中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5967-5970.doi: 10.3969/j.issn.1673-8225.2011.32.017

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

骨形态发生蛋白2对14 d胎鼠端脑神经干细胞分化为胆碱能神经元的影响

梁  军1，李明秋2，赵富生1，董建将1，李月珍1   

  1. 牡丹江医学院，1组织学与胚胎学教研室，2解剖教研室，黑龙江省牡丹江市 157011
  • 收稿日期:2011-02-08 修回日期:2011-03-10 出版日期:2011-08-06 发布日期:2011-08-06
  • 作者简介:梁军☆，男，1972年生，黑龙江省牡丹江市人，朝鲜族，吉林大学白求恩医学部在读博士，副教授，主要从事神经系统疾病治疗的基础研究。 mdjzplj@126.com
  • 基金资助:

    牡丹江市科学技术计划项目(G2010s0020)，课题名称“基底前脑胆碱能神经元发生的研究”。

Effects of bone morphogenetic proteins 2 on neural stem cells of 14-day-old fetal rat telencephalon differentiating into cholinergic neurons

Liang Jun1, Li Ming-qiu2, Zhao Fu-sheng1, Dong Jian-jiang1, Li Yue-zhen1   

  1. 1Department of Histology and Embryology, 2Department of Anatomy, Mudanjiang Medical College, Mudanjiang  157011, Heilongjiang Province, China
  • Received:2011-02-08 Revised:2011-03-10 Online:2011-08-06 Published:2011-08-06
  • About author:Liang Jun☆, Studying for doctorate, Associate professor, Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang Province, China mdjzplj@126.com
  • Supported by:

    the Sci-Technique Project of Mudanjiang, No. G2010s0020*

PDF

397

被引次数

3

摘要：

背景：骨形态发生蛋白2可能是参与胆碱能神经元前体细胞分化的细胞外调控因子。
目的：观察骨形态发生蛋白2在孕14 d胎鼠端脑神经干细胞诱导成胆碱能神经元过程中的作用。
方法：取孕14 d胎鼠端脑，用含EDTA的胰酶和Ⅰ型胶原酶消化，无血清培养基培养细胞，种植于涂有多聚赖氨酸的培养板，细胞原代培养24 h后半量换液，加入10 μg/L骨形态发生蛋白2继续培养。
结果与结论：胶原酶消化得到的神经干细胞呈单层贴壁生长；Nestin免疫荧光鉴定细胞为阳性，获取的神经干细胞纯度大于99%；ChAT免疫荧光鉴定骨形态发生蛋白2可以将孕14 d胎鼠端脑神经干细胞诱导成胆碱能神经元，细胞纯度大于97%。

关键词: 骨形态发生蛋白2, 神经干细胞, 胆碱能神经元, 端脑, 胎鼠

Abstract:

BACKGROUND: Bone morphogenetic protein-2 (BMP2) may be an extracellular regulatory factor involved in cholinergic differentiation of neuronal precursor cells.
OBJECTIVE: To explore the effects of BMP2 on neural stem cells of 14-day-old fetus rat telencephalons induced into cholinergic neurons.
METHODS: Fetus telencephalons of 14-day-old SD rats were isolated, and tissues were divisively treated with collagenase typeⅠcontained EDTA following trituration. The cells were raised in polylysine culture plates with serum-free medium. Primary culture medium was changed half after 24 hours, and 10 μg/L BMP2 was added.
RESULTS AND CONCLUSION: The adherent monoculture neural stem cells could been obtained using collagenase. The Nestin positve cells were obtained, and the purity was over 99%. After induced by BMP2, the ChAT positve cells were obtained, and the purity was over 97%. BMP2 can induce neural stem cells of 14-day-old fetus rat telencephalons into cholinergic neurons.

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