中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7105-7108.doi: 10.3969/j.issn.1673-8225.2011.38.017

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

聚丁二炔纳米粒对大肠埃希氏菌的免疫检测

郭讯枝1,2，何剪太1，张阳德1，周伟华2   

  1. 1中南大学国家卫生部肝胆肠外科研究中心，中南大学生物医学工程研究院，湖南省长沙市 410008
    2宜春学院江西省天然药物活性成分研究重点实验室，江西省宜春市 336000
  • 收稿日期:2011-06-16 修回日期:2011-07-11 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 周伟华，副教授，宜春学院江西省天然药物活性成分研究重点实验室，江西省宜春市 336000 subdeb@163.com
  • 作者简介:郭讯枝☆，女，1972年生，湖南省韶山市人，汉族，中南大学在读博士，讲师，主要从事纳米生物技术研究。
  • 基金资助:

    江西省教育厅科技项目(GJJ09359)和江西省科技支撑计划资助项目(2009BSA12400)。

Immunodetection to Escherichia coli of polydiacetylene nanoparticles

Guo Xun-zhi1,2, He Jian-tai1, Zhang Yang-de1, Zhou Wei-hua2   

  1. 1National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha  410008, Hunan Province, China
    2 Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun  336000, Jiangxi Province, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun  336000, Jiangxi Province, China
  • Received:2011-06-16 Revised:2011-07-11 Online:2011-09-17 Published:2011-09-17
  • Contact: Zhou Wei-hua, Associate professor, Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China subdeb@163.com
  • About author:Guo Xun-zhi☆, Studying for doctorate, Lecturer, National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha 410008, Hunan Province, China；Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China
  • Supported by:

    the Science and Technology Project of Jiangxi Education Bureau, No. GJJ09359*; the Scientific Supporting Program of Jiangxi Province, No. 2009BSA12400*

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322

被引次数

4

摘要：

背景：采用PCR、ELISA、免疫荧光球法，以及基因芯片技术进行肠道病原菌的快速检测，需要数小时才能得到结果，不适用于现场快速检验。
目的：制备聚丁二炔纳米粒，对大肠埃希氏菌进行免疫检测。
方法：采用超声分散法制备聚丁二炔纳米粒，并利用其检测大肠埃希氏菌。
结果与结论：制备的聚丁二炔纳米粒粒径均匀，分布范围窄，结构稳定。取大肠埃希氏菌稀释液滴入聚丁二炔免疫纳米粒稀释液中2 min，溶液颜色由蓝变红，表明聚丁二炔纳米粒的检测速度快；可见吸收光谱图、透射电镜图、激光粒径分布图均显示，加入大肠埃希氏菌后聚丁二炔免疫纳米粒变化很大。在利用聚丁二炔免疫纳米粒比色检测大肠埃希氏菌的过程中，其鼠抗大肠埃希氏菌多克隆抗体起到了功能分子的作用，抗原-抗体结合反应是特异性的。

关键词: 聚丁二炔, 纳米粒, 10, 12二十五碳二炔酸(PCDA), 金黄色葡萄球菌蛋白A, 大肠埃希氏菌

Abstract:

BACKGROUND: PCR, ELISA, immunofluorescence-ball method, and gene chip technology for rapid detection of enteric pathogens take hours to get results and are not suitable for on-site rapid test.
OBJECTIVE: To prepare polydiacetylene (PDA) nanoparticles for immunodetection to Escherichia coli.
METHODS: PDA nanoparticles were prepared with sonication.
RESULTS AND CONCLUSION: The prepared PDA nanoparticles had uniform particle size, narrow distribution, and stable structure. Escherichia coli dilution was dropped into PDA nanoparticle dilution for 2 minutes, and the solution color turned from blue to red, indicating that PDA nanoparticles were rapid in detection speed. The graphs of ultraviolet-visible absorption spectroscopy, transmission electron microscope, and nanosizer displayed big changes with Escherichia coli and antibody PDA nanoparticle. The experimental results signed that the rat polyclonal antibody is a functional molecule of the immunodetection to Escherichia coli of PDA nanoparticles, and the reaction of antigen-antibody is specific.

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