中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7643-7646.doi: 10.3969/j.issn.1673-8225.2011.41.011

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

长期力竭运动动情周期抑制模型大鼠卵巢组织形态和超微结构的变化

付  燕1，周妙荣1，熊若虹2   

  1. 1西南民族大学，四川省成都市 610041
    2成都体育学院，四川省成都市  610041
  • 收稿日期:2011-07-01 修回日期:2011-08-02 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 付燕★，女，1972年生，重庆市人，汉族，2006年成都体育学院毕业，硕士，主治医师，讲师，主要从事运动保健研究。 fuyan1010@163.com
  • 作者简介:付燕★，女，1972年生，重庆市人，汉族，2006年成都体育学院毕业，硕士，主治医师，讲师，主要从事运动保健研究。 fuyan1010@163.com
  • 基金资助:

    中央高校基本科研业务费专项资金学术梯队建设工程项目(项目编号: 11SZYTD15)

Changes in histomorphology and ultrastructure of ovaries in exercise-induced estrus disorder rats

Fu Yan1, Zhou Miao-rong1, Xiong Ruo-hong2   

  1. 1Southwest University for Nationalities, Chengdu  610041, Sichuan Province, China
    2 Chengdu Sport University, Chengdu  610041, Sichuan Province, China
  • Received:2011-07-01 Revised:2011-08-02 Online:2011-10-08 Published:2011-10-08
  • Contact: Fu Yan, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China fuyan1010@163.com
  • About author:Fu Yan★, Master, Attending physician, Lecturer, Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China fuyan1010@163.com
  • Supported by:

    Academic Team Construction Project Program of Basic Scientific Research Business Special Foundation of Central Higher Education Institute, No. 11SZYTD15*

PDF

276

被引次数

7

摘要：

背景：研究运动性月经失调女性性腺和卵巢的功能，以及形态学的改变对于探讨运动性月经失调的发生机制具有重要意义。
目的：通过建立长期力竭运动致动情周期抑制大鼠模型，观察卵巢的组织形态和细胞超微结构的变化。
方法：正常3月龄SD雌性大鼠随机分成对照组和模型组，其中模型组大鼠每日进行一次力竭游泳运动直至其动情周期紊乱，取卵巢组织，苏木精-伊红染色，光镜下观察卵巢组织形态变化，电镜下观察卵巢细胞超微结构的变化。
结果与结论：与对照组相比，模型组大鼠卵巢典型生长卵泡和新鲜黄体明显减少，原始卵泡、闭锁卵泡则相对增加，其颗粒细胞、黄体细胞内细胞器明显减少而含有大量的脂滴。提示长期力竭运动可抑制大鼠卵巢卵泡的发育、成熟、排卵与黄体形成，并使卵巢细胞的超微结构发生抑制性改变。

关键词: 长期力竭运动, 动情周期抑制, 卵巢, 组织形态, 超微结构, 大鼠

Abstract:

BACKGROUND: It has important significance to research the changes in ovarian function and structures in women with athletic menstrual cycle irregularities. But at present, reports about ovarian structures in women with athletic menstrual cycle irregularities are less.
OBJECTIVE: To investigate the changes in histomorphology and ultrastructure of ovaries in rats with estrus irregularities resulting from long-term exhaustive swimming and to gain more insight into mechanisms underlying athletic menstrual cycle irregularities.
METHODS: Twenty-four female Sprague-Dawley rats, aged 3 months old, having normal estrus cycles were divided randomly into the control group and the model group with 12 rats in each group. The rats in the model group underwent exhaustive swimming every day until those estrous cycles were disordered. Hematoxylin-eosin staining and transmission electron microscopy were used to investigate the changes in histomorphology and ultrastructure of rat ovaries.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that compared with the control group, typical growing follicles, mature follicles and fresh corpus luteum were relatively reduced, primordial and atretic follicles were relatively increased in the model group. Transmission electron microscope results showed that compared with the control group, less organelle and more lipid droplets both in granulocytes and lutein cells could be observed in the model group. The findings demonstrated that long-term exhaustive sports can inhibit follicular development, maturation, ovulation and corpus luteum formation and result in inhibitory changes of ultrastructure of ovarian cells. This may be one of mechanisms of athletic menstrual cycle irregularities.

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