中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 299-302.doi: 10.3969/j.issn.1673-8225.2012.02.025

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

大鼠RBL-2H3细胞瞬时感受器电位M7通道参与细胞存活及凋亡**☆

吴毅梅1，江山平2，黄林洁2   

  1. 1深圳大学医学院临床医学系，广东省深圳市  518060；2中山大学附属第二医院呼吸内科，广东省广州市  510120
  • 收稿日期:2011-10-11 修回日期:2011-11-09 出版日期:2012-01-08 发布日期:2012-01-08
  • 通讯作者: 江山平，主任医师，教授，博士生导师，中山大学附属第二医院呼吸内科，广东省广州市 510120 shanpingjiang @126.com
  • 作者简介:吴毅梅☆，女，1979年生，广东省梅县人，汉族，2010年中山大学毕业，博士，讲师，主要从事支气管哮喘的临床与基础研究。 gzmeggiewoo@hotmail.com
  • 基金资助:

    国家自然科学基金项目(81070027)；广东省自然基金项目(10151008901000078)。

Role of transient receptor potential melastatin 7 in rat RBL-2H3 cells survival and apoptosis  

NG Ngai-Mui1, Jiang Shan-ping2, Huang Lin-jie2   

  1. 1Department of Clinical Medicine, School of Medicine, Shenzhen University, Shenzhen  518060, Guangdong Province, China; 2Department of Respiratory Medicine, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou  510120, Guangdong Province, China
  • Received:2011-10-11 Revised:2011-11-09 Online:2012-01-08 Published:2012-01-08
  • Contact: Jiang Shan-ping, Chief physician, Professor, Doctoral supervisor, Department of Respiratory Medicine, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, Guangdong Province, Chinashanpingjiang @126.com
  • About author:NG Ngai-Mui☆，Doctor, Lecturer, Department of Clinical Medicine, School of Medicine, Shenzhen University, Shenzhen 518060, Guangdong Province, China gzmeggiewoo@hotmail.com
  • Supported by:

     the National Natural Science Foundation of China, No. 81070027*; the Natural Science Foundation of Guangdong Province, No. 10151008901000078*

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摘要：

背景：瞬时感受器电位M7是肥大细胞上重要的钙离子通道，但其在肥大细胞存活及凋亡过程中的作用仍未明确。
目的：观察瞬时感受器电位M7通道对大鼠RBL-2H3细胞存活及凋亡的影响。
方法：取对数生长期RBL-2H3细胞，①分别以50，100，200 μmol/L瞬时感受器电位通道阻断剂2-APB进行干预，并以0.1%DMSO干预的细胞及正常培养的细胞作对照。②以瞬时感受器电位M7-siRNA反转录病毒载体转染RBL-2H3细胞，并设立空载体组和正常对照组。
结果与结论：经过100，200 μmol/L的2-APB作用72 h后，RBL-2H3细胞数减少，吸光度值降低(P < 0.05)，细胞凋亡增多，早期凋亡率和总凋亡率增加(P < 0.05)；RBL-2H3细胞转染si-瞬时感受器电位M7 72 h 后，吸光度值降低(P < 0.05)，细胞凋亡增多，早期凋亡率和总凋亡率增加(P < 0.05)。说明瞬时感受器电位M7通道参与了RBL-2H3细胞存活和凋亡过程。
 

关键词: 瞬时感受器电位M7通道, RBL-2H3细胞, RNA干扰, 反转录病毒, 凋亡

Abstract:

BACKGROUND: Transient receptor potential melastatin 7 is an important calcium channel in mast cells. However, the role of transient receptor potential melastatin 7 in mast cell survival and apoptosis is unclear.
OBJECTIVE: To investigate the role of transient receptor potential melastatin 7 in rat RBL-2H3 cells survival and apoptosis.
METHODS: RBL-2H3 cells in logarithmic growth phase were intervened with 50, 100 and 200 μmol/L transient receptor potential channel blocker 2-APB. Cells intervened with 0.1% DMSO and normally cultured cells were used as control. RBL-2H3 cells were transfected with retrovirus vector of transient receptor potential melastatin 7-siRNA. Empty vector group and control group were constructed.
RESULTS AND CONCLUSION: At 72 hour after treated with 100 and 200 μmol/L 2-APB, RBL-2H3 cell number was decreased. The optical density was decreased (P < 0.05). Cell apoptosis increased. Early stage apoptotic rate and total apoptotic rate increased (P < 0.05). At 72 hour after RBL-2H3 cells were transfected with transient receptor potential melastatin 7-siRNA, the optical density was decreased (P < 0.05). Cell apoptosis increased. Early stage apoptotic rate and total apoptotic rate increased (P < 0.05). These findings indicate that transient receptor potential melastatin 7 channel involve in the process of survival and apoptosis in RBL-2H3 cells.

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