中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (52): 9696-9700.doi: 10.3969/j.issn.1673-8225.2011.52.002

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

维药买朱尼对骨关节炎大鼠模型关节软骨中基质金属蛋白酶1和基质金属蛋白酶抑制剂1的影响

张耀武1，方  锐2，刘振峰2，艾力江•阿斯拉2，孟庆才2   

  1. 1新疆医科大学，新疆维吾尔自治区乌鲁木齐市 830054
    2新疆维吾尔自治区中医医院，新疆维吾尔自治区乌鲁木齐市  830000
  • 收稿日期:2011-10-13 修回日期:2011-11-02 出版日期:2011-12-24 发布日期:2011-12-24
  • 通讯作者: 孟庆才，博士，主任医师，博士生导师，新疆维吾尔自治区中医医院，新疆维吾尔自治区乌鲁木齐市 830000 mengqingcai@medmail.com.cn
  • 作者简介:张耀武★，男，1980年生，新疆维吾尔自治区精河县人，汉族，新疆医科大学在读硕士，主要从事中西医治疗骨与关节疾病方面的研究。 yaowuz@yahoo.cn
  • 基金资助:

    新疆维吾尔自治区自治区科技支撑计划项目(200933126)。

Effect of Uygur medicine Maizhuni on the expression of matrix metalloproteinase 1 and tissue inhibitor of matrix metalloproteinase 1 in the articular cartilage of rat osteoarthritis models

Zhang Yao-wu1, Fang Rui2, Liu Zhen-feng2, Ailijiang•Asila2, Meng Qing-cai2   

  1. 1Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
    2Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
  • Received:2011-10-13 Revised:2011-11-02 Online:2011-12-24 Published:2011-12-24
  • Contact: Meng Qing-cai, Doctor, Chief physician, Doctoral supervisor, Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830000, Xinjiang Uygur Autonomous Region, China mengqingcai@medmail.com.cn
  • About author:Zhang Yao-wu★, Studying for master’s degree, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China yaowuz@yahoo.cn
  • Supported by:

    the Key Technology Research and Development Program of Xinjiang Uygur Autonomous Region, No. 200933126*

PDF

395

被引次数

11

摘要：

背景：骨关节炎患者关节软骨的损害与基质金属蛋白酶1和基质金属蛋白酶抑制剂失平衡有关。
目的：观察基质金属蛋白酶1、基质金属蛋白酶抑制剂1在关节软骨中的表达及维药买朱尼对其影响。
方法：将20只SD大鼠采用改良Hulth造模法建立大鼠膝骨关节炎模型，按随机数字表法随机等分为买朱尼组和模型组，建模第2周开始分别灌胃维药买朱尼和生理盐水，连续4周。
结果与结论：相比于模型组，买朱尼组大鼠膝关节软骨退变程度减轻，软骨大体评分及Mankin评分降低(P < 0.05)，且膝关节软骨细胞中基质金属蛋白酶1的表达降低，基质金属蛋白酶抑制剂1的表达增加(P < 0.05)。说明维药买朱尼可以通过下调基质金属蛋白酶1的表达水平、上调抑制剂的表达水平，对软骨产生保护作用。

关键词: 基质金属蛋白酶, 骨关节炎, 关节软骨, 基质金属蛋白酶抑制剂, 买朱尼

Abstract:

BACKGROUND: Articular cartilage damage in osteoarthritis patients is related to the imbalance between matrix metalloproteinase 1 and tissue inhibitors of matrix metalloproteinase.
OBJECTIVE: To investigate the expression of matrix metalloproteinase 1 and tissue inhibitor of matrix metalloproteinase 1 in articular cartilage and the intervention effects of Uygur medicine Maizhuni.
METHODS: Knee osteoarthritis model was constructed using modified Hulth method. A total of 20 healthy SD rats were randomly divided into Maizhuni group and model group. Rats were treated with Uygur medicine Maizhuni or saline by gavage in the 2nd week after model construction for 4 weeks.
RESULTS AND CONCLUSION: Compared with model group, the degree of rat knee joint cartilage degeneration in Maizhuni group was alleviated. The cartilage general score and Mankin score in Maizhuni group was lower than that in the model group (P < 0.05). The expression of matrix metalloproteinase 1 in knee joint chondrocytes of Maizhuni group decreased than that of the control group (P < 0.05); the expression of tissue inhibitor of matrix metalloproteinase 1 in Maizhuni group increased than that in the model group (P < 0.05). These findings indicate that Uyghur medicine Maizhuni may protect articular cartilage by down-regulating the expression of matrix metalloproteinase 1 and up-regulating the expression of tissue inhibitor of matrix metalloproteinase 1.

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