中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6187-6190.doi: 10.3969/j.issn.1673-8225.2011.33.026

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

小鼠肾小管发育过程中纤维粘连蛋白与整合素α5的表达

阎丽菁1，田  鹤2，郭  敏2   

  1. 1辽宁医学院显微镜中心，辽宁省锦州市  121001
    2辽宁医学院组织学与胚胎学教研室，辽宁省锦州市  121001
  • 收稿日期:2011-02-19 修回日期:2011-06-01 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 郭敏，博士，教授，硕士生导师，辽宁医学院组织学与胚胎学教研室，辽宁省锦州市 121001 mguo1118@ yahoo.com.cn
  • 作者简介:阎丽菁★，女，1974年生，辽宁省锦州市人，汉族，2007年辽宁医学院毕业，硕士，实验师，主要从事胚胎发育的研究。 yanlijing_88@yahoo.com.cn
  • 基金资助:

    辽宁省教育厅基金项目(编号：L20100274，L20100269)。

Fibronectin and integrin alpha 5 expression during the development of mouse renal tubule

Yan Li-jing1, Tian He2, Guo Min2   

  1. 1 Microscope Centre，Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
    2 Department of Histology and Embryology, Liaoning Medical University, Jinzhou  121001, Liaoning Province, China
  • Received:2011-02-19 Revised:2011-06-01 Online:2011-08-13 Published:2011-08-13
  • Contact: Guo Min, Doctor, Professor, Master’s supervisor, Department of Histology and Embryology, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China mguo1118@yahoo. com.cn
  • About author:Yan Li-jing★, Master, Experimentalist, Microscope Centre，Liaoning Medical University, Jinzhou 121001, Liaoning Province, China yanlijing_88@yahoo.com.cn
  • Supported by:

    Education Department Foundation Program of Liaoning Province, No. L20100274*, L20100269*

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摘要：

背景：纤维粘连蛋白-整合素α5相互作用影响肾小管发育的体内研究较少。
目的：观察小鼠肾小管发育过程中纤维粘连蛋白及其受体整合素α5的表达。
方法：选取胚龄(E)12，14，16，18 d和生后日龄(P)1，3，7，14，21，28，40 d的小鼠。
结果与结论：免疫组织化学染色结果显示纤维粘连蛋白于E12 d时即表达于输尿管芽的基底膜处，随后表达于发育各个时期的肾小管基膜；整合素α5于E12 d时开始表达，表达部位是肾小管的上皮细胞。体视学测量结果显示纤维粘连蛋白表达的面密度值和整合素α5表达的体密度值都随肾脏的发育逐渐增大。Western blot结果显示纤维粘连蛋白和整合素α5蛋白的水平都随肾脏的发育逐渐增加。可见纤维粘连蛋白和整合素α5在小鼠肾小管的发育和成熟过程中的表达具有一定的时空性，对肾小管的发育起一定的作用。

关键词: 肾小管, 纤维粘连蛋白, 整合素α5, 小鼠, 发育

Abstract:

BACKGROUND: There are few studies describing interaction of fibronectin and integrin α5 influencing the development of renal tubules. 
OBJECTIVE: To observe the expression of fibronectin and its receptor integrin α5 during the development of mouse renal tubules.
METHODS: Kunming mice at embryonic days 12, 14, 16, 18 and postnatal days 1, 3, 7, 14, 21, 28 and 40 were selected.
RESULTS AND CONCLUSION: Immunohistochemical staining showed that fibronectin expression was expressed in the basement membrane of ureteric bud at embryonic 12 days and then in the basement membrane of renal tubules at any stage.  Integrin α5 began to express at embryonic 12 days in the epithelial cells of the renal tubules. Stereological measurements showed that the surface density of fibronectin and the volume density of integrin α5 increased gradually with the nephrogenesis. Western blot analysis showed that firbonectin and integrin α5 contents increased gradually with kidney development. These results demonstrate that fibronectin and intergrin α5 expression has certain chronological and spatial sequence during the development of mouse renal tubules, which suggest that they play an important role in the development of renal tubules.

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