中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8542-8546.doi: 10.3969/j.issn.1673-8225.2011.45.041

• 干细胞临床实践 clinical practice of stem cells • 上一篇    下一篇

脐带间充质干细胞促进骨愈合的体外实验及临床应用1例

李华涛1，边琳芬1，吴胜刚1，李  强1，王黎丽1，戴育成2，刘  洋3   

  1. 1解放军第九八医院烧伤整形科，浙江省湖州市  313000
    2南昌大学医学院第二附属医院分子医学研究中心，江西省南昌市 330000
    3协和华东干细胞基因工程有限公司，浙江省湖州市  313000
  • 收稿日期:2011-08-03 修回日期:2011-09-10 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 戴育成，研究员，南昌大学医学院第二附属医院分子医学研究中心，江西省南昌市330000
  • 作者简介:李华涛☆，男，1970年生，江西省广丰县人，汉族，2000年解放军第二军医大学毕业，博士，副主任医师，主要从事创面修复方面的研究。

In vitro experiment of umbilical cord mesenchymal stem cells to promote bone healing and clinical application in one case

Li Hua-tao1, Bian Lin-fen1, Wu Sheng-gang1, Li Qiang1, Wang Li-li1, Dai Yu-cheng2, Liu Yang3   

  1. 1Burns unit, the 98 Hospital of Chinese PLA, Huzhou  313000, Zhejiang Province, China
    2Molecular Medicine Center of Second Affiliated Hospital of Nanchang University Medical School, Nanchang  330000, Jiangxi Province, China
    3Consonance Eastern China Company of Stem Cell Gene Engineering, Huzhou  313000, Zhejiang Province, China
  • Received:2011-08-03 Revised:2011-09-10 Online:2011-11-05 Published:2011-11-05
  • Contact: Dai Yu-cheng, Researcher, Molecular Medicine Center of Second Affiliated Hospital of Nanchang University Medical School, Nanchang 330000, Jiangxi Province, China
  • About author:Li Hua-tao☆, Doctor, Associate chief physician, Burns unit, the 98 Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China eehuatao@163.com

PDF

382

被引次数

8

摘要：

背景：华通胶来源脐带间充质干细胞比成人间充质干细胞更原始，研究表明其端粒酶活性更高、培养倍增时间更短、分化的细胞谱系更广。
目的：观察脐带间充质干细胞在体外分化为成骨细胞的能力及治疗骨折不愈合的效果。
方法：从脐带Wharton胶获取细胞培养、扩增，取传代细胞行免疫表型测定和成骨细胞诱导分化，并以脐带间充质细胞移植治疗1例骨折感染外露后长期不愈病例。
结果与结论：培养细胞形态类成纤维细胞，可长期稳定培养，传代细胞表达间充质干细胞免疫表型，成骨诱导分化的细胞茜素红染色胞浆中有大量的钙沉积，Von Kossa染色有钙结节形成。患者采用脐带间充质干细胞混悬液外用4次，肉芽组织迅速增生填满窦道并上皮化，12 d创面愈合。说明，脐带间充质干细胞具有高度自我更新能力和分化潜能，能够向成骨细胞分化，将其移植治疗骨不连可以显著改善局部微环境。

关键词: 脐带间充质干细胞, 移植, 成骨细胞分化, 骨折外露, 骨愈合

Abstract:

BACKGROUND: Umbilical cord mesenchymal stem cells (UCMSCs) are more original than adult ones. Studies have shown that UCMSCs have higher telomerase activity, shorter culture doubling time and wider lineage differentiation.
OBJECTIVE: To explore the potency of UCMSCs differentiated into osteoblasts in vitro and their clinical effects on bone nonunion.
METHODS: UCMSCs were isolated from Wharton’s jelly of human umbiIical cord; after serial subculture in vitro, MSCs were tested for their immunophenotype and osteogenic differentiation. Clinical application of UCMSCs transplantation was also performed to a patient with refractory exposed bone fracture evoked by infection.
RESULTS AND CONCLUSION: UCMSCs exhibited fibroblastic morphology and long-term stable culture. These passaged cells presented MSCs immunophenotype and there were a great amount of calcium deposition and nodes in intracytoplasm detected by alizarin red and Von Kossa staining, respectively, after osteogenic differentiation. The patient was treated with UCMSCs suspension for four times, granulation tissue grew rapidly to fulfill the sinus tract and became epithelized. Twelve days after treatment, the fracture was healed. It indicates that UCMSCs have self-renewal and pluripotent differentiation potential and can differentiate into osteoblasts in vitro. For treatment of bone nonunion, UCMSCs has the ability to improve microenvironment.

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