中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5813-5816.doi: 10.3969/j.issn.1673-8225.2011.31.027

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

自制多脏器保存液对大鼠睾丸一氧化氮合酶的影响

孙  雯，张金萍，揭  芳，徐超超，金荣盘，范奇琪，钱申伦，叶  波   

  1. 绍兴文理学院医学院临床医学系，浙江省绍兴市  312000
  • 收稿日期:2011-04-14 修回日期:2011-06-09 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 张金萍，教授，绍兴文理学院医学院基础医学部，浙江省绍兴市 312000 pjzxuan@126. com
  • 作者简介:孙雯，女，1987年生，天津市人，汉族，绍兴文理学院本科在读，主要从事临床医学专业。 911841162@ 163.com
  • 基金资助:

    2010年浙江省大学生科技创新活动计划(新苗人才计划)项目(2010R426002)；2009年绍兴市大学生科技创新项目(200912)；绍兴文理学院第一批校级优秀教学团队建设成果(20097) 。

Effects of self-made multi-organ preservation solution on nitric oxide synthase in cryopreserved rat testicle

Sun Wen, Zhang Jin-ping, Jie Fang, Xu Chao-chao, Jin Rong-pan, Fan Qi-qi, Qian Shen-lun, Ye Bo   

  1. Department of Clinical Medicine, School of Medicine, Shaoxing University, Shaoxing  312000, Zhejiang Province, China
  • Received:2011-04-14 Revised:2011-06-09 Online:2011-07-30 Published:2011-07-30
  • Contact: Zhang Jin-ping, Professor, Department of Clinical Medicine, School of Medicine, Shaoxing University, Shaoxing 312000, Zhejiang Province, China pjzxuan@126.com
  • About author:Sun Wen, Department of Clinical Medicine, School of Medicine, Shaoxing University, Shaoxing 312000, Zhejiang Province, China 911841162@163 com
  • Supported by:

    Science and Technology Innovation Program of College and University Students of Zhejiang Province in 2010, No.2010R426002*; Science and Technology Innovation Program of College and University Students of Shaoxing City in 2009, No.200912*; a grant from the First Batch Collage-level Excellent Teaching Team Construction Achievement Foundation of Shaoxing University, No.20097*

PDF

295

被引次数

1

摘要：

背景：在睾丸移植过程中，低温保存和缺血可导致睾丸产生氧自由基而损伤睾丸组织。
目的：观察自制多脏器保存液对低温保存大鼠睾丸一氧化氮合酶的影响。
方法：采用自制多脏器保存液和UW液低温保存大鼠睾丸，分别于保存24，48，72 h时切取睾丸，测定睾丸组织内的总抗氧化能力和一氧化氮合酶活性。
结果与结论：自制多器官保存液低温保存各时点大鼠睾丸一氧化氮合酶活性和总抗氧化能力与UW液组比较差异均无显著性意义(P > 0.05)。表明自制多脏器保存液能明显减轻低温保存大鼠睾丸氧自由基损伤，其作用与经典的美国威斯康星大学UW保存液基本相当。

关键词: 多脏器保存液, 低温保存, UW保存液, 睾丸, 移植, 一氧化氮合酶 

Abstract:

BACKGROUND: During testis transplantation, cryopreservation and ischemia can lead to production of oxygen free radicals by testis, which damages testis tissue.
OBJECTIVE: To investigate the effects of self-made multi-organ preservation solution on nitric oxide synthase (NOS) in cryopreserved rat testicle.
METHODS: Rat testicle was cryopreserved by self-made multi-organ preservation solution and UW solution. At 24, 48, and 72 hours after cryopreservation, testicular tissue was taken to determine total antioxidant capacity and NOS activity.
RESULTS AND CONCLUSION: There was no significant difference in total antioxidant capacity and NOS activity in rat testicular tissue cryopreserved in the self-made multi-organ preservation solution versus UW solution (P > 0.05). These findings suggest that self-made multi-organ preservation solution can obviously reduce oxygen free radical damage in cryopreserved rat testicular tissue and its effects are equivalent to US solution. 

中图分类号: