中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6937-6941.doi: 10.3969/j.issn.1673-8225.2011.37.023

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

人颈椎间盘退变与细胞凋亡及基质金属蛋白酶11的表达

郭团茂1，刘  淼2，张银刚2，张小卫2，武世勋2   

  1. 1咸阳市中心医院骨科，陕西省咸阳市  712000
    2西安交通大学医学院第一附属医院骨科，陕西省西安市  710061
  • 收稿日期:2011-03-02 修回日期:2011-04-23 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 张银刚，博士，副教授，硕士生导师，西安交通大学医学院第一附属医院骨科，陕西省西安市 710061 zyingang@mail. xjtu.edu.cn
  • 作者简介:郭团茂☆，男，1975年生，陕西省西安市人，汉族，2011年西安交通大学毕业，博士，主治医师，主要从事椎间盘退变性疾病的研究。 gtm0619@163. com
  • 基金资助:

    国家自然科学基金(30400163)，课题名称：骨髓间质干细胞椎间盘内异位分化及差异基因的评价；陕西省科技计划项目(2008K14-01)，课题名称：人退变颈椎间盘组织基因表达谱的分析。

Matrix metalloproteinase 11 expression and cell apoptosis in human cervical disc degeneration

Guo Tuan-Mao1, Liu Miao2, Zhang Yin-Gang2, Zhang Xiao-Wei2, Wu Shi-Xun2   

  1. 1Department of Orthopedics, Xianyang Central Hospital, Xianyang  712000, Shaanxi Province, China
    2Department of Orthopedics, First Affiliated Hospital, Medical College of Xi’an Jiao Tong University, Xi’an  710061, Shaanxi Province, China
  • Received:2011-03-02 Revised:2011-04-23 Online:2011-09-10 Published:2011-09-10
  • Contact: Zhang Yin-gang, Doctor, Associate professor, Master’s supervisor, Department of Orthopedics, First Affiliated Hospital, Medical College of Xi’an Jiao Tong University, Xi’an 710061, Shaanxi Province, China zyingang@mail.xjtu. edu.cn
  • About author:Guo Tuan-mao☆, Doctor, Attending physician, Department of Orthopedics, Xianyang Central Hospital, Xianyang 712000, Shaanxi Province, China gtm0619@163.com
  • Supported by:

    the National Natural Science Foundation of China, No.30400163*; Science and Technology Plan Program of Shaanxi Province, No.2008K14-01*

PDF

270

被引次数

6

摘要：

背景：前期研究发现基质金属蛋白酶11基因在人退变颈、腰椎间盘组织中明显上调。
目的：观察人退变颈椎间盘髓核组织中基质金属蛋白酶11的表达与细胞凋亡的关系。
方法：纳入30个经MRI确认的退变颈椎间盘髓核组织和20个因颈椎创伤治疗获得的正常颈椎间盘髓核组织。
结果与结论：苏木精-伊红染色显示退变的颈椎间盘髓核组织中髓核细胞较正常髓核组织明显减少(P < 0.01)，而凋亡细胞较正常髓核组织明显增多(P < 0.01)。免疫组化染色显示退变的颈椎间盘髓核组织中基质金属蛋白酶11的表达明显高于正常髓核组织(P < 0.01)，且基质金属蛋白酶11表达与TUNEL染色检测到的细胞凋亡正相关(r=0.44，P < 0.05)。说明高表达的基质金属蛋白酶11不仅可直接破坏细胞外基质尚可诱导髓核细胞凋亡，在椎间盘退变的过程中发挥重要作用。

关键词: 颈椎间盘, 髓核, 退变, 细胞凋亡, 基质金属蛋白酶11

Abstract:

BACKGROUND: Previous studies have shown that matrix metalloproteinase 11 gene is obviously upregulated in human degenerated cervical and lumbar disc tissues.
OBJECTIVE: To investigate the relationship between matrix metalloproteinase 11 expression in human cervical intervertebral discs and cell apoptosis.
METHODS: Thirty discs from patients with cervical spondylosis diagnosed by MRI, and 20 discs of individuals with cervical spinal trauma but without anamnesis evidence for disc degeneration or previous spine-ralated operations were included.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that nucleus pulposus cells were signifcantly reduced  (P < 0.01), but apoptotic cells were significantly increased (P < 0.01) in the degenerated nucleus pulposus tissue of cervical intervertebral discs than in the normal nucleus pulposis tissue. Immunohistochemical staining showed that matrix metalloproteinase 11 expression was significantly greater (P < 0.01) in the degenerated nucleus pulposus tissue of cervical intervertebral discs than in the normal nucleus pulposis tissue, and matrix metalloproteinase 11 expression was positively related to cell apoptosis detected by TUNEL(r=0.44, P < 0.05). Results showed that high expression of matrix metalloproteinase 11 can directly damage extracellular matrix, induce nucleus pulposis cell apoptosis, and therefore play an important role in the process of disc degeneration.

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