中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5991-5994.doi: 10.3969/j.issn.1673-8225.2011.32.023

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

脐血间充质干细胞静脉移植治疗新生鼠脑血肿继发脑水肿

颜小华1，陈启文1，徐  昕1，李延成2，严绍明3，黄振刚1，张丽娜1   

  1. 1南昌大学第一附属医院儿科，江西省南昌市  330006
    2江西省交通医院内科，江西省南昌市   330003
    3 江西省永丰县人民医院儿科，江西省吉安市  331500
  • 收稿日期:2011-02-21 修回日期:2011-05-22 出版日期:2011-08-06 发布日期:2011-08-06
  • 作者简介:颜小华☆，男，1966年生，江西省萍乡市人，汉族，2004年中南大学湘雅医学院毕业，博士，副主任医师，副教授，主要从事小儿神经性疾病的研究。 yanxiaohua456@yahoo.com.cn 并列第一作者：陈启文，男，1963年生，江西省九江市人，汉族，1987年江西医学院毕业，副主任医师、副教授，主要从事小儿血液病的干细胞移植研究。xymu2001phd@yahoo.com
  • 基金资助:

    江西省科技厅支撑计划课题基金资助项目(赣科发计字[2009]247号)。

Effects of intravenous transplantation of umbilical blood mesenchymal stem cells on cerebral edema secondary to hematoma in neonatal rats

Yan Xiao-hua1, Chen Qi-wen1, Xu Xin1, Li Yan-cheng2, Yan Shao-ming3, Huang Zhen-gang1, Zhang Li-na1   

  1. 1Department of Pediatrics, the First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
    2Department of Internal Medicine, Jiaotong Hospital of Jiangxi Province, Nanchang  330003, Jiangxi Province, China
    3Department of Pediatrics, Yongfeng County People’s Hospital, Ji’an  331500, Jiangxi Province, China
  • Received:2011-02-21 Revised:2011-05-22 Online:2011-08-06 Published:2011-08-06
  • About author:Yan Xiao-hua☆, Doctor, Associate chief physician, Associate professor, Department of Pediatrics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China yanxiaohua456@yahoo.com.cn Chen Qi-wen, Associate chief physician, Associate professor, Department of Pediatrics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China xymu2001phd@yahoo.com
  • Supported by:

    Support Program Research Fund of Jiangxi Science and Technology Bureau, No. [2009]247*

PDF

272

被引次数

3

摘要：

背景：脐血间充质干细胞移植治疗新生动物脑血肿继发脑水肿的研究少见报道。
目的：探讨脐血间充质干细胞静脉移植治疗新生鼠实验性脑血肿继发脑水肿的可行性。
方法: 健康新生7 d SD大鼠分为未进行干预的空白对照组、仅制作脑出血模型的脑血肿对照组和制作脑出血模型后给予脐血间充质干细胞治疗的移植组。3组大鼠均于移植后1周处死，提取脑组织进行指标检测。
结果与结论：脑血肿形成后，脑系数和脑组织含水量均明显增加，脐血间充质干细胞移植组明显小于脑血肿对照组(P < 0.01)，与空白对照组比较差异无显著性意义(P > 0.05)。移植1周后，各组鼠死亡率无显著差异，均未见植入反应和其他任何负作用。提示，脐血间充质干细胞静脉移植治疗能有效减轻实验性脑血肿继发的脑水肿，对其具有明显的保护作用。其机制可能与脐血间充质干细胞能有效地透过血脑屏障，在病灶脑组织周围迁移、扩散、整合，从而在病灶脑组织存在并分泌大量保护性细胞因子有关。

关键词: 脐血间充质干细胞, 细胞移植, 实验性脑血肿, 脑水肿, 新生大鼠

Abstract:

BACKGROUND: There are few reports about human umbilical cord blood mesenchymal stem cells (HUCBMSCs) for cerebral edema secondary to hematoma in neonatal animals.
OBJECTIVE: To study the possibility of intravenous transplantation of HUCBMSCs in the treatment of cerebral edema secondary to hematoma in neonatal rats.
METHODS: Neonatal rats aged 7 days were randomly assigned into three groups: normal control, model group and MSCs transplantation group. One week after transplantation, the rats in each group were randomly killed to extract brain tissues which were used pathomorphology observation and index measurement.
RESULTS AND CONCLUSION: Both brain coefficient and water content of brain tissue were significantly lower in the MSCs group than the model group (P < 0.01). However, there was no difference between MSCs group and control group (P > 0.05). There was no difference in the death rate of rats in each group 1 week after the implantation. The brain damage to most of the rats in this group was not serious but only slight by chance, close to the normal at the one week after implantation. HUCBMSCs transplantation could effectively decrease the brain edema and ameliorate the damaged brain tissue. One week after transplantation, the MSCs appeared in the ischemic damaged brain tissue as a large number of MSCs could cross the blood brain barrier to distribute and scatter around the disease focus integrated with host brain tissue without apparent threshold.

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