中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8263-8266.doi: 10.3969/j.issn.1673-8225.2011.44.024

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

小鼠卵巢免疫反应产物内GATA-4的表达定位

杨加周1，苗乃周1，赵豫凤1，王艳梅1，景彩霞2，艾庆燕1   

  1. 延安大学医学院，1组织学与胚胎学教研室，2寄生虫教研室，陕西省延安市  716000
  • 收稿日期:2011-04-16 修回日期:2011-05-25 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 艾庆燕，硕士，讲师，延安大学医学院组织胚胎学教研室，陕西省延安市 716000 aqy_2001@163.com
  • 作者简介:杨加周，男，1965年生，汉族，陕西省米脂县人， 1993年延安大学医学院毕业，副教授，主要从事组织学与胚胎学专业及生殖医学研究。 jiazhouy@163.com
  • 基金资助:

    陕西省教育厅专项科研计划项目(2010JK906，2010JK907)；陕西省科技厅自然科学基础项目(2010JM4024)；陕西省延安市科学技术研究发展计划项目(2009KS-12)；延安大学科研计划项目(YD2008-04VEGF)。

Localization of GATA-4 in immune reaction product of mouse ovary

Yang Jia-zhou1, Miao Nai-zhou1, Zhao Yu-feng1, Wang Yan-mei1, Jing Cai-xia2, Ai Qing-yan1   

  1. 1Department of Human Histology and Embryology, 2Department of Parasitology, Yan’an University School of Medicine, Yan’an  716000, Shaanxi Province, China
  • Received:2011-04-16 Revised:2011-05-25 Online:2011-10-29 Published:2011-10-29
  • Contact: Ai Qing-yan, Master, Lecturer, Department of Human Histology and Embryology, Yan’an University School of Medicine, Yan’an 716000, Shaanxi Province, China aqy_2001@163.com
  • About author:Yang Jia-zhou, Associate professor, Department of Human Histology and Embryology, Yan’an University School of Medicine, Yan’an 716000, Shaanxi Province, China jiazhouy@163.com
  • Supported by:

    Special Research Program of the Education Department of Shananxi Province, No. 2010JK906*, 2010JK907*; the Natural Science Foundation of Science and Technology Department of Shaanxi Province, No. 2010JM4024*; Science Technology Research Development Plan of Yan’an City of Shaanxi Province, No. 2009KS-12*; Science Technology Research Plan Program of Yannan University, No. YD2008-04VEGF*

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摘要：

背景：特异性转录因子GATA-4家族对动物生殖细胞分化和生殖过程中多种激素的调节有非常重要的作用。
目的：观察小鼠卵巢内GATA-4免疫反应产物的特征与分布。
方法：选取健康昆明种 6周龄ICR 小鼠6只，取卵巢进行石蜡切片，应用免疫组织化学ABC法，DAB显色观察GATA-4在此时期卵巢中的表达情况。
结果与结论：GATA-4免疫反应细胞在6周龄小鼠的卵巢中均有分布，主要在初级卵泡和次级卵泡的颗粒细胞，在卵泡膜细胞上有少量分布，黄体上未见分布。说明小鼠卵巢内存在GATA-4。

关键词: 卵巢, GATA-4, 免疫组织化学, 小鼠, 卵泡

Abstract:

BACKGROUND: GATA-4 transcription factor plays a critical role in mammalian reproduction.
OBJECTIVE: To explore the characteristics and distribution of GATA-4 in immune reaction product of the ovaries of female mouse.
METHODS: Six 6-week old female mice were selected for this study. Paraffin sections of the ovaries were made and GATA-4 expression was observed by immunohistochemical ABC method and diaminobenzidine visualization.
RESULTS AND CONCLUSION: Positive expression of GATA-4 was primarily found in granulosa cells and thecal cells of the mice, with less expression in theca cells, and no expression in corepus luteum. These findings suggest that GATA-4 exists in mouse ovaries.

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