中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 235-238.doi: 10.3969/j.issn.1673-8225.2012.02.010

• 骨组织构建 • 上一篇    下一篇

丝裂霉素C影响增生性瘢痕成纤维细胞的凋亡

吴晓明，孙  奎，张宏霞，孙喜平，耿琪瑛，李树松
  

  1. 承德医学院附属医院烧伤整形外科，河北省承德市 067000
  • 收稿日期:2011-10-10 修回日期:2011-12-09 出版日期:2012-01-08 发布日期:2012-01-08
  • 作者简介:吴晓明★，男，1973年生，汉族，辽宁省沈阳市人，硕士，主治医师，主要从事烧伤整形方面的研究。 并列第一作者：孙奎，硕士，主治医师，主要从事烧伤整形方面的研究。 sszxwk@sohu.com

Effects of mitomycin C on apoptosis of hypertrophic scar fibroblasts

Wu Xiao-ming, Sun Kui, Zhang Hong-xia, Sun Xi-ping, Geng Qi-ying, Li Shu-song   

  1. Department of Burnand Plastic Surgery,Affiliated Hospital ofChengde MedicalUniversity, Chengde067000, HebeiProvince, China
  • Received:2011-10-10 Revised:2011-12-09 Online:2012-01-08 Published:2012-01-08
  • About author:Wu Xiao-ming★,Master, Attendingphysician,Department of Burnand Plastic Surgery,Affiliated Hospital ofChengde MedicalUniversity, Chengde067000, HebeiProvince, China sszxwk@sohu.com Sun Kui, Master,Attending physician,Department of Burnand Plastic Surgery,Affiliated Hospital ofChengde MedicalUniversity, Chengde067000, HebeiProvince, China Wu Xiao-ming andSun Kui contributedequally to this study.

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431

被引次数

23

摘要：

背景：丝裂霉素C已逐渐开始应用于治疗增生性瘢痕领域中，但针对丝裂霉素C通过细胞凋亡作用于增生性瘢痕分子机制报道很少。
目的：探讨丝裂霉素C对增生性瘢痕成纤维细胞凋亡的影响。
方法：应用2.5，12.5，50，100和200 mg/L 丝裂霉素C作用于体外培养的增生性瘢痕成纤维细胞，采用流式细胞仪检测成纤维细胞的周期分布和凋亡情况；通过Western blot法检测细胞中Bax和Bcl-2蛋白表达水平。
结果与讨论：丝裂霉素C可使增生性瘢痕成纤维细胞的生长阻滞于G0/G1期，并且能够诱导增生性瘢痕成纤维细胞发生凋亡，有着明显的浓度依赖性。经2.5~200 mg/L丝裂霉素C作用24 h后，增生性瘢痕成纤维细胞中Bax蛋白表达增高，Bcl-2蛋白表达降低(P < 0.05)。说明丝裂霉素C可能通过增加增生性瘢痕成纤维细胞Bax表达，降低Bcl-2表达，促进成纤维细胞凋亡的增加。

关键词: 丝裂霉素C, 成纤维细胞, 增生性瘢痕, Bcl-2；Bax, 细胞凋亡

Abstract:

BACKGROUND: Mitomycin C has been gradually used in hyperplastic scar therapy. But the molecular mechanism underlyingthe effects of mitomycin C on hyperplastic scar via cell apoptosis is reported few.
OBJECTIVE: To explore the effects of mitomycin C on fibroblasts apoptosis in hypertrophic scar.
METHODS: Hypertrophic scar fibroblasts were cultured in vitro with five different concentrations of mitomycin C (2.5, 12.5, 50,100, 200 mg/L). Cell cycle distribution and apoptosis of fibroblasts were detected by Annexin V-PI, and the protein expressionlevels of Bax and Bcl-2 in fibroblasts were detected with Western blotting.
RESULTS AND CONCLUSION: Mitomycin C blocked the growth of hyperplastic scar fibroblasts in G0/G1 period, and inducedapoptosis of hyperplastic scar fibroblasts in an obvious concentration-dependent manner. Bax protein expression levelsincreased and Bcl-2 protein expression levels decreased in hyperplastic scar fibroblasts after treated with 2.5-200 mg/Lmitomycin C for 24 hours (P < 0.05). These findings indicate that mitomycin C has the possibility to promote fibroblasts apoptosisthrough increasing Bax expression and decreasing Bcl-2 expression in hyperplastic scar fibroblasts