中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1856-1860.doi: 10.3969/j.issn.1673-8225.2012.10.032

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

异种脐血干细胞移植胶原性关节炎小鼠Bcl-2和Bax的表达*☆

牛广华，高玉洁，孙  旭，都  静，明彩荣，高明利   

  1. 辽宁中医药大学附属医院临床检验中心，辽宁省沈阳市  110032
  • 收稿日期:2011-11-10 修回日期:2012-01-07 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 高玉洁，主任技师，辽宁中医药大学附属医院临床检验中心，辽宁省沈阳市 110032
  • 作者简介:牛广华☆，男，1972年生，辽宁省锦州市人，满族， 2010年辽宁中医药大学毕业，博士，副主任技师，主要从事自身免疫性疾病研究。
  • 基金资助:

    辽宁省自然基金项目资助(20092033)。

Bcl-2 and Bax expression in mice with collagen-induced arthritis following heterogenic umbilical cord blood stem cell transplantation*☆

Niu Guang-hua, Gao Yu-jie, Sun Xu, Du Jing, Ming Cai-rong, Gao Ming-li   

  1. Clinical Inspection Center, Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang  110032, Liaoning Province, China
  • Received:2011-11-10 Revised:2012-01-07 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Gao Yu-jie, Chief technician, Clinical Inspection Center, Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China jie_54@sohu.com
  • About author:Niu Guang-hua☆, M.D., Associate chief technician, Clinical Inspection Center, Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China ngh@263.net
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No. 20092033*

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摘要：

背景：类风湿性关节炎的发生发展与滑膜细胞及淋巴细胞的增殖与凋亡不平衡密切相关，其滑膜细胞存在细胞凋亡过程的异常。
目的：观察异种、异基因双份脐血干细胞移植对Ⅱ型胶原性关节炎小鼠Bcl-2和Bax表达的影响。
方法：弗氏完全佐剂+Ⅱ型胶原诱导C57BL/6(H-2b)小鼠，建立Ⅱ型胶原性关节炎小鼠模型。小鼠二次免疫接种后第2天，模型组、正常对照组尾静脉注射生理盐水，细胞移植组将脐血造血干细胞注入小鼠尾静脉内(其中单份剂量2×106/50 g；双份剂量：每份1×106/50 g，共2×106/50 g)。甲氨喋呤阳性对照组小鼠灌胃甲氨蝶呤，每次0.017 5 g/kg，每5天1次，共6次。
结果与结论：移植后第42天全部处死动物取膝及肘以下关节组织，病理组织学显示正常对照组关节面光滑，滑膜层未见炎性细胞浸润，软骨细胞形态正常；模型组滑膜组织高度增生，大量的炎性细胞浸润，软骨面破坏；甲氨蝶呤阳性对照组、单份脐血造血干细胞移植组滑膜组织可见轻度增生，少量炎性细胞浸润，双份脐血造血干细胞移植组关节软骨表面光滑，未见破坏，有极少量炎性细胞浸润。免疫组织化学检测显示，双份脐血造血干细胞移植组Bax、Bcl-2的表达低于单份脐血造血干细胞移植治疗组(P < 0.05)；与正常对照组比较，差异无显著性意义(P > 0.05)。结果说明双份脐血干细胞在一定数量及作用时间内可诱导Ⅱ型胶原性关节炎小鼠滑膜细胞凋亡，对滑膜组织损伤起保护作用。

关键词: 类风湿关节炎, 脐血造血干细胞, 细胞凋亡, Bax, Bcl-2

Abstract:

BACKGROUND: The occurrence and development of rheumatoid arthritis were strongly associated with unbalance proliferation and apoptosis of synovial cells and lymphocytes. Some synovial cell apoptosis was abnormal.
OBJECTIVE: To observe effects of heterogenic double umbilical cord blood stem cell transplantation on Bcl-2 and Bax expression in mice with type Ⅱ collagen-induced arthritis.
METHODS: C57BL/6(H-2b) mice were induced with Freund’s complete adjuvant and type Ⅱ collagen to establish mouse models of type Ⅱ collagen-induced arthritis. At 2 days after secondary immunity incubation, mice were injected with saline via tail vein in the model and normal control groups. Umbilical cord blood hemopoietic stem cells were injected into mouse tail vein in the UBSCs transplantation groups (single dose: 2×106/50 g; double dose: 1×106/50 g each, totally 2×106/50 g). Mice were intragastrically administrated methotrexate in the methotrexate positive control group, 0.017 5 g/kg once, once every 5 days, totally six times.
RESULTS AND CONCLUSION: Joint tissue below knee and elbow was obtained at 42 days following transplantation. Histopathology displayed that smooth and glossy articular surface, no inflammatory cell infiltration in the synovial layer and normal chondrocytes in the normal control group. Hyperplasia, a lot of inflammatory cell infiltration and damaged cartilage surface were visible in the model group. Slight hyperplasia and a little inflammatory cell infiltration were detectable in the methotrexate positive control group and single UBSCs transplantation group. Double UBSCs transplantation group exhibited smooth and glossy articular cartilage surface, no damage, a little inflammatory cell infiltration. Immunohistochemistry demonstrated that Bcl-2 and Bax expression was lower in the double UBSCs transplantation group compared with single UBSCs transplantation group (P < 0.05). Compared with normal control group, no significant difference was detected (P > 0.05). Results suggest that double umbilical cord blood stem cells can induce synovial cell apoptosis in mice with type Ⅱ collagen-induced arthritis in a certain number and action time, and protect synovial membrane against damage.

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