中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6718-6721.doi: 10.3969/j.issn.1673-8225.2011.36.016

• 肿瘤干细胞 cancer stem cells • 上一篇    下一篇

胃癌肿瘤干细胞的分离与成脂分化

匡  弢1，王  雷2，宋  文3，孟冬梅4   

  1. 青岛大学医学院附属医院，1特需保健科，3放射科，4青岛市常见病重点实验室—痛风病实验室，山东省青岛市  266003；2青岛市立医院血液科，山东省青岛市  266071
  • 收稿日期:2011-04-29 修回日期:2011-07-29 出版日期:2011-09-03 发布日期:2011-09-03
  • 通讯作者: 孟冬梅，博士，主治医师，青岛大学医学院附属医院痛风病实验室，山东省青岛市 266003 mengdongmeiqd@gmail.com
  • 作者简介:匡弢，男，1963年生，山东省胶州市人，汉族，1986年上海第二军医大学毕业，副主任医师，主要从事老年肿瘤专业的研究方面。 kuangtk@126.com

Isolation and aipogenic differentiation of gastric cancer stem cells

Kuang Tao1, Wang Lei2, Song Wen3, Meng Dong-mei4   

  1. 1Health Care Department, 3Department Radiology, Affiliated Hospital of Qingdao University Medical School, Qingdao  266003, Shandong Province, China; 2Department of Hematology, Qingdao Municipal Hospital, Qingdao  266071, Shandong Province, China; 4Qingdao Key Laboratory of Common Diseases-Gout Laboratory, Qingdao  266003, Shandong Province, China
  • Received:2011-04-29 Revised:2011-07-29 Online:2011-09-03 Published:2011-09-03
  • Contact: Meng Dong-mei, Doctor, Attending physician, Qingdao Key Laboratory of Common Diseases-Gout Laboratory, Qingdao 266003, Shandong Province, China mengdongmeiqd@gmail.com
  • About author:Kuang Tao, Associate chief physician, Health Care Department, Affiliated Hospital of Qingdao University Medical School, Qingdao 266003, Shandong Province, China kuangtk@126.com

PDF

321

被引次数

21

摘要：

背景：肿瘤干细胞是否存在于所有肿瘤中仍有争议。
目的：从人胃癌细胞中分离并鉴定类肿瘤干细胞，对胃癌的发生机制进行探讨。
方法：原代培养胃癌细胞，并通过流式细胞术检测CD44和CD29等细胞因子的阳性表达率，分选阳性细胞进行成脂诱导。
结果与结论：胃癌细胞中CD44、CD29的阳性百分率分别为5.67%和5.53%，CD44和CD29阳性细胞具有成脂能力，证明胃癌肿瘤细胞中存在具有分化能力的类肿瘤干细胞。

关键词: 肿瘤干细胞, 胃癌细胞, 成脂分化, 生物学性状, CD44, CD29

Abstract:

BACKGROUND: Whether cancer stem cells are present in all tumors is still controversial.
OBJECTIVE: To isolate and identify cancer stem cells from human gastric cancer cells, and to explore the mechanism of gastric cancer.
METHODS: Gastric cancer cells were primarily cultured and CD44 and CD29 positive expression rates were detected by flow cytometry to sort positive cells for adipogenic induction.
RESULTS AND CONCLUSION: CD44 and CD29 positive percentages in gastric cancer cells were 5.67% and 5.53%, respectively, indicating that CD44 and CD29 positive cells had an adipogenic capacity. It is proved that there are tumor stem cells with differentiation capacity in gastric cancer cells.

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