中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (50): 9415-9418.doi: 10.3969/j.issn.1673-8225.2011.50.026

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

长针透刺膝骨关节炎模型大鼠滑膜组织中基质金属蛋白酶3的变化

李石胜1, 2，吴耀持2，张峻峰2，周景辉3，孙懿君2，黄承飞2   

  1. 1上海中医药大学附属龙华医院，上海市住院医师规范化培训基地，上海市  200233
    2上海市第六人民医院，上海市  200233
  • 收稿日期:2011-06-01 修回日期:2011-07-13 出版日期:2011-12-10 发布日期:2011-12-10
  • 通讯作者: 吴耀持，教授，主任医师，博士生导师，上海交通大学附属第六人民医院针推伤科，上海市 200233 wuyaochi@online.sh.cn
  • 作者简介:李石胜★，男，1982年生，江西省九江市人，汉族，2010年上海中医药大学毕业，硕士，医师，主要从事骨关节病、腰椎间盘突出症的相关研究。 lishishengshitou@126.com
  • 基金资助:

    上海市科学技术委员会科研项目(08DZ1973200),上海市卫生局中医药科研基金课题(2008L010A)。

Effect of long needle penetration needling on matrix metalloproteinase 3 in the synovium of knee osteoarthritis model rats

Li Shi-sheng1, 2, Wu Yao-chi2, Zhang Jun-feng2, Zhou Jing-hui3, Sun Yi-jun2, Huang Cheng-fei2   

  1. 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai Residency Standardized Training Base, Shanghai  200233, China; 2Sixth People’s Hospital of Shanghai, Shanghai  200233, China
  • Received:2011-06-01 Revised:2011-07-13 Online:2011-12-10 Published:2011-12-10
  • Contact: Wu Yao-chi, Professor, Chief physician, Doctoral supervisor, Sixth People’s Hospital of Shanghai, Shanghai 200233, China wuyaochi@online.sh.cn
  • About author:Li Shi-sheng★, Master, Physician, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai Residency Standardized Training Base, Shanghai 200233, China lishishengshitou@126.com
  • Supported by:

    the Scientific Research Program of Shanghai Science and Technology Committee, No. 08DZ1973200*; the Scientific Research Foundation for Traditional Chinese Medicine of Shanghai Health Bureau, No. 2008L010A*

PDF

625

被引次数

46

摘要：

背景：研究表明基质金属蛋白酶3在关节软骨退变和破坏中有重要意义。
目的：观察长针透刺与透明质酸钠治疗大鼠膝骨关节炎后，大鼠化膜组织中基质金属蛋白酶3含量变化。
方法：SD大鼠通过膝关节腔内注射1.6%木瓜蛋白酶溶液并驱赶大鼠活动建立膝骨关节炎模型。造模2周后随机分为3组，长针透刺组用直径0.30 mm、长125 mm规格的毫针透刺大鼠犊鼻穴和膝眼穴；药物治疗组关节腔内注射透明质酸钠；模型组不干预。并设立不造模的正常组。
结果与结论：治疗4周后，模型组大鼠滑膜组织基质金属蛋白酶3含量较正常组显著升高(P < 0.05)，长针透刺组、药物治疗组基质金属蛋白酶3含量较模型组显著降低(P < 0.05)，长针透刺组与药物组相近(P > 0.05)。证实长针透刺疗法能有效纠正膝骨关节炎模型大鼠化膜组织中基质金属蛋白酶3的异常表达，这也可能是长针透刺治疗膝骨关节炎的作用原理之一。

关键词: 基质金属蛋白酶3, 双抗体夹心EILSA, 膝骨关节炎, 长针透刺, 大鼠

Abstract:

BACKGROUND: Studies have shown that matrix metalloproteinases 3 plays an important role in articular cartilage degeneration and destruction.
OBJECTIVE: To observe the changes of metalloproteinases 3 expression level in synovium of knee osteoarthritis rats after treated with penetration needling of long needle and sodium hyaluronate.
METHODS: SD rats were injected with 1.6% papain solution to knee joint cavity and were driven to exercise in order to construct knee osteoarthritis model. Rats were randomly assigned into 3 groups in the 2nd week after model construction: penetration needling group, drug group and model group. Penetration needling group were treated by penetration needling with filiform needle, with a diameter of 0.30 millimeters and a length of 125 milimeters. Drug group were injected with sodium hyaluronate to knee joint cavity. Model group received no treatment. And non-model control group were established.
RESULTS AND CONCLUSION: Content of synovium matrix metalloproteinase 3 in model group was significantly increased than that in the control group (P < 0.05) in the 4th week after treatment. The content of matrix metalloproteinase 3 in penetration needling group and drug group was significantly reduced than that in the model group (P < 0.05). Penetration needling group was similar to drug group (P > 0.05). These findings demonstrate that long needle penetration therapy can effectively correct the abnormal expression of matrix metalloproteinase 3 in synovium of knee osteoarthritis model rats. It may be one of the mechanisms of the treatment of knee osteoarthritis by long needle penetration needling.

中图分类号: