中国组织工程研究

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (2): 172-177.doi: 10.3969/j.issn.2095-4344.2970

• 骨组织构建 bone tissue construction • 上一篇    下一篇

三七总皂苷干预去势骨质疏松性骨折模型大鼠的作用机制

胡  广，关智宇，张开伟   

  1. 贵州中医药大学第一附属医院骨科，贵州省贵阳市   550001
  • 收稿日期:2019-12-30 修回日期:2020-01-07 接受日期:2020-03-06 出版日期:2021-01-18 发布日期:2020-11-21
  • 通讯作者: 关智宇，博士，硕士生导师，主任医师，贵州中医药大学第一附属医院骨科，贵州省贵阳市 550001
  • 作者简介:胡广，男，1982年生，湖北省黄梅县人，汉族，2012年贵州医科大学毕业，硕士，副主任医师，硕士生导师，主要从事骨折以及骨肿瘤的研究。
  • 基金资助:
    国家自然科学基金项目(81960813)

Mechanism underlying the interventional effect of Panax Notoginsenosides on ovariectomized osteoporotic fracture rats

Hu Guang, Guan Zhiyu, Zhang Kaiwei    

  1. Department of Orthopedics, the First Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, Guiyang 550001, Guizhou Province, China
  • Received:2019-12-30 Revised:2020-01-07 Accepted:2020-03-06 Online:2021-01-18 Published:2020-11-21
  • Contact: Guan Zhiyu, MD, Master’s supervisor, Chief physician, Department of Orthopedics, the First Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, Guiyang 550001, Guizhou Province, China
  • About author:Hu Guang, Master, Associate chief physician, Master’s supervisor, Department of Orthopedics, the First Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, Guiyang 550001, Guizhou Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81960813

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摘要：

文题释义：
三七总皂苷：中药三七主治活血祛瘀，通脉活络；具有抑制血小板聚集和增加脑血流量的作用；可用于脑血管后遗症、视网膜中央静脉阻塞、眼前房出血等。三七总皂苷是三七的有效成分之一，活血作用强大，对骨的代谢有显著的影响。
骨质疏松性骨折：骨质疏松症是多种原因引起的以骨量降低、骨组织微观结构退化为主要病理特征的疾病，可导致骨脆性增高，骨折危险性增加。流行病学统计显示，绝经后骨质疏松症是最常见的原发性骨质疏松症，绝经后女性的患病率接近50%，与同龄非骨质疏松者比，骨质疏松症导致骨折风险增加至少20%。如何改善骨质疏松，降低骨质疏松症导致的骨折发生率及加速骨质疏松骨折的愈合是骨科的研究热点。

摘要
背景：骨质疏松性骨折是最常见的由骨质疏松引起的严重并发症，其修复较为困难，严重威胁患者身体健康、生活质量。
目的：使用三七总皂苷对去势骨质疏松性骨折大鼠进行干预，研究相关作用机制。
方法：选取50只健康雌性大鼠，10只为正常组，其余40只建立去势骨质疏松性骨折模型，分为模型组、三七总皂苷低、中、高剂量组，正常组和模型组大鼠灌胃生理盐水，三七总皂苷低、中、高剂量组大鼠分别灌胃10，20，40 mg/kg三七总皂苷溶液。实验于2019-09-23经贵州中医药大学第一附属医院动物实验伦理委员会批准，批准号为(2019)伦审第(58)号。
结果与结论：三七总皂苷高剂量组大鼠骨密度、骨细胞指数均高于低、中剂量组(P < 0.05)。高剂量组大鼠骨体积分数、骨痂骨小梁数量、骨小梁厚度均高于模型组、三七总皂苷低、中剂量组，骨小梁分离度低于模型组、三七总皂苷低、中剂量组(P < 0.05)。三七总皂苷高剂量组大鼠谷胱甘肽过氧化物酶、骨形成发生蛋白2、血管内皮生长因子水平低于正常组，高于模型组、三七总皂苷低、中剂量组，过氧化脂、丙二醛、骨钙素、Ⅰ型前胶原羧基端前肽、骨特异性碱性磷酸酶水平高于正常组，低于模型组以及三七总皂苷低、中剂量组(P < 0.05)。三七总皂苷低、中、高剂量组大鼠PI3K、Akt、mTOR表达均低于正常组、高于模型组，且三七总皂苷高剂量组大鼠PI3K、Akt、mTOR表达均高于三七总皂苷低、中剂量组(P < 0.05)。提示使用三七总皂苷干预后，去势骨质疏松性骨折大鼠骨密度、骨细胞指数上升，促进骨痂处骨小梁生长，减轻大鼠氧化应激损伤，调控PI3K/Akt/mTOR信号通路，促进骨痂新生血管形成及骨折愈合。
关键词：实验；动物；鼠；因子；蛋白；通路；骨质疏松；中药
https://orcid.org/0000-0002-4948-4582 (关智宇) 

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 实验, 动物, 鼠, 因子, 蛋白, 通路, 骨质疏松, 中药

Abstract: BACKGROUND: Osteoporotic fractures are the most common serious complications caused by osteoporosis, and their repair is more difficult, which seriously threatens the health and quality of life of patients. 
OBJECTIVE: To investigate the interventional effect of Panax Notoginsenosides in ovariectomized osteoporotic fracture rats and the relevant mechanism. 
METHODS: Fifty healthy female rats were selected. Ten of them were normal group, and the other 40 rats were used to make ovariectomized osteoporotic fracture models. Model rats were divided into model group, low, medium and high dose group. Normal group and model group rats were intragastrically administered normal saline, rats in the low, medium and high dose group were given intragastric administration of 10, 20 and 40 mg/kg Panax Notoginsenosides solution. The study protocol was approved by the Animal Ethic Committee of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. (2019)58 on September 23, 2019.
RESULTS AND CONCLUSION: The bone mineral density and bone cell index of the high dose group were higher than those of low and middle dose groups             (P < 0.05). The bone volume fraction, the number of callus trabeculae and the thickness of trabeculae in the high dose group were all higher than those in the model group, the low dose group and the middle dose group, and the resolution of trabeculae was lower than that in these three groups (P < 0.05). The levels of glutathione peroxidase, bone morphogenetic protein 2, and vascular endothelial growth factor in the high dose group were lower than those in the normal group, but higher than those in the model group, low and medium dose groups. The levels of lipid peroxide, malondialdehyde, osteocalcin, type I procollagen carboxy terminal propeptide, bone-specific alkaline phosphatase in the high dose group were higher than those in the normal group, but lower than those in the model group, low and medium dose group (P < 0.05). The expression of PI3K, Akt and mTOR in the low, middle and high dose groups was lower than that in the normal group and higher than that in the model group; and the expression of PI3K, Akt and mTOR in the high dose group was higher than that in the low and middle dose groups (P < 0.05). Therefore, treatment with Panax Notoginsenosides can increase the bone miner density and bone cell index, promote the growth of bone trabecula at the callus, alleviate oxidative stress injury, regulate the PI3K / Akt / mTOR signal pathway, and accelerate the formation of new blood vessels in the callus and fracture healing in ovariectomized osteoporotic fracture rats. 

Key words: experiment, animal, rat, factor, protein, pathway, osteoporosis, Chinese herb

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