中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8551-8555.doi: 10.3969/j.issn.1673-8225.2011.46.001

• 软骨组织构建 cartilage tissue construction •    下一篇

电针后血清对肿瘤坏死因子α诱导软骨细胞凋亡的影响

吴明霞1，李西海2，吴广文2，李  俐1，陈文列3，刘献祥2   

  1. 1福建中医药大学附属第二人民医院针灸科，福建省福州市  350003
    2 福建中医药大学中西医结合研究院，福建省福州市  350108
    3 福建省中西医结合老年性疾病重点实验室，福建省福州市  350108
  • 收稿日期:2011-03-19 修回日期:2011-06-12 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 刘献祥，博士，教授，福建中医药大学中西医结合研究院，福建省福州市 350108 liuxianxiang@ 163.com
  • 作者简介:吴明霞☆，女，1965年生，福建省福州市人，汉族，2010年福建中医药大学毕业，博士，主任医师，教授，主要从事中西医结合防治骨性关节炎的研究。 wumingxiafz@163.com
  • 基金资助:

    国家自然科学基金(30873277)，课题名称：温针干预兔软骨退变的信号转导机制研究；陈可冀中西医结合发展基金(CKJ2008003)，课题名称：温针对兔软骨退变信号转导机制影响的研究。

Effects of serum on tumor necrosis factor alpha induced chondrocyte apoptosis following electro-acupuncture

Wu Ming-xia1, Li Xi-hai2, Wu Guang-wen2, Li Li1, Chen Wen-lie3, Liu Xian-xiang2   

  1. 1Department of Acupuncture and Moxibustion, the Second Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China
    2Fujian Academy of Integrative Medicine, Fuzhou  350108, Fujian Province, China
    3Fujian Senile Disease of Integrative Medicine Key Laboratories, Fuzhou 350108, Fujian Province, China
  • Received:2011-03-19 Revised:2011-06-12 Online:2011-11-12 Published:2011-11-12
  • Contact: Liu Xian-xiang, Doctor, Professor, Fujian Academy of Integrative Medicine, Fuzhou 350108, Fujian Province, China liuxianxiang@163. com
  • About author:Wu Ming-xia☆, Doctor, Chief physician, Professor, Department of Acupuncture and Moxibustion, the Second Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China wumingxiafz@163. com
  • Supported by:

    the National Natural Science Foundation of China, No. 30873277*; Chen Ke-ji Integrative Medicine Development Foundation, No. CKJ2008003*

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摘要：

背景：电针具有活血化瘀、行气止痛、舒筋通络的作用，能有效缓解骨性关节炎的临床症状。
目的：观察电针后血清对肿瘤坏死因子α诱导软骨细胞凋亡的影响。
方法：采用机械-酶消化法分离3周龄SD大鼠膝关节软骨细胞，用10 μg/L的肿瘤坏死因子α诱导体外培养软骨细胞的凋亡，同时给予正常大鼠血清、骨性关节炎大鼠血清、透明质酸钠治疗的骨性关节炎大鼠血清、及电针内、外膝眼5，15，30 min治疗的骨性关节炎大鼠血清进行干预。
结果与结论：电针内、外膝眼15，30 min治疗的骨性关节炎大鼠血清干预24 h，软骨细胞活力显著增高，凋亡显著减少。说明电针后血清能有效抑制肿瘤坏死因子α诱导的软骨细胞凋亡。

关键词: 骨性关节炎, 软骨细胞, 凋亡, 电针后血清, 大鼠

Abstract:

BACKGROUND: Electro-acupuncture (EA) can promote blood circulation, remove blood stasis, regulate vital energy circulation to relieve pain, relieve rigidity of muscles and activate collaterals, and effectively relieve symptoms of osteoarthritis.
OBJECTIVE: To observe the effect of serum on tumor necrosis factor α (TNF-α) induced chontroctye apoptosis following EA.
METHODS: Knee joint chondrocytes from 3-week-old SD rats were isolated using enzymatic digestion and apoptosis induced with 10 μg/L TNF-α in vitro. The normal group rats were routinely raised and did not receive any treatment. The osteoarthritis rats were routinely raised, intraarticularly injected with sodium hyaluronate (0.4 μL/g, once a week, for successive 5 weeks), or given EA at the Neixiyan and Waixiyan ( 5, 15 and 30 min/d), and all rats were interfered with serum, and then the apoptotic rate of the chondrocytes was determined.
RESULTS AND CONCLUSION: After 15 and 30 minutes of EA at the Neixiyan and Waixiyan, osteoarthritis rats were interfered with serum for 24 hours, the activity of chondrocytes was significantly increased and the apoptotic rate of the chondrocytes was significantly decreased. These findings suggest that serum can inhibit TNF-α induced chondrocyte apoptosis following EA.

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