中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7611-7614.doi: 10.3969/j.issn.1673-8225.2011.41.003

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

胰岛素样生长因子1与转化生长因子β2促进组织工程软骨构建的体外实验

陈宁杰1, 2，张  伟1，梁晓琴2，杨彪炳2   

  1. 1 潍坊医学院整形外科学研究所，山东省潍坊市  261042
    2 潍坊医学院整形外科医院整形美容科，山东省潍坊市  261042
  • 收稿日期:2011-03-01 修回日期:2011-05-01 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 杨彪炳，主任医师，教授，硕士生导师，潍坊医学院整形外科医院整形美容科，山东省潍坊市 261042 ybiaobing@163.com
  • 作者简介:陈宁杰★，男，1984年生，山东省潍坊市人，汉族，2008年潍坊医学院毕业，硕士，主要从事软骨组织工程的研究。 cnfly101942@ 163.com
  • 基金资助:

    山东省高等学校科技计划项目(J09LF20)，课题名称：藻酸钙凝珠载体软骨细胞立体培养及调控因素的研究。

Insulin-like growth factor 1 and transformation growth factor beta 2 promote engineered cartilage formation in vitro

Chen Ning-jie1, 2, Zhang Wei1, Liang Xiao-qin2, Yang Biao-bing2   

  1. 1Plastic Surgery Research Institution, Weifang Medical College, Weifang 261042, Shandong Province, China
    2Department of Aesthetic Plastic Surgery, Plastic Surgery Hospital of Weifang Medical College, Weifang  261042, Shandong Province, China
  • Received:2011-03-01 Revised:2011-05-01 Online:2011-10-08 Published:2011-10-08
  • Contact: Yang Biao-bing, Chief physician, Professor, Master’s supervisor, Department of Aesthetic Plastic Surgery, Plastic Surgery Hospital of Weifang Medical College, Weifang 261042, Shandong Province, China ybiaobing@163.com
  • About author:Chen Ning-jie★, Master, Pastic Surgery Research Institution, Weifang Medical College, Weifang 261042, Shandong Province, China; Department of Aesthetic Plastic Surgery, Plastic Surgery Hospital of Weifang Medical College, Weifang 261042, Shandong Province, China cnfly101942@163. com
  • Supported by:

    Science and Technology Development Program of Higher Education of Shandong Province, No.J09LF20*

PDF

294

摘要：

背景：软骨组织的再生能力差，软骨组织工程能利用较少的细胞、支架材料和细胞因子对缺损进行修复。
目的：观察胰岛素样生长因子1与转化生长因子β2联合应用对组织工程软骨形成的影响。
方法：用酶消化法获取人软骨细胞，将培养的细胞以4×109 L-1的细胞浓度接种在藻酸钙凝珠支架上，分别加入200 μg/L胰岛素样生长因子1和(或)1 μg/L转化生长因子β2进行立体培养。于培养的第3，5，7，9，11，13天，进行细胞计数，观察软骨细胞的增殖情况。培养2周后，进行大体形态观察和阿尔新蓝-过碘酸雪夫氏染色 (AB-PAS)及抗Ⅱ型胶原免疫组织化学染色。
结果与结论：细胞计数及免疫组织化学染色显示，胰岛素样生长因子1和转化生长因子β2均能促进软骨细胞增殖和软骨相关基质黏多糖及Ⅱ型胶原的分泌，其中胰岛素样生长因子1的作用主要体现在促细胞增殖方面，而转化生长因子β2的作用主要体现在促进软骨相关基质形成方面，二者联合应用具有促进组织工程软骨形成的协同作用。

关键词: 胰岛素样生长因子1, 转化生长因子&beta, 2, 软骨, 藻酸钙, 软骨组织工程

Abstract:

BACKGROUND: Cartilage tissue has poor regenerative capacity. Cartilage tissue engineering can repair damaged cartilage tissue using fewer cells, scaffold materials and cytokines. 
OBJECTIVE: To investigate the effects of insulin-like growth factor (IGF-1) combined with transformation growth factor beta 2 (TGF-β2) on engineered cartilage formation.
METHODS: Human chondrocytes were isolated by enzyme digestion method and then were inoculated onto calcium alginate bead scaffold for culture in three-dimensional circumstance after addition of 200 μg/L IGF-1 and (or) 1 μg/L TGF-β2. At 3, 5, 7, 9, 11, and 13 days of culture, chondrocyte proliferation was tested by cell counting. After 2 weeks of culture, the fresh tissues were assessed by morphological observation, alcian blue-periodic acid sthiff staining, and anti typeⅡcollagen immunohistochemicel staining.
RESULTS AND CONCLUSION: IGF-1 and TGF-β2 could stimulate chondrocytes to proliferate and excrete chondral matrix. IGF-1 mainly promoted the proliferation of chondrocytes, while TGF-β2 mainly promoted the formation of cartilage-related matrix. IGF-1 combined with TGF-β2 has synergistic effects on promoting engineered cartilage formation.

中图分类号: