中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6111-6114.doi: 10.3969/j.issn.1673-8225.2011.33.008

• 骨组织构建 bone tissue construction • 上一篇    下一篇

雌激素与葛根素组合治疗去卵巢大鼠骨症的最佳剂量

黄海玲，李  海，王金花，黎  飚，赵琼芝，刘  洁，解继胜   

  1. 右江民族医学院组织胚胎学教研室，广西壮族自治区百色市 533000
  • 收稿日期:2011-05-30 修回日期:2011-06-24 出版日期:2011-08-13 发布日期:2011-08-13
  • 作者简介:黄海玲★，女，1972年生，广西壮族自治区百色市人，汉族，2003年武汉大学毕业，硕士，副教授，主要从事雌激素及其受体在雌激素依赖性疾病中作用的研究。 yyhuanghailing@126.com
  • 基金资助:

    课题由广西自然科学基金(合同编号2010GXNSFA013268)和右江民族医学院科研课题基金(yk2009002)资助；课题名称：葛根素联合雌二醇对去卵巢大鼠骨组织和血钙、磷的影响。

Effects of optimal dose of estradiol combined with puerarin on bone in ovariectomized rats

Huang Hai-ling, Li Hai, Wang Jin-hua, Li Biao, Zhao Qiong-zhi, Liu Jie, Xie Ji-sheng   

  1. Department of Histology and Embryology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China
  • Received:2011-05-30 Revised:2011-06-24 Online:2011-08-13 Published:2011-08-13
  • About author:Huang Hai-ling★, Master, Associate professor, Department of Histology and Embryology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China yyhuanghailing@126.com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No.2010GXNSFA013268*; Scientific Research Program of Youjiang Medical College for Nationalities, No. yk2009002*

PDF

403

被引次数

10

摘要：

背景：小剂量的葛根素联合雌二醇对去卵巢大鼠骨质疏松症的治疗效果与单独使用较大剂量的葛根素或较大剂量的雌二醇效果相近。
目的：寻找治疗Ⅰ型骨质疏松症最佳的雌二醇和葛根素的剂量搭配。
方法：64只健康雌性大白鼠等分为假手术组、去卵巢模型组、葛根素-50组、雌二醇-200组、葛根素+雌二醇-5，50，100，150组。除假手术组外，其余大鼠均建立去卵巢动物模型。葛根素-50组大鼠皮下注射葛根素50 mg/kg，1次/d；雌二醇-200组大鼠皮下注射雌二醇200 μg/kg，2次/周；葛根素+雌二醇-5，50，100，150组大鼠皮下注射葛根素25 mg/kg，1次/d同时分别注射雌二醇5，50，100，150 μg/kg，2次/周。
结果与结论：去卵巢模型组大鼠骨密度和骨钙、骨磷水平明显低于假手术组(P < 0.05)，骨组织呈骨质疏松的病理改变；葛根素和/或雌二醇治疗10，20周后骨密度和骨钙、骨磷水平明显升高(P < 0.05)，其中葛根素+雌二醇-100组治疗去卵巢大鼠骨质疏松的效果最为明显，提示葛根素25 mg/kg，1次/d+雌二醇100 μg/kg，2次/周是治疗去卵巢大鼠骨质疏松的最佳搭配剂量。

关键词: 葛根素, 雌二醇, 去卵巢, 大鼠, 骨质疏松症, 剂量搭配

Abstract:

BACKGROUND: Low dose of puerarin combined with estradiol shows similar effects to high dose of puerarin or estradiol on treatment of osteoporosis in rats.
OBJECTIVE: To search the optimal doses for estradiol and puerarin for treatment of typeⅠosteoporosis.
METHODS: Sixty-four healthy female rats were randomly divided into eight experimental groups (n = 8): sham-operated, ovariectomized, puerarin-50, estradiol-200, puerarin+estradiol-5, 50, 10, 150. All rats were subjected to ovariectomy except those in the sham-operated group. Rats in the puerarin-50 group were subcutaneously injected with 50 mk/kg puerarin, once a day. Rats in the estradiol-200 group were subcutaneously injected with 200 μg/kg estradiol, twice a week. Rats in the puerarin+ estradiol-5, 50, 10, 150 groups were subcutaneously injected with 25 mg/kg puerarin, once a day and simultaneously injected with 5, 50, 10, 150 μg/kg estradiol, twice a week.
RESULTS AND CONCLUSION: Compared with sham-operated group, bone mineral density, bone calcium, and bone phosphorous of rats were significantly lower in the ovariectomized group (P < 0.05), and bone tissue presented with the pathological change of osteoporosis. After treatment with puerarin and/or estradiol for 10 and 20 weeks, bone mineral density, bone calcium, and bone phosphorous levels were significantly increased (P < 0.05), and puerarin+estradiol-100 group acquired the most obvious curative effects on osteoporosis. The results showed that 25 mg/kg puerarin (once a day) and 100 μg/kg estradiol (twice a day) is the best dose for treatment of osteoporosis in ovariectomized rats.

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