中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (42): 7822-7826.doi: 10.3969/j.issn.1673-8225.2011.42.008

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

多孔碳酸钙陶瓷与干细胞源性成骨细胞的相容性

魏任雄，张  翼，谭金海，蔡  林，金  伟   

  1. 武汉大学中南医院骨科，湖北省武汉市  430071
  • 收稿日期:2011-07-04 修回日期:2011-08-12 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 谭金海，博士，教授，主任医师，硕士生导师，武汉大学中南医院骨科，武汉大学中南医院骨科，湖北省武汉市 430071 guke3224@ yahoo.com.cn
  • 作者简介:魏任雄☆，男，1980年生，湖北省黄梅县人，汉族，2009年武汉大学医学院毕业，博士，主治医师，主要从事骨肿瘤与骨组织工程研究。 Feiyi030@ 163.com
  • 基金资助:

    湖北省自然科学基金重点项目(2009CDA058)

Cytocompatibility of stem cells-derived osteoblasts with porous calcium carbonate ceramics

Wei Ren-xiong, Zhang Yi, Tan Jin-hai, Cai Lin, Jin Wei   

  1. Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan  430071, Hubei Province, China
  • Received:2011-07-04 Revised:2011-08-12 Online:2011-10-15 Published:2011-10-15
  • Contact: Tan Jin-hai, Doctor, Chief physician, Professor, Master’s supervisor, Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China guke3224@ yahoo.com.cn
  • About author:Wei Ren-xiong☆, Doctor, Attending physician, Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China Feiyi030@163.com
  • Supported by:

    the Key Grant of Natural Science Foundation of Hubei Province, No. 2009CDA058*

PDF

245

被引次数

3

摘要：

背景：国内外的研究证实普通碳酸钙陶瓷作为骨替代材料时具有细胞支架作用。
目的：观察多孔碳酸钙陶瓷与成骨细胞的相容性，及作为骨组织工程支架的可能性。
方法：SD大鼠骨髓基质干细胞经矿化诱导培养、扩增并检测证实其已具成骨细胞表型后，分别与多孔碳酸钙陶瓷支架、普通羟基磷灰石陶瓷支架体外复合培养。
结果与结论：骨髓基质干细胞经体外诱导形成成骨细胞，钙结节、Ⅰ型胶原和碱性磷酸酶免疫染色结果阳性。多孔碳酸钙陶瓷支架材料与羟基磷灰石陶瓷材料皆有细胞附着生长，但多孔碳酸钙陶瓷支架材料细胞的黏附能力、增殖活力及成骨活性均强于羟基磷灰石陶瓷材料。提示多孔碳酸钙陶瓷支架材料与SD大鼠骨髓基质干细胞源性成骨细胞有良好相容性。

关键词: 多孔碳酸钙, 陶瓷支架, 骨髓基质干细胞, 相容性, 大鼠

Abstract:

BACKGROUND: Foreign studies have confirmed that calcium carbonate ceramics as bone substitute materials has a cytoskeletal role.
OBJECTIVE: To study the cytocompatibility of osteoblasts with porous calcium carbonate ceramics as bone tissue engineering scaffold.
METHODS: Bone marrow stromal cells (BMSCs) obtained from SD rat bone marrow were in vitro induced and proliferated. After their osteoblast phenotypes were verified, osteoblasts which cultured from BMSCs were seeded onto prepared porous calcium carbonate ceramics (PCCC group) and common hydroxyapatite ceramics (HA group).
RESULTS AND CONCLUSION: Immunological staining results for calcium nodules, Ⅰ collagen and alkaline phosphatase were positive. Adherent osteoblasts were found in the two groups. However, the adherent ability, proliferative ability and osteogenic activity in the PCCC group were stronger than those in the HA group. It indicates that the porous calcium carbonate ceramics has good cytocompatibility with BMSCs-derived osteoblasts.

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