中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6182-6186.doi: 10.3969/j.issn.1673-8225.2011.33.025

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

骨骼肌细胞线粒体通透性转换孔及bcl-2和bax mRNA表达与运动

王冬梅1，漆正堂2，丁树哲2   

  1. 1泰山医学院运动人体科学系，山东省泰安市  271016
    2华东师范大学体育与健康学院，上海市  200241
  • 收稿日期:2011-02-09 修回日期:2011-03-31 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 丁树哲，博士，教授，博士生导师，华东师范大学体育与健康学院，上海市 200241 szding@tyxx. ecnu.edu.cn
  • 作者简介:王冬梅★，女，1973年生，山东省淄博市人，汉族，2008年华东师范大学毕业，硕士，讲师，主要从事运动人体科学方面的研究。 wangdm72@126.com

Effects of exercises on mitochondrial permeability transition pore, and bcl-2 and bax mRNA expression in rat skeletal muscle

Wang Dong-mei1, Qi Zheng-tang2, Ding Shu-zhe2   

  1. 1Department of Sport and Body Science, Taishan Medical College, Tai’an 271016, Shandong Province, China
    2College of Physical Education & Health, East China Normal University, Shanghai  200241, China
  • Received:2011-02-09 Revised:2011-03-31 Online:2011-08-13 Published:2011-08-13
  • Contact: Ding Shu-zhe, Doctor, Professor, Doctor’s supervisor, College of Physical Education & Health, East China Normal University, Shanghai 200241, China szding@tyxx.ecnu. edu.cn
  • About author:Wang Dong-mei★, Master, Lecturer, Department of Sport and Body Science, Taishan Medical College, Tai’an 271016, Shandong Province, China wangdm72@126. com

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被引次数

1

摘要：

背景：运动影响骨骼肌细胞的凋亡，而线粒体途径是介导细胞凋亡的一个重要途径。
目的：研究运动对大鼠骨骼肌线粒体通透性转换孔、凋亡调控基因bcl-2和bax表达的影响。
方法：将24只成年雄性SD大鼠随机分为3组：对照组正常饲养，6周游泳训练组进行6周的游泳训练，每周6次，一次性游泳力竭组于第6周进行一次力竭性游泳运动。应用紫外分光光度仪检测各组大鼠骨骼肌线粒体通透性转换孔的开放情况，应用RT-PCR测定大鼠骨骼肌bcl-2和bax mRNA的表达。
结果与结论：与对照组比较，6周游泳训练组大鼠骨骼肌线粒体通透性转换孔的开放程度变化不明显，bcl-2 mRNA的表达显著增加，bax mRNA的表达显著减少，bcl-2/bax mRNA比值显著增大(P < 0.01)。与对照组比较，一次性游泳力竭组大鼠骨骼肌线粒体通透性转换孔开放程度明显增加(P< 0.01)，bcl-2 mRNA的表达显著减少，bax mRNA的表达显著增加，bcl-2/bax mRNA比值显著减小(P< 0.01)。说明运动训练可通过改变线粒体通透性转换孔的开放、调节bcl-2/bax表达，调控骨骼肌细胞凋亡。

关键词: 运动, 骨骼肌, 线粒体通透性转换孔, bcl-2, bax

Abstract:

BACKGROUND: Exercises influence the apoptosis of skeletal muscle cells and mitochondrial pathway is one of important pathways mediating cellular apoptosis.
OBJECTIVE: To investigate the effects of exercises on mitochondrial permeability transition pore, and bcl-2 and bax mRNA expression in rat skeletal muscle.
METHODS: Twenty-four Sprague-Dawley male rats were randomly divided into three groups: the control group, 6-week swimming training group (six swimming trainings per week) and one-off exhaustive swimming group (one-off exhaustive swimming exercise). The opening state of mitochondrial permeability transition pore in skeletal muscle was examined with ultraviolet spectrophotometer, and the mRNA expression of bcl-2 and bax in rat skeletal muscle was determined by reverse transcription-polymerase chain reaction.
RESULTS AND CONCLUSION: 6-week swimming training resulted in significant increase of bcl-2 mRNA expression and significant decrease of bax mRNA expression, as well as significant increase of bcl-2/bax mRNA(P < 0.01), but the opening state of mitochondrial permeability transition pore was not altered obviously, compared with the control group. The opening state of mitochondrial permeability transition pore was significantly increased (P < 0.01), bcl-2 mRNA expression was significantly decreased, bax mRNA expression was significantly increased, bcl-2/bax mRNA expression was significantly decreased in the one-off exhaustive swimming group than in the control group (P  < 0.01). These findings suggest that exercise training can regulate skeletal muscle cell apoptosis by altering the opening stage of mitochondrial permeability transition pore and regulating bcl-2/bax mRNA expression in rat skeletal muscle.

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