中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1197-1200.doi: 10.3969/j.issn.1673-8225.2012.07.014

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

聚焦超声对羊肝脾组织的损伤效应★

韩艳艳，王  刚，李  芳，谢  军   

  1. 乌鲁木齐市眼耳鼻喉专科医院耳鼻喉科，新疆维吾尔自治区乌鲁木齐市  830002
  • 收稿日期:2011-07-12 修回日期:2011-10-18 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 王刚，硕士，主任医师，乌鲁木齐市眼耳鼻喉专科医院耳鼻喉科，新疆维吾尔自治区乌鲁木齐市 830002 entwanggang@163.com
  • 作者简介:韩艳艳★，女，1978年生，新疆维吾尔自治区乌鲁木齐市人，汉族，2011年新疆医科大学毕业，硕士，主治医师，主要从事耳鼻咽喉科临床研究。405038500@qq.com

Damage effect of focused ultrasound on sheep liver and spleen

Han Yan-yan, Wang Gang, Li Fang, Xie Jun   

  1. Department of Otorhinolaryngology, Urumqi Eyes-Ear- Nose-Throat Hospital, Urumqi  830002, Xinjiang Uygur Autonomous Region, China
  • Received:2011-07-12 Revised:2011-10-18 Online:2012-02-12 Published:2012-02-12
  • Contact: Wang Gang, Master, Chief physician, Department of Otorhinolaryngology, Urumqi Eyes- Ear- Nose-Throat Hospital, Urumqi 830002, Xinjiang Uygur Autonomous Region, China entwanggang@163.com
  • About author:Han Yan-yan★, Master, Attending physician, Department of Otorhinolaryngology, Urumqi Eyes- Ear- Nose-Throat Hospital, Urumqi 830002, Xinjiang Uygur Autonomous Region, China 405038500@qq.com

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摘要：

背景：高强度聚焦超声可引起组织损伤。
目的：研究聚焦超声对羊肝脏和羊脾脏组织的损伤效应。
方法：用不同强度和不同方法的聚焦超声波辐照5只羊的体内肝脏、体外肝脏及体外脾脏，观察其辐照后大体及病理学改变。
结果与结论：聚焦超声辐照体外羊肝的即刻组织损伤反应较辐射治疗1周后的体内羊肝重；体内羊肝及体外羊肝在不同治疗强度下“＃”法的组织损伤反应较“=”法重；不同强度及不同方法辐照体外羊脾后均未见明显组织反应；聚焦超声Ⅲ档“＃”法辐照羊肝组织可见多个灶性的肝细胞变性及凝固性坏死；聚焦超声Ⅳ档“＃”法辐照体内羊肝组织可见被膜下炎性纤维肉芽肿性包裹结节，中心大片凝固性坏死；聚焦超声Ⅳ档“＃”法辐照体外羊肝组织可见被膜下大部分区域肝小叶结构零散不清，肝窦明显扩大，肝细胞索明显变窄，凌乱不清。说明聚焦超声Ⅲ档“＃”法能够按照设计要求选择性的损伤羊肝脏组织。

关键词: 关键词：聚焦超声, 高强度, 肝脏, 脾脏, 病理学, 组织损伤

Abstract:

BACKGROUND: High-intensity focused ultrasound can induce tissue damages.
OBJECTIVE: To study the damage effect of focused ultrasound on the tissues of sheep liver and spleen.
METHODS: Focused ultrasounds with different intensities and different methods were used to radiate the livers in vivo, livers and spleens in vitro of five sheep. Visual and pathological changes were observed after radiation.
RESULTS AND CONCLUSION: The immediate damage of tissues of livers in vitro with focused ultrasound was more severe than the livers in vivo after 1 week of radiation therapy; in therapies with different intensities, the tissue damage of “#” method was more severe than “=” method in both in vivo and in vitro sheep livers; no significant damage was observed in sheep spleens in vitro with radiation therapies with different intensities and methods; focused ultrasound radiation of III file and “#” method resulted in a great number of hepatic cells with focal degeneration and coagulative necrosis; focused ultrasound radiation of IV file and “#” method on in vivo sheep liver tissues resulted in inflammatory fibrous granulomatous nodules under envelope and coagulative necrosis in large central areas; focused ultrasound radiation of IV file and “#” method on sheep liver tissues in vitro resulted in scattered hepatic lobule structures in most regions under envelope, significantly expanded hepatic sinus and significantly narrower hepatic cords, messy and unclear. Focused ultrasound with III file and “#” methods can damage sheep hepatic cells selectively according to design requirements, and provided experimental basis of treatment of allergic rhinitis.

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