中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 875-878.doi: 10.3969/j.issn.1673-8225.2012.05.028

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

pifithrin-α抑制大鼠肝缺血再灌注早期PUMA蛋白的表达*★

彭松林，邢  飞，王  凯，赵  阳，戴朝六   

  1. 中国医科大学附属盛京医院肝胆脾外科，辽宁省沈阳市  110004
  • 收稿日期:2011-08-29 修回日期:2011-12-06 出版日期:2012-01-29 发布日期:2012-01-29
  • 通讯作者: 戴朝六，博士，教授，主任医师，中国医科大学附属盛京医院肝胆外科，辽宁省沈阳市 110004 daicl@sj-hospital.org
  • 作者简介:彭松林★，男，1973年生，湖北省阳新县人，汉族，2005年中国医科大学毕业，硕士，副教授，副主任医师，主要从事肝脏外科临床和研究工作。psl514@126.com
  • 基金资助:

    中国医科大学附属盛京医院院内基金(M835)。

pifithrin-alpha reduces the expression of PUMA protein in the early stage of liver ischemia-reperfusion in rats

Peng Song-lin, Xing Fei, Wang Kai, Zhao Yang, Dai Chao-liu   

  1. Department of Hepatobiliary Surgery, the Affiliated Shengjing Hospital of China Medical University, Shenyang  110004, Liaoning Province, China
  • Received:2011-08-29 Revised:2011-12-06 Online:2012-01-29 Published:2012-01-29
  • Contact: Dai Chao-liu, Doctor, Professor, Chief physician, Department of Hepatobiliary Surgery, the Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China daicl@sj-hospital.org
  • About author:Peng Song-lin★, Master, Associate professor, Associate chief physician, Department of Hepatobiliary Surgery, the Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China psl514@126.com
  • Supported by:

    Fund of the Affiliated Shengjing Hospital of China Medical University, No. M835*

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摘要：

背景：pifithrin-α是一种可逆性p53抑制剂，应用pifithrin-α抑制p53通路对肝脏缺血再灌注损伤的影响尚不清楚。
目的：探讨核转录因子p53抑制剂对大鼠肝缺血再灌注后PUMA蛋白表达的影响。
方法：96只雄性Wistar大鼠随机分为对照组，缺血再灌注组，缺血再灌注+二甲亚砜溶媒对照组(二甲亚砜组)，缺血再灌注+p53抑制剂pifithrin组(PFT组)。建立70%肝缺血模型，PFT组于60 min的肝血流阻断结束时立即给予pifithrin-α，二甲亚砜组给予等量二甲亚砜溶液，对照组和缺血再灌注组给予等量生理盐水。
结果与结论：大鼠肝缺血再灌注后1，3，6 h肝组织PUMA蛋白表达明显，PFT组可以明显抑制PUMA蛋白的表达，但缺血再灌注24 h PFT组PUMA蛋白的表达高于其他3组。结果可见pifithrin-α对肝脏缺血再灌注损伤有一定保护作用，其通过抑制p53从而诱导PUMA蛋白表达下调主要是在缺血再灌注早期。

关键词: 缺血再灌注损伤, 肝脏, p53, PUMA, 大鼠

Abstract:

BACKGROUND: pifithrin-α is a reversible inhibitor of p53, the effect of pifithrin-α inhibited p53 pathway on hepatic ischemia-reperfusion injury is unclear.
OBJECTIVE: To explore the effect of nuclear transcription factor p53 inhibitor on the expression of PUMA protein after liver ischemia-reperfusion in rats.
METHODS: Ninety-six male Wistar rats were divided into four groups randomly: control group, ischemia-reperfusion group, ischemia-reperfusion+solvent dimethyl sulfoxide (DMSO) group (DMSO group) and ischemia-reperfusion+pifithrin-α group (pifithrin-α group). The 70% liver ischemia model was established. The rats in PFT group were injected with pifithrin-α after 60 minutes, the DMSO group was injected with dimethyl sulfoxide solution and the control group and ischemia-reperfusion group were injected with normal saline in the same dose.
RESULTS AND CONCLUSION: At 1, 3 and 6 hours after ischemia-reperfusion in rat liver, the expression of PUMA protein in liver tissue was obviously, the expression of PUMA protein in pifithrin-α group was inhibited significantly. However, at 24 hours after ischemia-reperfusion, the expression of PUMA protein in pifithrin-α group was higher than that in the other three groups. pifithrin-α can protect liver from ischemia-reperfusion injury through suppressing p53 in the order to decrease the expression of PUMA protein, but pifithrin-α can reduce the expression of PUMA protein only in the early stage after rschemia-reperfusion.

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