中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6714-6717.doi: 10.3969/j.issn.1673-8225.2011.36.015

• 肿瘤干细胞 cancer stem cells • 上一篇    下一篇

组织芯片观察CD44+/CD166+结直肠癌干细胞的形态及分布

罗  雁1，2，3，刘  斌1，2，安  宁4，董  亮1，钱  震1，张永东3   

  1. 1甘肃省干细胞与基因药物重点实验室，解放军兰州军区兰州总医院病理科，甘肃省兰州市   730050
    2兰州大学基础医学院病理教研室，甘肃省兰州市 730030
    3甘肃省医学科学研究院，甘肃省兰州市 730050
    4甘肃省肿瘤医院，甘肃省兰州市  730050
  • 收稿日期:2011-03-02 修回日期:2011-03-31 出版日期:2011-09-03 发布日期:2011-09-03
  • 通讯作者: 刘斌，主任医师，教授，硕士生导师，解放军兰州军区兰州总医院病理科，甘肃省兰州市730050 liumb@189.cn
  • 作者简介:罗雁★，女，1982 年生，浙江省上虞市人，汉族，兰州大学在读硕士，主要从事临床肿瘤病理的研究。 Luoy1982@yeah.net
  • 基金资助:

    甘肃省自然科学基金资助项目(096RJZA096)。

Observation of morphology and distribution of CD44+/CD166+ colorectal cancer stem cells by tissue microarray

Luo Yan1,2,3, Liu Bin1,2, An Ning4, Dong Liang1, Qian Zhen1, Zhang Yong-dong3   

  1. 1Key Laboratory of Stem Cell and Gene Medicine, Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou  730050, Gansu Province, China
    2Department of Pathology, Basic Medical College, Lanzhou University, Lanzhou  730030, Gansu Province, China
    3Gansu Academy of Medical Science, Lanzhou 730050, Gansu Province, China
    4Tumor Hospital of Gansu Province, Lanzhou  730050, Gansu Province, China
  • Received:2011-03-02 Revised:2011-03-31 Online:2011-09-03 Published:2011-09-03
  • Contact: Liu Bin, Chief physician, Professor, Master’s supervisor, Key Laboratory of Stem Cell and Gene Medicine, Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China; Department of Pathology, Basic Medical College, Lanzhou University, Lanzhou 730030, Gansu Province, China liumb@189.cn
  • About author:Luo Yan★, Studying for master’s degree, Key Laboratory of Stem Cell and Gene Medicine, Department of Pathology, Lanzhou General Hospital of Lanzhou Military Command of Chinese PLA, Lanzhou 730050, Gansu Province, China; Department of Pathology, Basic Medical College, Lanzhou University, Lanzhou 730030, Gansu Province, China; Gansu Academy of Medical Science, Lanzhou 730050, Gansu Province, China Luoy1982@yeah.net
  • Supported by:

    the Natural Science Foundation of Gansu Province, No. 096RJZA096*

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摘要：

背景：越来越多的研究将CD44作为结直肠癌干细胞的一个特异性的标志物。
目的：观察结直肠癌干细胞CD44+/CD166+在原发性结直肠癌组织中的数量、位置及分布方式。
方法：取61份结直肠癌、10份正常肠黏膜组织及18份伴不典型增生的结直肠腺瘤标本制成42点的共3块组织芯片。
结果与结论：免疫组织化学双染结果显示，正常肠黏膜中未见CD44+/CD166+细胞，在伴不典型增生腺瘤中可见极少量CD44+/CD166+细胞，结直肠癌中可见到少量CD44+/CD166+双阳性细胞。双阳性细胞主要分布在结直肠癌腺管基底侧，呈灶状或散在分布；细胞呈立方形，胞浆稀少，胞核规则，呈卵圆形或高柱状，随着结直肠癌分化程度的降低，数量增多，且其数量在浸润较深的癌巢中较多。提示结直肠癌干细胞的数量、位置、分布方式及其形态有助于结直肠癌的诊断及判断预后。

关键词: 结直肠癌, 肿瘤干细胞, CD44+/CD166+, 组织芯片, 免疫组织化学双重染色法

Abstract:

BACKGROUND: More and more research employs CD44 as a specific marker of colorectal cancer stem cells.
OBJECTIVE: To investigate the quantity, location and distribution of colorectal cancer stem cells (Co-CSCs) CD44+/CD166+ in primary colorectal carcinoma.
METHODS: A totally of 61 cases of human colorectal carcinoma and 10 cases of normal mucosa, 18 cases of adenoma were collected and made into three tissue microarrays each containing of 42 dots respectively.
RESULTS AND CONCLUSION: The results of double-label immunohistochemical staining demonstrated there was no CD44+/CD166+ cell in normal intestine mucosa, a very small amount of CD44+/CD166+ cells in adenoma, also double-positive cells could be seen in colorectal carcinoma, the number of double-positive cells was rare and the cells were scattered or distributed focally along the basement of gland basal side. The cells with scarcely cytoplasm were square, and its nucleus was oval or high cylindrical, deep stained and homogeneous. The quantity of double-positive cells was negatively correlated with the differentiation of colorectal carcinoma. The more depth was infiltrated, the more quantity of double-positive cells was observed. To observe the number, location, distribution and morphology of Co-CSCs can contribute to the diagnosis and prognostic evaluation of colorectal carcinoma.

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