U/mL) + lipopolysaccharide (5 ng/mL) and interleukin-4 (10 ng/mL), respectively.
RESULTS AND CONCLUSION: The differentiation proportion of M1 and M2 macrophages detected by flow cytometry was 91% and 95%, respectively. Reverse transcription PCR method showed that inducible nitric oxide synthase (iNOS) mRNA was highly expressed in M1 macrophages, but it was hardly expressed in M2 macrophages. Arginase-1 and found in inflammatory zone 1 mRNA expression was highly expressed in M2 macrophages, but the expression was low in M1 macrophages. Tumor necrosis factor α mRNA expression was significantly higher in M1 macrophages than in M2 macrophages, on the contrary, interleukin 10 mRNA expression was significantly higher in M2 macrophages than in M1 macrophages (P < 0.05). Real-time fluorescence quantitative PCR showed that microRNA-155 expression in M1 macrophages was significantly higher compared with that in M2 macrophages (P < 0.05). These findings suggest that MiRNA-155 can serve as a useful maker for differential diagnosis of M1 and M2 macrophages.

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