中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1245-1250.doi: 10.3969/j.issn.1673-8225.2012.07.025

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

深静脉血栓形成模型大鼠股静脉内皮组织中核转录因子kappa B1和组织因子的表达******☆

李兴国1，郑宏宇1，李  文2，李宏昆1，赵学凌1，吴雪梅1，王  兵1   

  1. 1昆明医学院第一附属医院骨科，云南省昆明市  650032；2云南省人民医院心内科，云南省昆明市  650032
  • 收稿日期:2011-11-12 修回日期:2011-12-12 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 王兵，博士，副主任医师，昆明医学院第一附属医院骨科，云南省昆明市 650032 并列通讯作者：吴雪梅，主管护师，昆明医学院第一附属医院骨科，云南省昆明市 650032
  • 作者简介:李兴国☆，男，1981年生，云南省楚雄市人，汉族，昆明医学院在读博士，讲师，主要从事深静脉血栓形成分子机制的研究。 并列第一作者：郑宏宇，男，1978年生，云南省大理市人，汉族，昆明医学院在读博士，医师，主要从事深静脉血栓预测诊断的研究。
  • 基金资助:

    基金声明：国家自然科学基金资助项目(30960389，81060151，81160236)；云南省科技厅-昆明医学院联合项目(2009cd159)；云南省重点新产品项目开发计划项目(2010BC010)；昆明医学院博士研究生创新基金资助项目(2011D07)。

Expression of nuclear factor kappa B1 and tissue factor in femoral venous endothelial tissue of the rat deep vein thrombosis model******☆

Li Xing-guo1 , Zheng Hong-yu1, Li Wen2, Li Hong-kun1, Zhao Xue-ling1, Wu Xue-mei1, Wang Bing1   

  1. 1Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China; 2Department of Cardiology, Yunnan Provincial People’s Hospital, Kunming  650032, Yunnan Province, China
  • Received:2011-11-12 Revised:2011-12-12 Online:2012-02-12 Published:2012-02-12
  • Contact: Wang Bing, Doctor, Associate chief physician, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China wangbingdoctor@126.com Wu Xue-mei, Nurse-in-charge, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China ynwuxuemei@126.com
  • About author:Li Xing-guo,☆ Studying for doctorate, Lecturer, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China ynwuxuemei@126.com Zheng Hong-yu, Studying for doctorate, Physician, Department of Orthopedics, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30960389*, 81060151*, 81160236*; Joint Funds of Yunnan Sci-Tech Department and Kunming Medical College, No. 2009cd159*; Yunnan New Key Product Development Projects, No. 2010BC010*; Doctor Innovation Foundation of Kunming Medical College, No. 2011D07*

PDF

461

被引次数

3

摘要：

背景：目前调控静脉内皮细胞、血小板、炎性细胞之间相互作用，促进局部深静脉血栓微环境形成的机制尚不完全清楚，仍未发现早期诊断深静脉血栓的可靠方法。
目的：研究核转录因子kappa B1和组织因子在大鼠深静脉血栓形成中的作用。
方法：将67只SD大鼠随机分为对照组和模型组，对模型组采用股静脉钳夹联合下肢石膏制动构建大鼠深静脉血栓形成模型。于造模后2.5，25 h，解剖股静脉观测血栓的发生情况，进一步将模型组分为：血栓形成前组(造模后2.5 h)、血栓形成组和血栓不形成组(造模后25 h)。取各组股静脉内皮组织，采用基因芯片筛查差异表达的基因，并进一步采用real-time PCR进行验证。
结果与结论：基因芯片分析及real-time PCR结果均发现，造模后2.5 h，血栓形成前组大鼠股静脉内皮组织中核转录因子kappa B1和组织因子表达明显高于对照组(P < 0.05)；造模后25 h，血栓形成组大鼠股静脉内皮组织中核转录因子kappa B1和组织因子表达明显高于血栓形成前组、血栓不形成组和对照组(P < 0.05)。提示局部静脉内皮组织中核转录因子kappa B1和组织因子表达水平上调可能在深静脉血栓形成中发挥了重要作用。

关键词: 核转录因子kappa B1, 组织因子, 静脉内皮组织, 深静脉血栓

Abstract:

BACKGROUND: At present, the basic underlying molecular mechanism regulating the interactions among venous endothelial cells, platelets, leukocytes, and promoting local deep vein thrombosis microenvironment formation, still remains unclear, and there is no ideal method for early diagnosis of deep vein thrombosis.
OBJECTIVE: To study the underlying role of nuclear factor kappa B1 and tissue factor in rats with deep vein thrombosis.
METHODS: A total of 67 Sprague-Dawley rats were randomly divided into control group (n=10) and model group (n=57). Deep vein thrombosis model was established by a clamping and sewing method in femoral vein combined with cast fixation. The incidence and serious degree of thrombus were observed by dissecting rat femoral vein in different time points (2.5 and 25 hours after modeling). The model group was further divided into pre-thrombogenesis group (2.5 hours after modeling), thrombogenesis group (25 hours after modeling) and non-thrombogenesis group (25 hours after modeling). Then total RNA was extracted from the localized femoral venous endothelial tissue. The candidate genes, associated inflammation and thrombosis, were screened by a special gene chip. Then the gene expression of nuclear factor kappa B1 and tissue factor was further identified by real-time polymerase chain reaction.
RESULTS AND CONCLUSION: Pre-thrombogenesis group had no thrombogenesis, while thrombogenesis group have 23 cases with thrombosis and non-thrombogenesis group have 22 cases without thrombosis. The results of gene chip hybridization analysis and real-time PCR found that the mRNA expression of nuclear factor kappa B1 and tissue factor in rat femoral vein endothelial tissue were significantly up-regulated at 2.5 hours after modeling (pre-thrombogenesis group was higher than control group) (P < 0.05), and continued up-regulating at 25 hours after modeling (thrombogenesis group was higher than the pre-thrombogenesis group, non-thrombogenesis group and control group) (P < 0.05). The results from present study indicate that up-regulating expressions of nuclear factor kappa B1 and tissue factor in local femoral venous endothelial tissue of rat deep vein thrombosis models may play a key role in initiating venous thrombosis.

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