中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6147-6151.doi: 10.3969/j.issn.1673-8225.2011.33.017

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

脊髓损伤模型大鼠神经修复与法舒地尔和RhoA基因沉默的干预

柳兴军1，王雷波1，陈子祥1，张  赛2   

  1. 1天津市海河医院脑系科，天津市  300350
    2武警医学院附属医院脑系科中心，天津市 300162
  • 收稿日期:2011-05-13 修回日期:2011-06-17 出版日期:2011-08-13 发布日期:2011-08-13
  • 作者简介:柳兴军，男，1971年生，主治医师，主要从事颅脑创伤的基础和临床研究。 liuxingjun246810@126.com

Effect of Fasudil and RhoA gene silencing on nerve repair in a rat model of spinal cord injury

Liu Xing-jun1, Wang Lei-bo1, Chen Zi-xiang1, Zhang Sai2   

  1. 1Department of Brain, Tianjin Haihe Hospital, Tianjin  300350, China
    2Department of Brain, the Affiliated Hospital of Medical College of the Chinese People’s Armed Police Forces, Tianjin  300162, China
  • Received:2011-05-13 Revised:2011-06-17 Online:2011-08-13 Published:2011-08-13
  • About author:Liu Xing-jun, Attending physician, Department of Brain, Tianjin Haihe Hospital, Tianjin 300350, China liuxingjun246810@ 126.com

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280

被引次数

5

摘要：

背景：研究认为Rho激酶可致使神经生长锥塌陷，对神经修复具有抑制作用。
目的：观察Rho激酶抑制剂法舒地尔及RNA干预介导的RhoA基因沉默对脊髓损伤大鼠在体神经损伤修复的作用。
方法：雄性SD大鼠60只半切法制成脊髓半横断模型，随机等分成对照组、法舒地尔组和RhoA siRNA组。法舒地尔组于腹腔注射10 mg/kg法舒地尔，2次/d，连续用药1周；RhoA siRNA干扰组将RhoA siRNA表达质粒注射于大鼠脊髓损伤区。
结果与结论：伤后4周，法舒地尔和RhoA siRNA组大鼠后肢运动功能均有明显恢复，可见少量神经轴索样结构，辣根过氧化物酶阳性神经纤维数增多(P < 0.05)，体感诱发电位的潜伏期明显缩短、波幅显著增强(P < 0.05)。提示大鼠脊髓损伤后给予Rho激酶抑制剂法舒地尔及RNA介导的RhoA基因沉默能够促进受损伤的脊髓神经功能恢复。

关键词: 法舒地尔, RhoA, RNA干扰, 脊髓损伤, 大鼠

Abstract:

BACKGROUND: Several studies have demonstrated that Rho kinase can lead to collapse of nerve growth cone and exhibits an inhibitory effect on nerve repair.
OBJECTIVE: To investigate the effect of Rho kinase inhibitor Fasudil and RNAi-mediated RhoA gene silencing on nerve repair in a rat model of spinal cord injury.
METHODS: Sixty male Sprague-Dawley rats were prepared into spinal cord hemisection and then were randomly divided into control, Fasudil and RhoA siRNA groups. The Fasudil group rats were intraperitoneally injected with 10 mg/kg Fasudil, twice a day, for successive 1 week. The RhoA siRNA group rats were injected with RhoA siRNA expression plasmid into spinal cord injury area.
RESULTS AND CONCLUSION: At 4 weeks after injection, the hind limb motor function of the Fasudil and RhoA siRNA group rats was obviously recovered, neuronal axon-like structure was observed, horseradish peroxidase-positive nerve fibers were increased (P < 0.05), somatosensory evoked potential latency was obviously shortened, and amplitude was significantly increased (P < 0.05). These results showed that after spinal cord injury, Rho kinase inhibitor Fasudil and RNAi-mediated RhoA gene silencing can promote the neurofunctional recovery of injured spinal cord.

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