中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6199-6202.doi: 10.3969/j.issn.1673-8225.2011.33.029

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

黄精多糖对骨质疏松性骨折大鼠骨代谢因子的影响

曾高峰1，张志勇1，鲁  力1，肖德强1，宗少晖2，熊春翔3，赵玉玺3   

  1. 广西医科大学，1公共卫生学院，3研究生学院，广西壮族自治区南宁市 530021； 2广西医科大学第一附属医院脊柱骨病外科，广西壮族自治区南宁市 530021
  • 收稿日期:2011-02-14 修回日期:2011-03-19 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 张志勇，教授，主要从事环境职业与营养卫生方面的研究。广西医科大学公共卫生学院，广西壮族自治区南宁市 530021
  • 作者简介:曾高峰☆，女，1975年生，广西壮族自治区南宁市人，壮族，医学博士，副教授，主要从事营养与疾病方面的研究。 fengfeng_388@ 126.com
  • 基金资助:

    课题受广西教育厅科研基金(302204)和广西医科大学博士后流动站科研基金(308084)资助。

Effect of polygonatum polysaccharide on bone metabolism cytokines in osteoporotic fracture rats

Zeng Gao-feng1, Zhang Zhi-yong1, Lu Li1, Xiao De-qiang1, Zong Shao-hui2, Xiong Chun-xiang3, Zhao Yu-xi3   

  1. 1School of Public Health, 3Post-graduate School, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2Department of Spinal Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-02-14 Revised:2011-03-19 Online:2011-08-13 Published:2011-08-13
  • Contact: Zhang Zhi-yong, Professor, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Zeng Gao-feng☆, Doctor, Associate professor, School of Public Health, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China fengfeng_388@126. com
  • Supported by:

    the Scientific Research Foundation of Education Department of Guangxi, No. 302204*; the Science Foundation for Postdoctoral Station of Guangxi Medical University, No. 308084*

PDF

337

被引次数

20

摘要：

背景：骨质疏松性骨折主要原因是雌激素减少所形成的高转换型骨质疏松，采用雌激素虽能够有效地治疗，但长期雌激素替代治疗会引发诸多疾病。
目的：观察黄精多糖对骨质疏松性骨折大鼠骨代谢因子的影响。
方法：6月龄雌性SD大鼠，随机分为对照组(n=8)和骨质疏松性骨折组(n=36)。骨质疏松性骨折组行卵巢切除后建立骨质疏松性骨折动物模型，再以0.066 mg/kg雌激素，400，200，100 mg/kg 黄精多糖进行灌胃，2 d/次，连续35 d。采用Elisa方法测量骨钙素、抗酒石酸酸性磷酸酶的表达。
结果与结论：骨质疏松性骨折大鼠的骨钙素、抗酒石酸酸性磷酸酶的阳性表达活性升高，给予黄精多糖干预治疗后，高剂量的黄精多糖使骨钙素、抗酒石酸酸性磷酸酶的阳性表达活性下降，而在中、低剂量组干预效果不显著。说明高剂量黄精多糖对骨质疏松性骨折大鼠具有降低骨钙素、抗酒石酸酸性磷酸酶的阳性表达活性的作用，促进骨折愈合的作用。

关键词: 黄精多糖, 骨质疏松性骨折, 骨钙素, 抗酒石酸酸性磷酸酶, 中药单体

Abstract:

BACKGROUND: Onset of osteoporotic fracture is mainly resulted from reduction of estrogen that forms high-turnover osteoporosis, though this phenomenon can be treated effectively using estrogen, the long-term estrogen replacement therapy can cause many diseases.
OBJECTIVE: To investigate the effect of polygonatum polysaccharide (PSP) on the cytokines of bone metabolism in the osteoporotic fracture rats.
METHODS: Female, 6-month aged SD rats were divided into the control (n=8) and osteoporotic fracture (n=36) groups. After the success model preparation, rats in the osteoporotic fracture group were randomly treated by 0.066 mg/kg estrogen, 400, 200 or 100 mg/kg PSP by intragastric administration, twice per day, for 35 successive days. The expressions of bone gla protein (BGP) and tartrate-resistant acid phosphatase (TRAP) were detected by ELISA.
RESULTS AND CONCLUSION: BGP and TRAP levels were significantly increased in osteoporotic fracture rats. After PSP treatment, the BGP and TRAP levels were significantly decreased in the high dose PSP group. But intervention was not significant in medium and low dose PSP groups. It suggests that PSP can decrease BGP and TRAP expressions in the osteoporotic fracture rats. PSP can promote union of fracture.

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