中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6239-6242.doi: 10.3969/j.issn.1673-8225.2011.33.039

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

基因敲除模型小鼠在疾病研究中的应用

石玉衡   

  1. 南京农业大学食品科技学院，江苏省南京市210095
  • 收稿日期:2011-06-01 修回日期:2011-07-05 出版日期:2011-08-13 发布日期:2011-08-13
  • 作者简介:石玉衡，男，1990年生，安徽省铜陵市人，汉族，南京农业大学食品科技学院生物工程专业在读，主要师从动物疾病模型研究。 stshiyuheng@126.com

Gene knockout mouse models for disease study

Shi Yu-heng   

  1. College of Food Science & Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China
  • Received:2011-06-01 Revised:2011-07-05 Online:2011-08-13 Published:2011-08-13
  • About author:Shi Yu-heng, College of Food Science & Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China stshiyuheng@126. com

PDF

778

被引次数

8

摘要：

背景：人类很多疾病是由基因决定的，构建相应的基因敲除动物模型对疾病的发病机制和治疗研究具有重要的意义。
目的：探讨基因敲除小鼠模型在心血管、神经、骨骼、肝脏、视网膜等系统的疾病研究中的应用与发展前景。
方法：应用计算机检索CNKI 期刊全文数据库和PubMed数据库(1990-01/2009-12)与基因敲除小鼠模型相关的文献。检索词分别为“基因敲除小鼠；动脉粥样硬化；神经系统；骨质疏松；肝脏；视网膜”和“Gene knock-out; Mice Atherosclerosis; Nervous system; Osteoporosis; Liver; Retinal”。纳入具有原创性，论点论据可靠的以基因敲除小鼠模型评价的文献报道。排除重复研究或Meta分析类文章。
结果与结论：检索到文献75篇，按纳入及排除标准筛选后共纳入文献38篇。经分析得出以下结论：基因敲除小鼠广泛应用于人类疾病模型，目前已构建了数百个人类疾病的小鼠模型，包括心血管疾病、神经退化性疾病、糖尿病、癌症等小鼠模型。通过对基因敲除小鼠疾病模型的研究，可以找到相关疾病的潜在治疗靶点，为人类精确地研究基因与疾病的直接关系提供了可能。

关键词: 基因敲除, 小鼠, 动脉粥样硬化, 神经系统, 骨质疏松, 肝脏, 视网膜

Abstract:

BACKGROUND: Many diseases in human are determined by genes. Constructing corresponding gene knockout animal models is of significance for treatment of diseases and studying the underlying pathological mechanisms.
OBJECTIVE: To investigate the application and research progress of knockout mouse models in studying cardiovascular, neurological, bone, liver, retinal diseases.
METHODS: A computer-based retrieval was performed to search manuscripts regarding knockout mouse models in CNKI and PubMed published between January 1990 and December 2009 with the key words “knockout mouse, atherosclerosis, nervous system, osteoporosis, liver, retina” in Chinese and English language. Manuscripts evaluating knockout mouse models with original and reliable evidence were included and those with repetitive contents or Meta analysis papers were excluded.
RESULTS AND CONCLUSION: Total 75 manuscripts were retrieved, and according to inclusion and exclusion criteria, 38 manuscripts were included in the final analysis. Results showed that knockout mouse models are widely used for study diseases in human. At present, several hundred of mouse models have been established for studying diseases in human, including cardiovascular disease, neurodegenerative diseases, diabetes mellitus, and caner. Studying knockout mouse models can acquire the therapeutic target of related diseases, which provide possibilities for precisely studying the direct relationship between gene and diseases.

中图分类号: