中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2716-2720.doi: 10.3969/j.issn.1673-8225.2011.15.014

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

一种新的大鼠门静脉动脉化模型

吴均政1，江  艺1，张小进2，吕立志2   

  1. 1福建医科大学福总临床医学院，福建省福州市  350025
    2解放军南京军区福州总医院肝胆外科，福建省福州市  350025
  • 收稿日期:2010-10-19 修回日期:2010-12-19 出版日期:2011-04-09 发布日期:2013-11-06
  • 通讯作者: 江艺，博士，教授，博士生导师，福建医科大学福总临床医学院，福建省福州市 350025 jiangyi183@yahoo.com.cn
  • 作者简介:吴均政★，男，1985年生，汉族，福建省泉州市人，福建区科大学在读硕士，主要从事肝胆外科、肝脏移植方面的研究。 dr.wujunzheng@gmail.com
  • 基金资助:

    南京军区医药卫生科研基金项目(08MA104)，课题名称：干细胞联合肝移植的临床和实验研究。

Establishment of a new rat model of portal vein arterialization

Wu Jun-zheng1, Jiang Yi1, Zhang Xiao-jin2, Lü Li-zhi2   

  1. 1School of Clinical Medicine, Fuzhou General Hospital of Nanjing Military Region, Fujian Medical University, Fuzhou  350025, Fujian Province, China
    2Department of Hepatobiliary Surgery, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou  350025, Fujian Province, China
  • Received:2010-10-19 Revised:2010-12-19 Online:2011-04-09 Published:2013-11-06
  • Contact: Jiang Yi, Doctor, Professor, Doctoral supervisor, School of Clinical Medicine, Fuzhou General Hospital of Nanjing Military Region, Fujian Medical University, Fuzhou 350025, Fujian Province, China jiangyi183@yahoo. com.cn
  • About author:Wu Jun-zheng★, Studying for master’s degree, School of Clinical Medicine, Fuzhou General Hospital of Nanjing Military Region, Fujian Medical University, Fuzhou 350025, Fujian Province, China dr.wujunzheng@ gmail.com
  • Supported by:

    the Scientific Research Foundation for Medical Science of Nanjing Military Region, China, No. 08MA104*

PDF

421

被引次数

10

摘要：

背景：目前文献报道的大鼠门静脉动脉化模型有缝合法或支架法，2种方法在血管通畅率及操作便利性上均不太理想。
目的：建立稳定的操作简便的大鼠门静脉动脉化动物模型。
方法：采用同种异体血管套入式缝合及袖套法建立大鼠门静脉动脉化模型40例。切除左肾，将左肾动脉与门静脉残端通过同种异体血管连接，左肾静脉借助袖套与肠系膜上静脉连接。另取10只大鼠作为假手术组。术后观察大鼠的体质量变化和存活情况，2周行磁共振血管成像，1个月检测大鼠肝功能，2个月处死大鼠探查门静脉血流通畅情况并观察肝脏的病理学改变。
结果与结论：造模过程中有1只大鼠死亡，其余39只均存活至术后2个月，造模成功率为98%(39/40)。术后1个月，模型组与假手术组大鼠体质量，血清白蛋白、谷丙转氨酶、碱性磷酸酶和胆碱酯酶差异均无显著性意义(P > 0.05)。术后2个月，模型组门静脉通畅率为95%(37/39)。磁共振血管成像及病理结果均未见明显异常。提示，利用同种异体血管套入式缝合及袖套法建立的大鼠门静脉动脉化模型操作简便、省时、成功率高，是一种可行、稳定、可靠、可重复性强的方法。

关键词: 门静脉动脉化, 同种异体血管, 套入式缝合, 袖套法, 动物模型, 大鼠, 血管组织工程

Abstract:

BACKGROUND: At present literature reported that portal vein arterializations model of rats with suture method or stent method in the blood patency and operating simplicity are not perfect.
OBJECTIVE: To establish a stable and simple model of portal vein arterialization in rats. 
METHODS: A total of 40 cases of portal vein arterializations model in rats were established by the allogeneic vascular embolia suture method and cuff method. Left kidney was removed. Left renal artery was connected with portal vein stump by allogeneic vascular; the left renal vein was connected with superior mesenteric vein by cuff method. Another 10 rats served as sham operation group. The body weight and survival rate were observed after surgery. The rats were executed magnetic resonance imaging (MRI) after 2 weeks. Hepatic function of rats was detected at 1 month after surgery. Two months after surgery, all rats were killed to probe portal vein patency, and hepatic pathological changes were observed.
RESULTS AND CONCLUSION: One rat dead during modeling, the remaining 39 rats survived after two months. The successful rate of operation was 97.5% (39/40). At one month after surgery, there was no significant difference in body weight, albumin, alanine aminotransferase, alkaline phosphatase, and cholinesterase between model group and sham operation group (P > 0.05). Two months after surgery, the patency rate of portal vein was 94.9% (37/39) in model group. Both MRI and pathological results showed no obvious abnormalities. Based on the allogeneic vascular embolia suture method and cuff method, the rat model of portal vein arterialization have properties in simple operation, time-saving and high successful rate, which is a viable, stable, reliable and reproducible method.

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