中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5971-5974.doi: 10.3969/j.issn.1673-8225.2011.32.018

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

骨形态发生蛋白9诱导骨骼肌源性干细胞的成骨分化

李  翔，林春阳，邱北溟，陈  亮，邓忠良   

  1. 重庆医科大学附属第二医院骨科，重庆市  400010
  • 收稿日期:2011-02-19 修回日期:2011-03-28 出版日期:2011-08-06 发布日期:2011-08-06
  • 通讯作者: 邓忠良，博士，教授，主任医师，重庆医科大学附属第二医院骨科，重庆市 400010 Deng7586@gmail.com 通讯作者：陈亮，博士，讲师，主治医师，重庆医科大学附属第二医院骨科，重庆市400010 Beneal.chen@gmail.com
  • 作者简介:李翔★，男，1983年生，四川省泸州市人，汉族，重庆医科大学在读硕士，主要从事成骨干细胞研究。 lixiang1200@gmail.com
  • 基金资助:

    重庆市科委基金(CSTC，2008BB5078) ，国家自然科学基金(30772211)，国家自然科学基金(31000434)。

Bone morphogenetic protein 9 induces skeletal muscle-derived stem cells differentiating into osteoblasts

Li Xiang, Lin Chun-yang, Qiu Bei-ming, Chen Liang, Deng Zhong-liang   

  1. Department of Orthopedics, Second Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
  • Received:2011-02-19 Revised:2011-03-28 Online:2011-08-06 Published:2011-08-06
  • Contact: Deng Zhong-liang, Doctor, Professor, Chief physician, Department of Orthopedics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China Deng7586@gmail.com Correspondence to: Chen Liang, Doctor, Lecturer, Attending physician, Department of Orthopedics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China Beneal.chen@gmail.com
  • About author:Li Xiang★, Studying for master’s degree, Department of Orthopedics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China lixiang1200@gmail.com
  • Supported by:

    the Fund of Chongqing Science and Technology Committee, No. 2008BB5078*; the National Natural Science Foundation of China, No. 30772211*, 31000434*

PDF

434

被引次数

10

摘要：

背景：已有实验报道部分骨形态发生蛋白能够诱导间充质干细胞等向成骨细胞的分化。
目的：评估骨形态发生蛋白9定向诱导骨骼肌源性干细胞成骨分化的能力。
方法：采用Ⅰ型胶原酶和胰蛋白酶二步消化法提取兔骨骼肌源性干细胞，用重组腺病毒的方法将骨形态发生蛋白9导入细胞，通过碱性磷酸酶定量测定、钙盐沉积实验观察骨形态发生蛋白9对于骨骼肌源性干细胞成骨分化的定向诱导作用。
结果与结论：成功分离培养兔骨骼肌源性干细胞，免疫细胞化学染色80%以上的细胞呈Sca-1 阳性。骨形态发生蛋白9能诱导骨骼肌源性干细胞碱性磷酸酶的表达，且能够促进细胞的钙盐沉积。提示，骨形态发生蛋白9具有定向诱导骨骼肌源性干细胞分化成骨的能力。

关键词: 骨骼肌源性干细胞, 骨形态发生蛋白9, 成骨细胞, 细胞培养, 碱性磷酸酶

Abstract:

BACKGROUND: Previous studies found that some bone morphogenetic proteins can induce mesenchymal stem cells to differentiate into osteoblasts.
OBJECTIVE: To assess the capacity of bone morphogenetic protein 9 to induce the differentiation of skeletal muscle-derived stem cells into osteoblasts.
METHODS: Rabbit skeletal muscle-derived stem cells were obtained by the two-step method of collagenase-Ⅰ and trypsin, and transfected by bone morphogenetic protein 9 through recombinant adenoviruses. Then, the effect of bone morphogenetic protein 9 on the differentiation of skeletal muscle-derived stem cells into osteoblasts was observed by testing the alkaline phosphatase quantitation and calcium deposition.
RESULTS AND CONCLUSION: Muscle-derived stem cells were successfully isolated from the rabbit and over 80% of muscle-derived stem cells showed Sca-1 positive immunoreactivity. Bone morphogenetic protein 9 induced alkaline phosphatase activities in skeletal muscle-derived stem cells and promoted calcium deposition of the cells. From the above, bone morphogenetic protein 9 has the capability to induce the differentiation of skeletal muscle-derived stem cells into osteoblasts．

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