中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7066-7070.doi: 10.3969/j.issn.1673-8225.2011.38.009

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

3-羟基丁酸与3-羟基己酸共聚酯的血管内生物相容性

吴  松1，刘迎龙2，唐  跃2，王  强2，万  峰1，曲向华3，陈国强3   

  1. 1北京大学第三医院心脏外科，北京市  100191
    2中国医学科学院心血管病研究所 北京协和医学院阜外心血管病医院心血管外科，北京市  100037
    3清华大学生物系微生物研究室，北京市  100084
  • 收稿日期:2011-03-01 修回日期:2011-05-24 出版日期:2011-09-17 发布日期:2011-09-17
  • 作者简介:吴松☆，男，1968年生，北京市人，汉族，2007年北京协和医学院毕业，博士，主治医师，主要从事心血管外科临床和心血管组织工程基础研究。

Intravascular biocompatibility of poly (3-hydroxybutyrate- co-3-hydroxyhexanoate)

Wu Song1, Liu Ying-long2, Tang Yue2, Wang Qiang2, Wan Feng1, Qu Xiang-hua3, Chen Guo-qiang3   

  1. 1Department of Cardiovascular Surgery, Peking University Third Hospital, Beijing  100191, China
    2Department of Cardiovascular Surgery, Cardiovascular Institute & Fuwai Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing  100037, China
    3Department of Biological Science and Biotechnology, Tsinghua University, Beijing  100084, China
  • Received:2011-03-01 Revised:2011-05-24 Online:2011-09-17 Published:2011-09-17
  • About author:Wu Song☆, Doctor, Attending physician, Department of Cardiovascular Surgery, Peking University Third Hospital, Beijing 100191, China drwusong@gmail.com

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386

被引次数

2

摘要：

背景：可降解聚合材料3-羟基丁酸与3-羟基己酸共聚酯(3-hydroxybutyrate-co- 3-hydroxyhexanoate, PHBHHx)具有良好的机械性能和生物可降解性。
目的：在体研究PHBHHx的血管内生物相容性。
方法：采用脱细胞羊肺动脉为支架，以PHBHHx涂层，构建复合补片，植入新西兰兔腹主动脉内，以脱细胞未涂层羊肺动脉片作为对照。分别于植入后第1，4，12周取出移植补片进行组织学、免疫荧光染色、扫描电镜和钙含量测定。
结果与结论：复合补片管腔面光滑无血栓，内膜增生适度，再细胞化完全；免疫荧光染色可见新生内膜组织中类内皮细胞呈CD31阳性反应，单层连续排列，间质细胞呈平滑肌肌动蛋白阳性反应；复合补片的钙含量明显低于未涂层羊肺动脉片。说明PHBHHx的血管内生物相容性满意，是心血管组织工程较为理想的腔内涂层材料。

关键词: 脱细胞异种血管, 3-羟基丁酸与3-羟基己酸共聚酯, 血管内生物相容性, 心血管组织工程

Abstract:

BACKGROUND: The degradable poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) has superior mechanical property and biocompatibility.
OBJECTIVE: To elucidate the intravascular biocompatibility of PHBHHx in vivo.
METHODS: We developed hybrid materials based on decellularized xenogenic vascular scaffolds that were coated with PHBHHx and implanted it into the abdominal aorta of New Zealand rabbits. The decellularized xenogenic pulmonary artery patch without PHBHHx coating served as the control. The implanted patches were determined for the histology, immunofluorescence staining, scanning electron microscopy and calcium contents at 1, 4 and 12 weeks after the surgery.
RESULTS AND CONCLUSION: Hybrid patches exhibited smooth lumen surface without thrombus, the intimal hyperplasia was mild and recellularization was complete; immunofluorescence staining showed that the endothelial cells in the neointima were positive for CD31, with continuous single-layer arrangement, interstitial cells were positive for smooth muscle actin; the calcium content in hybrid patches was obviously lower than that in uncoated patches. PHBHHx shows a remarkable intravascular biocompatibility in vivo and is believed as an ideal candidate for lumen coating of cardiovascular tissue engineering.

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