中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (25): 4647-4650.doi: 10.3969/j.issn.1673-8225.2011.25.021

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

聚甲基丙烯酸甲酯颗粒对人滑膜细胞核因子κB受体激动剂配体/骨保护蛋白表达的影响

熊  奡1，苏志雄2，马泽涛1，康  斌1，辛  风1   

  1. 1深圳市北京大学深圳医院骨关节科，广东省深圳市 518036
    2清华大学第一附属医院骨科，北京市100016
  • 收稿日期:2010-12-29 修回日期:2011-02-15 出版日期:2011-06-18 发布日期:2014-01-10
  • 作者简介:熊奡，男，1976年生，湖北省武汉市人，汉族，2000年华中科技大学毕业，主治医师，主要从事关节外科研究。 xiongao@21cn.com

Effects of polymethyl methacrylate particles on human synoviocytes receptor activator of nuclear factor kappa B ligand / osteoprotegerin expression

Xiong Ao1, Su Zhi-xiong2, Ma Ze-tao1, Kang Bin1, Xin Feng1   

  1. 1Department of Orthopedics, Shenzhen Hospital of Peking University, Shenzhen  518036, Guangdong Province, China
    2Department of Orthopedics, First Affiliated Hospital of Tsinghua University, Beijing  100016, China
  • Received:2010-12-29 Revised:2011-02-15 Online:2011-06-18 Published:2014-01-10
  • About author:Xiong Ao, Attending physician, Department of Orthopedics, Shenzhen Hospital of Peking University, Shenzhen 518036, Guangdong Province, China xiongao@21cn.com

PDF

302

摘要：

背景：多种因子可以影响骨保护蛋白/核因子κB受体激动剂配体/核因子κB受体激动剂(Osteoprotegerin / receptor activator of nuclear factor-kappaB/ligand of receptor-activator of nuclear factor-kappaB，OPG/RANKL/RANK)系统的表达影响骨代谢，那么松动假体周围的骨水泥颗粒是否也通过影响其代谢参与假体松动过程？
目的：观察聚甲基丙烯酸甲酯颗粒对人滑膜细胞RANKL/OPG表达的影响。
方法：体外培养人滑膜细胞，在滑膜细胞培养体系中分别加入质量浓度为0(空白对照)，0.2，1，10 g/L的聚甲基丙烯酸甲酯骨水泥颗粒，采用荧光定量PCR检测上述各浓度PMMA颗粒作用不同时间后滑膜细胞内RANKL、OPG的表达量及其比值。
结果与结论：与空白对照组相比，各实验组滑膜细胞内RANKL、OPG的表达量均有下降；随着与聚甲基丙烯酸甲酯颗粒共培养时间延长，各实验组滑膜细胞内RANKL、OPG表达均有下降趋势，而RANKL/OPG表达比值无明显变化。说明聚甲基丙烯酸甲酯颗粒在对滑膜细胞产生生物学反应时并不干扰假体周围骨代谢的动态平衡，并未直接参与人工关节假体的无菌性松动。

关键词: 聚甲基丙烯酸甲酯(PMMA), 骨水泥, 滑膜, 核因子&kappa, B受体激活因子配体(RANKL), 骨保护蛋白(OPG)

Abstract:

BACKGROUND: Various factors can influence bone metabolism through expression of osteoprotegerin/receptor activator of nuclear factor-κB/ligand of receptor-activator of nuclear factor-κB (OPG/RANKL/RANK) system. Whether bone cement particles also affects bone metabolic balance around prosthesis via OPG/RANKL/RANK expression in aseptic loosening remains poorly understood.
OBJECTIVE: To investigate the effects of polymethyl methacrylate (PMMA) particles on the expression of RANKL/OPG in human synoviocytes.
METHODS: In vitro, human synovial cells were cultured with different concentrations of PMMA particles (0, 0.2, 1 and 10 g/L). The expression of RANKL, OPG and their ratio of each group was detected by fluorescence quantitative PCR at different time points.
RESULTS AND CONCLUSION: Compared with the control group, the expression of RANKL and OPG in each experimental group was significantly down-regulated with time prolonged. While, there was no significant difference in the ratios of RANKL/OPG expression. Synoviocytes biologically reacting to PMMA particles had no interference on the dynamic balance of bone metabolism around prosthesis, thus play no obvious role in the aseptic loosening of artificial prosthesis.

中图分类号: