中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6923-6926.doi: 10.3969/j.issn.1673-8225.2011.37.020

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

不同浓度富硒温泉水对增生性瘢痕成纤维细胞增殖的影响

胡清泉1，熊  慧2，邹立津3，张友来3，李郁葱1，曾元临3   

  1. 1 南昌大学医学院，江西省南昌市  333006
    2 江西省护理职业技术学院，江西省南昌市  330006
    3 南昌大学第一附属医院烧伤中心，江西省南昌市  330006
  • 收稿日期:2011-03-08 修回日期:2011-04-28 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 曾元临，教授，主任医师，南昌大学第一附属医院烧伤中心，江西省南昌市330006 zengyuanlin777@yahoo.com.cn
  • 作者简介:胡清泉★，男，1986年生，江西省上饶市人，汉族，南昌大学医学院在读硕士，医师，主要从事烧伤创面的处理与瘢痕防治的研究。 huqingquan@ 126.com
  • 基金资助:

    江西省卫生厅2007 年计划课题项目，编号：20073220，课题名称：富硒温泉水浸渍对烧伤疤痕和表皮干细胞的影响程度研究。

Effects of different doses of selenium-rich water on the proliferation of hypertrophic scar fibroblasts

Hu Qing-quan1, Xiong Hui2, Zou Li-jin3, Zhang You-lai3, Li Yu-cong1, Zeng Yuan-lin3   

  1. 1Medical College of Nanchang University, Nanchang  333006, Jiangxi Province, China
    2Jiangxi Vocational and Technical College of Nursing, Nanchang  330006, Jiangxi Province, China
    3Burn Center, First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Received:2011-03-08 Revised:2011-04-28 Online:2011-09-10 Published:2011-09-10
  • Contact: Zeng Yuan-lin, Professor, Chief physician, Burn Center, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China zengyuanlin777@ yahoo.com.cn
  • About author:Hu Qing-quan★, Studying for master’s degree, Physician, Medical College of Nanchang University, Nanchang 333006, Jiangxi Province, China huqingquan@126. com
  • Supported by:

    2007 Plan Program of Health Department of Jiangxi Province, No.20073200*

PDF

297

被引次数

3

摘要：

背景：临床研究证实，富硒温泉水对增生性瘢痕有明显的抑制作用。
目的：观察富硒温泉水对人增生性瘢痕成纤维细胞增殖的影响。
方法：组织块法体外培养人增生性瘢痕成纤维细胞，取第3~5代细胞，用含10%，20%，30%富硒温泉水及自来水和蒸馏水的培养基培养48 h，测细胞增殖情况。
结果与结论：富硒温泉水培养的人增生性瘢痕成纤维细胞数量减少，突起不同程度的变短或者缺失，细胞质及胞核浓缩。不同浓度的富硒温泉水、自来水、蒸馏水均对成纤维细胞的增殖有抑制作用，以富硒温泉水培养基的抑制作用最明显 (P < 0.05)，且随浓度的升高，抑制率增大。说明富硒温泉水可以剂量依赖性地抑制人增生性瘢痕成纤维细胞的增殖。

关键词: 富硒温泉水, 硒, 瘢痕, 成纤维细胞, 增殖, 组织工程

Abstract:

BACKGROUND: Clinical studies have shown that selenium-rich water can obviously inhibit hypertrophic scar.
OBJECTIVE: To investigate the effects of selenium-rich water on the proliferation of human hypertrophic scar fibroblasts (HSFBs).
METHODS: Human HSFBs were cultured by the tissue explants method. Passages 3-5 human HSFBs were cultured with 10%, 20% and 30% selenium-rich water, running water and distilled water for 48 hours. Cell proliferation was measured by MTT method.
RESULTS AND CONCLUSION: Human HSFBs cultured with selenium-rich water were reduced in number, cell processes were shortened to different extents or lacked, and cytoplasm and nucleus were reduced. Different doses of selenium-rich water, running water, and distilled water exhibited inhibitory effects on the proliferation of human HSBFs, in particular selenium-rich water and in a dose-dependent manner (P < 0.05). Results showed that selenium-rich water inhibits the proliferation of human HSFBs in a dose-dependent manner.

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