中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (1): 171-174.doi: 10.3969/j.issn.1673-8225.2012.01.037

• 干细胞临床实践 clinical practice of stem cells • 上一篇    下一篇

脐血间充质干细胞移植脊髓损伤恢复期患者的免疫功能变化*★

赵  宁1，杨万章1，张  敏1，盛佑祥1，唐  映2   

  1. 1广东医学院附属深圳南山医院康复医学科，广东省深圳市 518052； 2深圳市龙岗中心医院康复医学科，广东省深圳市  518000
  • 收稿日期:2011-07-28 修回日期:2011-09-15 出版日期:2012-01-01 发布日期:2012-01-01
  • 通讯作者: 杨万章，博士，教授，主任医师，硕士生导师,广东医学院附属深圳南山医院康复医学科，广东省深圳市 518052
  • 作者简介:赵宁★，女，1976年生，湖南省株洲市人，瑶族，2007年湖南中医药大学毕业，硕士，主治医师，主要从事临床神经系统及骨关节系统疾病康复的研究。 zhloo7692@yahoo.com.cn
  • 基金资助:

    深圳市科技局资助(200405219)，课题名称：间充质干细胞治疗神经系统疾病的临床研究。

Effect of umbilical cord blood mesenchymal stem cells transplantation on immune function of spinal cord injury patients

Zhao Ning1, Yang Wan-zhang1, Zhang Min1, Sheng You-xiang1, Tang Ying2    

  1. 1Department of Rehabilitation Medicine, Shenzhen Nanshan Affiliated Hospital of Guangdong Medical University, Shenzhen  518052, Guangdong Province, China; 2Department of Rehabilitation Medicine, Longgang Central Hospital of Shenzhen, Shenzhen  518000, Guangdong Province, China
  • Received:2011-07-28 Revised:2011-09-15 Online:2012-01-01 Published:2012-01-01
  • Contact: Yang Wan-zhang, Doctor, Professor, Chief physician, Master’s supervisor, Department of rehabilitation medicine, Shenzhen Nanshan Affiliated Hospital of Guangdong Medical University, Shenzhen 518052, Guangdong Province, China
  • About author:Zhao Ning★, Master, Attending physician, Department of Rehabilitation Medicine, Shenzhen Nanshan Affiliated Hospital of Guangdong Medical University, Shenzhen 518052, Guangdong Province, China zhloo7692@yahoo.com.cn
  • Supported by:

    Foundation of Science and Technology Bureau of Shenzhen, No. 200405219*  

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摘要：

背景：间充质干细胞移植因其具有一定的免疫原性，对脊髓损伤患者免疫功能的影响尚无深入系统的报道。
目的：观察脐血源间充质干细胞移植对脊髓损伤患者免疫功能的影响。
方法：61例脊髓损伤患者采用静脉滴注结合腰椎脊髓蛛网膜下腔注射脐血间充质干细胞悬液治疗。应用流式细胞术及免疫比浊法分别测定患者移植前后T淋巴细胞亚群、免疫球蛋白及补体的含量。
结果与结论：与治疗前相比，患者CD3+、IgA及IgG有下降趋势，差异有显著性意义；CD4+、CD8+、CD4+/CD8+、IgM、C3及C4均有升高或下降趋势，但差异无显著性意义。提示脐血源间充质干细胞移植对细胞及体液免疫不会激活急性免疫应答，移植是安全的，存在负向免疫调节作用的可能，但尚需进一步完善相关指标，扩大样本，明确相关机制。
关键词：脐血源间充质干细胞；细胞移植；脊髓损伤；免疫功能；T淋巴细胞亚群；免疫球蛋白；补体
doi:10.3969/j.issn.1673-8225.2012.01.037

关键词: 脐血源间充质干细胞, 细胞移植, 脊髓损伤, 免疫功能, T淋巴细胞亚群, 免疫球蛋白, 补体

Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) transplantation has certain immunogenicity. However, there was no in-depth and system reports about the effect of MSCs transplantation on spinal cord injury patient’s immune function.
OBJECTIVE: To observe the effects of umbilical cord blood MSCs (UCB-MSCs) transplantation on immune function of spinal cord injury (SCI) patients.
METHODS: Sixty-one spinal cord injury (SCI) patients were treated by intervenous drop infusion and lumbar subarachnoid space puncture to UCB-MSCs. Flow-cytometry and immunoturbidimetry (ITM) were used to detect the content of T lymphocytic subgroup and immune globulin and the alexinic in plasma of all the patients prior and post-transplantation.
RESULTS AND CONCLUSION: Compare with pre-transplantation, the content of CD3+, IgA and IgG was decreased and the differences were significant; The content of CD4+, CD8+, CD4+/CD8+, IgM, C3 and C4 was also changed and the differences were not significant. It indicates that UCB-MSCs transplantation could not activate the immunological response of cell and humoral immunity, so the transplantation is safety. Negative accommodation is exist, but the relevant indicators need a further improvement, expand samples, and clearly relevant mechanism. 

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