中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (3): 471-474.doi: 10.3969/j.issn.1673-8225.2012.03.020

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聚乳酸-羟基乙酸共聚物/Ⅰ型胶原/壳聚糖人工硬脊膜在兔脊髓损伤模型中的应用*☆

白万山，王新伟，袁  文，王占超，梁  磊，王会学   

  1. 解放军第二军医大学附属长征医院脊柱外科，上海市  200003
  • 收稿日期:2011-07-14 修回日期:2011-09-15 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 王新伟，博士，副主任医师，副教授，解放军第二军医大学附属长征医院脊柱外科，上海市 200003
  • 作者简介:白万山☆，男，1980年生，四川省中江县人，汉族，2011年解放军第二军医大学毕业，博士，主治医师，主要从事脊柱专业方面的研究。baiwanshan_01@163.com
  • 基金资助:

    上海市科学技术委员会科研计划项目课题(08411963800)，课题名称：人工硬脊膜非缝合技术修复硬脊膜损伤的相关研究。

Effects of poly(lactic-co-glycolic acid)/type-Ⅰcollagen/chitosan composite membrane in rabbit models of spinal cord injury 

Bai Wan-shan, Wang Xin-wei, Yuan Wen, Wang Zhan-chao, Liang Lei, Wang Hui-xue   

  1. Department of Spinal Surgery, Changzhen Hospital, Second Military Medical University, Shanghai  200003, China
  • Received:2011-07-14 Revised:2011-09-15 Online:2012-01-15 Published:2012-01-15
  • Contact: Wang Xin-wei, Doctor, Associate chief physician, Associate professor, Department of Spinal Surgery, Changzhen Hospital, Second Military Medical University, Shanghai 200003, China
  • About author:Bai Wan-shan☆, Doctor, Attending physician, Department of Spinal Surgery, Changzhen Hospital, Second Military Medical University, Shanghai 200003, China baiwanshan_01@ 163.com
  • Supported by:

     

    the Science and Technology Research Plan of Shanghai Science and Technology Committee, No. 08411963800*

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摘要：

背景：自体组织、异体组织、动物来源的硬脊膜替代材料都难达到降低脊髓损伤后致残率与致死率的修复结果。
目的：观察聚乳酸-羟基乙酸共聚物/Ⅰ型胶原/壳聚糖人工硬脊膜修复大白兔脊髓损伤的疗效。
方法：70新西兰大白兔随机分为4组：假手术组(n=10)：单纯切除椎板，不损伤脊髓；模型组(n=20)：脊髓损伤硬脊膜缺损后未进行修复；壳聚糖组(n=20)：脊髓损伤硬脊膜缺损处植入壳聚糖人工硬脊膜；复合膜组(n=20)：脊髓损伤硬脊膜缺损处植入聚乳酸-羟基乙酸共聚物/Ⅰ型胶原/壳聚糖人工复合膜。
结果与结论：脊髓损伤24 h后，壳聚糖组和复合膜组运动功能评分均明显高于模型组(P < 0.01)，复合膜组运动功能评分高于壳聚糖组(P < 0.05)。脊髓损伤之后潜伏期均有明显延长，模型组、壳聚糖组、复合膜组潜伏期明显高于假手术组，在6 h各组潜伏期有明显增加，在24 h左右到达高峰，而后开始逐渐下降，脊髓损伤2 d后模型组、壳聚糖组、复合膜组潜伏期差异无显著性意义。各组细胞凋亡率均大于假手术组(P < 0.05)，损伤后6，24 h壳聚糖组和复合膜组细胞凋亡率均明显低于模型组(P < 0.05)。结果提示在大白兔脊髓损伤模型中应用聚乳酸-羟基乙酸共聚物/Ⅰ型胶原/壳聚糖人工硬脊膜有利于脊髓损伤恢复。
 

关键词: 聚乳酸-聚乙醇酸共聚物, 壳聚糖, Ⅰ型胶原, 人工硬脊膜, 脊髓损伤

Abstract:

BACKGROUND: Autologous tissue, allograft tissue, and dural substitute materials originated from animals are all difficult to reduce the morbidity and mortality of spinal cord injury.
OBJECTIVE: To study the effects of poly(lactic-co-glycolic acid) (PLGA)/type-Ⅰcollagen/chitosan composite membrane in rabbit models with spinal cord injury.
METHODS: Seventy rabbits were randomly divided randomly into sham-operation group (n=10, simple laminectomy without spinal cord injury), model group (n=20, spinal cord injury without treatment), chitosan group (n=20, spinal cord injury treated with artificial dura mater of chitosan), and composite group (n=20, spinal cord injury treated with PLGA/type-Ⅰcollagen/chitosan composite membrane).
RESULTS AND CONCLUSION: Twenty-four hours after spinal cord injury, the motor behavior scores was higher in the chitosan group and composite group than model group (P < 0.01), and the scores in the composite group were also higher than those in the chitosan group (P < 0.05). The latency of somatosensory evoked potential was longer in the model, chitosan, and composite groups than the sham-operation group after spinal cord injury. The latencies were increased at 6 hours after spinal cord injury and reached the peak at 24 hours, and then began to decrease. Two days after spinal cord injury, the differences in the latencies were insignificant among the model, chitosan and composite groups. The apoptotic rate of the sham-operation group was lower than that of other groups (P < 0.05). At 6 and 24 hours after spinal cord injury, the apoptotic rates of the chitosan and composite groups were lower than that of the model group (P < 0.05). Early surgical intervention with PLGA/type-Ⅰcollagen/chitosan composite membrane can acquire best neurological functional recovery in rabbit models of spinal cord injury.
 

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