中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 977-980.doi: 10.3969/j.issn.1673-8225.2012.06.006

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞来源成骨细胞培养体系中加入α-玉米赤霉醇干预后的骨保护素表达***

刘未华1，段  斐1，李少春1，孙晓芳1，郝华超1，何  静1，薛  娟1，邓  鑫1，罗雷雷1，郭文龙2   

  1. 1河北大学基础医学院，河北省保定市  071000；2涞水县医院急诊科，河北省涞水县074100
  • 收稿日期:2011-12-01 修回日期:2012-01-04 出版日期:2012-02-05 发布日期:2012-02-05
  • 通讯作者: 段斐，女，博士，教授，博士生导师，河北大学基础医学院，河北省保定市 071000 feiduan9999@sina.com
  • 作者简介:刘未华，男，1979年生，河北省保定市人，汉族，2004年河北医科大学毕业，助教，主要从事人体解剖学方面的研究。 doctorliu09@163.com
  • 基金资助:

    河北省人口与计划生育委员会项目(2010-A03)；河北大学医学部预研资助项目(2007JY04)；河北大学医学部学科专项资金建设项目(2012A2002）。

Effects of α-zeranol on expression of osteoprotegerin in bone marrow mesenchymal stem cells-derived osteoblasts  

Liu Wei-hua1, Duan Fei1, Li Shao-chun1, Sun Xiao-fang1, Hao Hua-chao1, He Jing1, Xue Juan1, Deng Xin1, Luo Lei-lei1, Guo Wen-long2   

  1. 1School of Basic Medical Science, Hebei University, Baoding  071000, Hebei Province, China; 2Department of Emergency, Laishui Hospital, Laishui  074100, Hebei Province, China
  • Received:2011-12-01 Revised:2012-01-04 Online:2012-02-05 Published:2012-02-05
  • Contact: Duan Fei, Doctor, Professor, Doctoral supervisor, School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China feiduan9999@sina.com
  • About author:Liu Wei-hua, Teaching assistant, School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China doctorliu09@163.com
  • Supported by:

    Population and Family Planning Commission Project of Hebei Province, No. 2010-A03*; Pre-research Fund Project of Hebei University School of Basic Medical Science, No.2007JY04*; Special Fund for Subject Construction Project of Hebei University School of Basic Medical Science No.2012A2002*

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摘要：

背景：研究显示α-玉米赤霉醇对绝经后骨质疏松症具有明显的防治作用。
目的：观察α-玉米赤霉醇对体外大鼠成骨细胞骨保护素分泌及其mRNA表达的影响。
方法：将体外培养的成骨细胞中分别加入雌激素和α-玉米赤霉醇(10-5~10-10 mol/L)进行干预。
结果与结论：ELISA法和RT-PCR检测发现，不同浓度的α-玉米赤霉醇(10-5~10-10 mol/L)作用于成骨细胞后，骨保护素的分泌量及细胞骨保护素mRNA表达较对照组均增加(P < 0.05)。结果证实，在骨髓基质细胞来源的成骨细胞中加入α-玉米赤霉醇可促进成骨细胞骨保护素的分泌及其mRNA的表达。

关键词: 成骨细胞, 破骨细胞, 骨保护素, &alpha, -玉米赤霉醇, 绝经后骨质疏松症, 干细胞因子

Abstract:

BACKGROUND: Studies showed that α-zeranol (α-ZAL) has significant prevention and treatment effect on postmenopausal osteoporosis.
OBJECTIVE: To observe the effects of α-ZAL on the secretion of osteoprotegerin (OPG) and expression of OPG mRNA in osteoblasts of the rats.
METHODS: Estrogen and α-ZAL (10-5-10-10 mol/L) were added into in vitro cultured osteoblasts respectively for intervention.
RESULTS AND CONCLUSION: The results of ELISA and RT-PCR showed that after application of different concentrations of α-ZAL (10-5-10-10 mol/L), the secretion of OPG and expression of OPG mRNA in the estrogen group were higher than those in the control group (P < 0.05). The results confirmed that in the bone marrow mesenchymal stem cells-derived osteoblast, adding α-ZAL can promote osteoblast OPG secretion and mRNA expression.
 

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