中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7041-7044.doi: 10.3969/j.issn.1673-8225.2011.38.003

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

新型脱细胞骨基质材料的制备及理化评估

杨  强1, 2，彭  江1，夏  群2，马信龙2，徐宝山2，郭全义1，汪爱媛1，赵  斌1，张  莉1，许文静1，卢世璧1   

  1. 1解放军总医院全军骨科研究所，北京市  100853 
    2天津市天津医院脊柱科，天津市   300211
  • 收稿日期:2011-03-06 修回日期:2011-04-20 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 卢世璧，院士，博士生导师，解放军总医院全军骨科研究所，北京市100853 shibilu301@ gmail.com 并列通讯作者：彭江，副教授，解放军总医院全军骨科研究所，北京市100853 pengjdxx@ 126.com
  • 作者简介:杨强☆，男，1980年生，山东省青州市人，汉族，2008年解放军总医院毕业，博士，主治医师，主要从事脊柱外科、骨科组织工程研究。 yangqiang198@126.com
  • 基金资助:

    国家自然科学青年基金(31000432)，课题名称：“基于股骨髁Micro-CT图像的仿生骨软骨双层支架的研制及动物实验研究”；国家自然科学基金重点课题资助(30930092)，课题名称：仿生生物陶瓷支架构建的组织工程骨软骨复合体防止股骨头坏死塌陷的研究；天津市卫生局科技基金(09KZ67)，课题名称：“骨软骨双层支架联合脂肪干细胞构建组织工程骨软骨复合体”。

Fabrication and characterization of a novel acellular bone matrix scaffold for bone tissue engineering

Yang Qiang1, 2, Peng Jiang1, Xia Qun1, Ma Xin-long2, Xu Bao-shan2, Guo Quan-yi1, Wang Ai-yuan1, Zhao Bin1, Zhang Li1, Xu Wen-jing1, Lu Shi-bi1   

  1. 1Institute of Orthopedics, Chinese PLA General Hospital, Beijing  100853, China
    2Department of Spinal Surgery, Tianjin Hospital, Tianjin  300211, China
  • Received:2011-03-06 Revised:2011-04-20 Online:2011-09-17 Published:2011-09-17
  • Contact: Lu Shi-bi, Academician, Doctoral supervisor, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China shibilu301@ gmail.com Correspondence to: Peng Jiang, Associate professor, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China pengjdxx@126.com
  • About author:Yang Qiang☆, Doctor, Attending physician, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China; Department of Spinal Surgery, Tianjin Hospital, Tianjin 300211, China yangqiang1980@ 126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30930092*, 31000432*; Research Found of Tianjin Health Bureau, No. 09KZ67*

PDF

507

被引次数

6

摘要：

背景：传统的结构性天然骨脱细胞的方法存在许多不足之处。
目的：用新的理化方法对结构性骨块进行脱细胞处理制作新型骨支架材料的可行性，并检测其理化性能。
方法：以股骨头负重区结构性骨块为原料，高压水枪冲洗，继而利用Triton-100、脱氧胆酸钠等进行脱细胞等理化处理，制备新型脱细胞骨基质材料，并对支架进行大体、组织学、扫描电镜、Micro-CT观察、生物力学等相关检测。
结果与结论：新型脱细胞骨基质材料保留了骨的细胞外基质成分，脱细胞彻底，扫描电镜及Micro-CT观察显示支架具备三维多孔网状结构系统，具有天然骨的孔径和孔隙率；生物力学测试脱细胞组支架的弹性模量为(552.56±58.92) MPa，强度为(11.34±3.49) MPa，与新鲜骨的弹性模量及强度比较，差异无显著性意义(P > 0.05)，可作为骨组织工程支架的良好载体。

关键词: 骨, 组织工程, 细胞外基质, 支架, 生物力学

Abstract:

BACKGROUND: Natural bone acellular cells have many inadequacies.
OBJECTIVE: To fabricate and characterize a novel acellular bone matrix scaffold for bone tissue engineering, and to detect its physical and chemical properties.
METHODS: Bone column was prepared using canine bone cancellous bone from the femoral head, washed with high-pressure water gun, and decellularized using TritonX-100 and sodium deoxycholatethe. The scaffold was investigated by histological staining, scanning electronic microscope observation, Micro-CT and biomechanical analysis.
RESULTS AND CONCLUSION: The new bone scaffold showed most of the extracellular matrix components after removal of the cell fragments after decellularization. Scanning electron microscopy and Micro-CT revealed a three-dimensional interconnected porous structure, with the pore diameter and porosity of nature bone. The longitudinal elastic modulus of the new scaffold was (552.56±58.92) MPa, and the intensity was (11.34±3.49) MPa. There was no significant difference between the natural bone and the new scaffold (P > 0.05). The new acellular bone matrix scaffold is a good alternative cell-carrier for bone tissue engineering.

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