中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9603-9607.doi: 10.3969/j.issn.1673-8225.2011.51.024

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

188Re-碘化油-羧甲基壳聚糖-纳米微粒在荷S180肉瘤小鼠体内分布与代谢

王洪震1，贾正平1，郝彦明1，钱荣勋1，董启榕2，徐又佳2   

  1. 1昆山市第一人民医院，江苏省昆山市 215300
    2苏州大学附属二院，江苏省苏州市215000
  • 收稿日期:2011-08-30 修回日期:2011-10-24 出版日期:2011-12-17 发布日期:2011-12-17
  • 作者简介:王洪震☆，男，1964年生，江苏省连云港市人，汉族，2006年苏州大学毕业，博士，主任医师。 a3878072@ 163.com

In vivo distribution and metabolism of 188Re-iodized oil-carboxymethyl chitosan-nanoparticles in the S180 tumor-bearing mice

Wang Hong-zhen1, Jia Zheng-ping1, Hao Yan-ming1, Qian Rong-xun1, Dong Qi-rong2, Xu You-jia2   

  1. 1First People’s Hospital of Kunshan, Kunshan  215300, Jiangsu Province, China
    2Second Affiliated Hospital of Soochow University, Suzhou  215000, Jiangsu rovince, China
  • Received:2011-08-30 Revised:2011-10-24 Online:2011-12-17 Published:2011-12-17
  • About author:Wang Hong-zhen☆, Doctor, Chief physician, First People’s Hospital of Kunshan, Kunshan 215300, Jiangsu Province, China a3878072@163.com

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摘要：

背景：188Re标记的放射性药物在体内发生 Re核索脱落也不会对人体造成严重的辐射损伤。188Re-碘化油有可能成为一种具有临床应用价值的内照射治疗肿瘤的药物。
目的：研究羧甲基壳聚糖载药纳米微球的制备及在荷S180肉瘤小鼠体内分布及代谢。
方法：将188Re-碘化油通过羧甲基壳聚糖纳米微球包裹，将其注射于荷S180肉瘤小鼠体内，通过 SPECT法观察188Re-碘化油-羧甲基壳聚糖-纳米微粒在荷瘤鼠中的显像。
结果与结论：采用羧甲基壳聚糖-纳米微粒对188Re-碘化油标记率达(94.9±0.2)%；肝、肾是188Re-碘化油-羧甲基壳聚糖-纳米微粒的主要分布器官；骨、肌肉、小肠等脏器摄取较少，且随着时间的延长而下降；脑内未测得放射性，各比值随着时间的延长而有增加的趋势，分别在注射显像剂后6~10 h达到峰值。说明羧甲基壳聚糖-纳米微粒对188Re的包裹效果良好；188Re-碘化油-羧甲基壳聚糖-纳米微粒在正常骨、肌肉、小肠基本无摄取，但48 h浓聚于肉瘤组织内，肿瘤与脾、胃、肠、股骨、肌肉、脂肪组织放射性比值高。

关键词: 羧甲基壳聚糖, 纳米微球, 肉瘤小鼠, 体内分布, 代谢

Abstract:

BACKGROUND: 188Re labeled radiopharmaceuticals in body occur Re nuclear cable off which will not cause serious radiation damage of the body. 188Re-iodized oil may become a kind of clinical application of drugs within the radiation treatment of cancer.
OBJECTIVE: To study preparation of drug-loaded nano-carboxymethyl chitosan microspheres and in vivo distribution and metabolism in the S180 tumor-bearing mice.
METHODS: 188Re-lipiodol was wrapped by carboxymethyl chitosan nanoparticles, the wrapped 188Re-lipiodol was injected into the mice bearing S180 sarcoma. Imaging of 188Re-iodized oil-carboxymethyl chitosan-nanoparticles in the tumor-bearing mice was observed by single photon emission computed tomography.
RESULTS AND CONCLUSION: Labeling rate of 188Re-lipiodol which labeled by carboxymethyl chitosan-nanoparticles was (94.9±0.2)%. 188Re-iodized oil-carboxymethyl chitosan was distributed in liver and kidney; and the intake decreased with time in bone, muscle, small intestine and other organs. Radiation was not measured in brain, and the ratio increased with time, and imaging agent reached to peak at 6 to10 hours after injecting. It is indicated that wrapped effect of 188Re by carboxymethyl chitosan-nanoparticles package is well. 188Re-iodized oil-carboxymethyl chitosan-Nanoparticles in normal bone, muscle and  small intestine is almost not uptaked, but 188Re-iodized oil-carboxymethyl chitosan-Nanoparticles can uptake in sarcoma tissue within 48 hours and the radioactivity ratio of the tumor and spleen, stomach, intestine, femur, muscle and adipose tissue is high.

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