中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8243-8246.doi: 10.3969/j.issn.1673-8225.2011.44.019

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

无创伤双后肢缺血后处理对移植胰腺缺血再灌注损伤的远隔保护

张召辉1，王为忠2，李  玺1，牛万成1，陈党英1，张云民1   

  1. 1解放军97中心医院普通外科，江苏省徐州市   221004
    2解放军第四军医大学西京医院胃肠外科，陕西省西安市 710032
  • 收稿日期:2011-04-12 修回日期:2011-08-06 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 李玺，硕士，主任医师，硕士研究生导师，解放军97中心医院普通外科，江苏省徐州市 221004 Lixi97@163.com
  • 作者简介:张召辉☆，男，1972年生，安徽省灵璧县人，汉族，2008年解放军第四军医大学毕业，博士，主任，副主任医师，硕士生导师，主要从事胃肠外科及器官移植方面的研究。zzhlyn@163.com

Protective effects of non-wounded leg ischemic postconditioning on ischemic reperfusion injury of donor pancreas graft in rats

Zhang Zhao-hui1, Wang Wei-zhong2, Li Xi1, Niu Wan-cheng1, Chen Dang-ying1, Zhang Yun-min1   

  1. 1Department of General Surgery, the 97th Hospital of Chinese PLA, Xuzhou  221004, Jiangsu Province, China
    2Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an  710032, Shanaxi Province, China
  • Received:2011-04-12 Revised:2011-08-06 Online:2011-10-29 Published:2011-10-29
  • Contact: Li Xi, Master, Chief physician, Master’s supervisor, Department of General Surgery, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China Lixi97@163.com
  • About author:Zhang Zhao-hui☆, Doctor, Associate chief physician, Master’s supervisor, Department of General Surgery, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China zzhlyn@163.com

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摘要：

背景：缺血预处理及缺血后处理是近年来提出减轻缺血再灌注损伤有效方法。
目的：探讨无创伤双后肢缺血后处理对移植胰腺缺血再灌注损伤的影响及机制。
方法：18只糖尿病SD大鼠数字表法随机分为3组，对照组仅行开腹术；缺血再灌注组仅行胰腺移植；缺血后处理组，移植前行非创伤性双后肢缺血后处理。
结果与结论：缺血再灌注组血糖和胰腺组织中丙二醛水平均高于缺血后处理组(P < 0.01)、而超氧化物歧化酶活性低于缺血后处理组(P < 0.01)；与缺血后处理组比较，缺血再灌注组胰腺组织凋亡指数明显增高(P < 0.01)。结果提示，无创伤双后肢缺血后处理对大鼠移植胰的缺血再灌注损伤具有保护作用，机制可能与可通过减少超氧化物歧化酶失活，从而清除氧自由基以及减少胰腺细胞凋亡等有关。

关键词: 胰腺移植, 缺血后处理, 再灌注损伤, 远距离保护, 大鼠

Abstract:

BACKGROUND: Ischemic preconditioning and ischemic postconditioning are effective methods to alleviate ischemia/reperfusion injury.
OBJECTIVE: To investigate the effects of non-wounded leg ischemic postconditioning on ischemic reperfusion injury of donor pancreas graft in rats and to analyze the possible mechanism.
METHODS: The control group consisted of six normal SD rats suffering from sham operation and 12 streptomycin-induced diabetic SD rats were randomly and evenly assigned to two groups: In the ischemia/reperfusion group, rats received pancreas transplantation normally, without additional intervention. In the ischemic postconditioning group, rats underwent non-wounded leg ischemic postconditioning before transplantation. The blood glucose, malondialdehyde (MDA) and superoxide dismutase (SOD) in graft tissue were monitored, and apoptotic cells were stained by TUNEL technique at 2 hours after long-time reperfusion. RESULTS AND CONCLUSION: The blood glucose and MDA in the ischemia/reperfusion group were significantly higher compared with the ischemic postconditioning group after reperfusion (P < 0.01). SOD level in the ischemia/reperfusion group was significantly lower than that in the ischemic postconditioning group (P < 0.01). The apoptotic index in the ischemia/reperfusion group was significantly higher than that in the ischemic postconditioning group (P < 0.01). Results showed that non-wounded leg ischemic postconditioning can protect rat pancreas graft from ischemic reperfusion injury during pancreas transplantation. The possible mechanisms may be related to releasing oxygen radical and reducing cell apoptosis after reperfusion.

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