中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6257-6261.doi: 10.3969/j.issn.1673-8225.2011.33.043

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

聚集蛋白聚糖基因多态性与腰椎间盘突出症的关联性

谭平先1, 2，李  健3，孙筱放4，颜登鲁3，张在恒3，张慧敏4   

  1. 1东莞市常平医院骨科，广东省东莞市523573；2中山大学附属第一医院骨科，广东省 广州市 510080；广州医学院第三附属医院，3骨科，4妇产科研究室，广东省广州市510150
  • 收稿日期:2011-03-06 修回日期:2011-06-09 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 李健，教授，主任医师，硕士生导师。广州医学院第三附属医院骨科，广东省广州市510150 heavyyoung@ yahoo.com.cn
  • 作者简介:谭平先，1972年生，男，汉族，河南省人，中山大学在读博士，副主任医师，主要从事脊柱与关节外科的诊治工作。 tanpingx@ yahoo.cn

Intragenic polymorphisms of the aggrecan associated with lumbar disk herination

Tan Ping-xian1, 2, Li Jian3, Sun Xiao-fang4, Yan Deng-lu3, Zhang Zai-heng3, Zhang Hui-min4   

  1. 1Department of Orthopedics, Changping Hospital of Dongguan City, Dongguan 523573, Guangdong Province, China; 2Department of Orthopedics, First Affiliated Hospital of Sun Yat-sen University, Guangzhou   510080, Guangdong Province, China ; 3Department of Orthopedics, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou   510150, Guangdong Province, China; 4Department of Gynaecology and Obstetrics, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou   510150, Guangdong Province, China
  • Received:2011-03-06 Revised:2011-06-09 Online:2011-08-13 Published:2011-08-13
  • About author:Tan Ping-xian☆, Studying for doctorate, Associate chief physician, Department of Orthopedics, Changping Hospital of Dongguan City, Dongguan 523573, Guangdong Province, China; Department of Orthopedics, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China tanpingx@yahoo.cn

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419

被引次数

4

摘要：

背景：课题组以往研究表明，中国南方地区汉族人群聚集蛋白聚糖基因存在串联重复序列多态性。
目的：观察聚集蛋白聚糖基因多态性与腰椎间盘突出症的关联性。
方法：纳入椎间盘突出症患者和非椎间盘突出症患者各51例。应用聚合酶链反应检测两组聚集蛋白聚糖基因多态性分布情况。采用分层χ2检验分析聚集蛋白聚糖基因多态性在非椎间盘突出症组合腰椎间盘突出症组之间的分布差异。
结果与结论：两组聚集蛋白聚糖基因多态性分布情况检测发现，聚集蛋白聚糖等位基因分布以中等长度串联重复序列片段最多见，短和长串联重复序列片段较少见，两组中出现频率最多的基因型是等位基因27，两组聚集蛋白聚糖等位基因多态性频率分布差异无显著性意义(P > 0.05)。在基因型的分布上两组变化趋势基本相同，非椎间盘突出症患者和椎间盘突出症患者分别得出7和6种长短不同的串联重复序列。结果证实，聚集蛋白聚糖基因多态性与腰椎间盘突出症尚无明确关联。

关键词: 聚集蛋白聚糖, 基因多态性, 腰椎, 椎间盘突出, 骨组织构建

Abstract:

BACKGROUND: Previous studies have shown that aggrecan gene in Han population in southern regions in China shows tandem repetitive sequence polymorphism.
OBJECTIVE: To investigate the association between aggrecan polymorphism and lumbar disk herniation.
METHODS: A total of 102 participants with or without low back pain problem were included in this study. Two groups were used. The study group included 51 lumbar disk herniation participants. The control group included 51 patients without lumbar disk herniation or low back pain or spine diseases. The aggrecan polymorphisms of the two groups were obtained. The Cochran-Mantel-Haenszel test was performed to assess the distributing difference of aggrecan gene polymorphisms between the study group and control group.
RESULTS AND CONCLUSION: Seven alleles were found in the control group, and six alleles were found in the study group. The frequency of allele 27 was the most common form in the study and control groups, and there was no significant difference in the allele polymorphism between these two groups (P > 0.05). The distributing frequency of each allele was basically the same between the control and study groups, i.e., seven and six tandem repetitive sequences of different lengths were found in these two groups, respectively. These findings suggest that no obvious association was found between aggrecan polymorphism and lumbar disc herniation.

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