中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8201-8204.doi: 10.3969/j.issn.1673-8225.2011.44.009

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

自体嗅鞘细胞联合β-七叶皂甙钠修复大鼠脊髓全横断损伤

王  璐，王航辉，刘伯锋，赵  琳   

  1. 延安大学医学院解剖学教研室，陕西省延安市716000
  • 收稿日期:2011-06-19 修回日期:2011-07-21 出版日期:2011-10-29 发布日期:2011-10-29
  • 作者简介:王璐★，男，1972年生，陕西省咸阳市人，汉族，延安大学医学院在读硕士，副教授，主要从事应用解剖学研究。 wl6490@163.com 并列第一作者：王航辉★，男，1977年生，陕西省咸阳市人，汉族，2007年南昌大学毕业，硕士，副教授，主要从事应用解剖学研究。
  • 基金资助:

    陕西省卫生厅科学研究基金项目(2010H25)；陕西省自然科学基金资助项目(08JK502)；延安市科技计划资助项目(2009KS-22)。

Transplantation of autologous olfactory ensheating cells combined with beta-aescinate sodium for repair of spinal cord transection in rats

Wang Lu, Wang Hang-hui, Liu Bo-feng, Zhao Lin   

  1. Department of Anatomy, Medical College of Yan’an University, Yan’an 716000, Shaaxi Province, China
  • Received:2011-06-19 Revised:2011-07-21 Online:2011-10-29 Published:2011-10-29
  • About author:Wang Lu★, Studying for master’s degree, Associate professor, Department of Anatomy, Medical College of Yan’an University, Yan’an 716000, Shaaxi Province, China Wang Hang-hui★, Master, Associate professor, Department of Anatomy, Medical College of Yan’an University, Yan’an 716000, Shaaxi Province, China Wang Lu and Wang Hang-hui contributed equally to this study
  • Supported by:

    Science Research Foundation of Health Department of Shaanxi Province, No. 2010H25*; the Natural Science Foundation of Shaaxi Province, No. 08JK502*; Science and Technology Plan of Yan’an City, No. 2009KS-22*

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摘要：

背景：神经损伤时移植嗅鞘细胞能促进轴突再生，减少星形胶质细胞增生。
目的：探讨自体嗅鞘细胞移植联合中药β-七叶皂甙钠对脊髓继发性损伤的保护作用。
方法：制作SD大鼠脊髓损伤模型，随机分为对照组(注射生理盐水)、自体嗅鞘细胞移植组、自体嗅鞘细胞移植联合β-七叶皂甙钠治疗组。
结果与结论：脊髓损伤后脊髓血管源性水肿、基质金属蛋白酶9表达上调，大鼠运动功能缺失，经自体嗅鞘细胞移植及自体嗅鞘细胞联合β-七叶皂甙钠治疗后脊髓水肿明显减轻、基质金属蛋白酶9表达下调，大鼠运动功能恢复，以自体嗅鞘细胞移植联合β-七叶皂甙钠治疗效果更明显。说明自体嗅鞘细胞移植联合β-七叶皂甙钠可更有效修复损伤脊髓，发挥保护作用。

关键词: 脊髓损伤, 自体移植, 嗅鞘细胞, 基质金属蛋白酶9, 中药

Abstract:

BACKGROUND: Transplantation of olfactory ensheating cells (OECs) can promote axon regeneration and reduce the proliferation of astrocytes in spinal cord injury.
OBJECTIVE: To explore the protective effects and mechanism of transplantation of autologous OECs combined with β-aescinate sodium in spinal cord injury rats. METHODS: Healthy Sprague-Dawley rats were divided into three groups: control, autologous OECs transplantation (OECs), transplantation of autologous OECs combined with β-aescinate sodium (OECs + β-aescinate sodium).
RESULTS AND CONCLUSION: Vasogenic spinal cord edema and matrix metalloproteinase 9 expression were increased, and rat motor function was lost. After transplantation of autologous OECs combined with β-aescinate sodium, spinal cord edema was obviously alleviated, MMP-9 expression was downregulated, rat motor function was recovered. These symptoms were obvious in the OECs + β-aescinate sodium group. Results showed that transplantation of autologous OECs combined with β-aescinate sodium can effectively repair spinal cord injury and show protective effects.

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