中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 1107-1110.doi: 10.3969/j.issn.1673-8225.2012.06.034

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

骨髓间充质干细胞标记及示踪技术的研究与进展★

孙江森，李  彪，龚跃昆   

  1. 昆明医学院第一附属医院骨科，云南省昆明市 650023
  • 收稿日期:2011-06-28 修回日期:2011-08-08 出版日期:2012-02-05 发布日期:2012-02-05
  • 通讯作者: 龚跃昆，博士，主任医师，昆明医学院第一附属医院骨科，云南省昆明市 650023
  • 作者简介:孙江森★，男，1983年生，山东省诸城市人，汉族，昆明医学院在读硕士。jiangsen1983@163.com

Research progress in labeling and tracing technique of bone marrow mesenchymal stem cells 

Sun Jiang-sen, Li Biao, Gong Yue-kun   

  1. Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming  650023, Yunnan Province, China
  • Received:2011-06-28 Revised:2011-08-08 Online:2012-02-05 Published:2012-02-05
  • Contact: Gong Yue-kun, Doctor, Chief physician, Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650023, Yunnan Province, China
  • About author:Sun Jiang-sen★, Studying for master’s degree, Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650023, Yunnan Province, China jiangsen1983@163.com

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摘要：

背景：骨髓间充质干细胞标记及示踪技术是干细胞移植治疗的研究热点之一。虽然近几年骨髓间充质干细胞标记及示踪技术有了很大进展，但仍存在很多问题有待进一步解决。
目的：对国内外骨髓间充质干细胞标记及示踪技术的研究与进展作一综述。
方法：应用计算机检索CNKI和Foreign Medical Journal Service数据库中1982-01/2011-10关于骨髓间充质干细胞标记及示踪技术的文章，在标题和摘要中以“骨髓间充质干细胞；标记方法；示踪技术”或“bone marrow，labeling，Tracer”为检索词进行检索。最终选择29篇文献进行综述。
结果与结论：骨髓间充质干细胞的示踪技术众多，主要有同位素示踪法、抗原标记法、荧光蛋白标记法、荧光染料标记法、核磁共振对比增强剂标记法、Lac-Z基因标记法、Y染色体标记法。每一种方法都有其优缺点，选择的标记方法应具有特异性强、灵敏度高、对细胞或机体影响小、标记和检测方法简单易行、标记时间合适等特点。种子细胞的示踪技术仍需要进一步深入研究。

关键词: 标记方法, 骨髓间充质干细胞, 示踪技术, 综述, 文献

Abstract:

BACKGROUND: Cytological markers and tracer technology of bone marrow mesenchymal stem cells (BMSCs) are the focus in transplantation treatment of stem cells. Although in recent years, cytological markers and tracer technology of BMSCs have made great progress, there are still many problems need to be solved.
OBJECTIVE: To review the research and development of tracer technique and cytological markers of BMSCs.
METHODS: The articles related to cytological markers and tracer technology of BMSCs from CNKI and Foreign Medical Journal Service database between 1982 and 2011 were retrieved by computer with the key words of “bone marrow mesenchymal stem cells, markers, tracer technique” in title and summary or “ bone marrow, labeling, Tracer” as search terms in English. Finally 29 articles were included.
RESULTS AND CONCLUSION: There are many tracer techniques of BMSCs, the mainly techniques are isotope tracer method, antigen labeling, fluorescent protein, fluorescence dye labeling, magnetic resonance imaging contrast enhancer notation, Lac-Z gene marker method and Y-chromosome labeling. Each method has its advantages and disadvantages. Selected markers should have high specificity, high sensitivity, lower effect on the cell or organism, labeling and detection easily, appropriate marker time and so on. Tracer technique of seed cells still needs further study.
 

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