中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2721-2724.doi: 10.3969/j.issn.1673-8225.2011.15.015

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

缺血心肌梗死模型大鼠中等强度运动后梗死面积的变化

李  丹1,2,3，赵宝寅1,2,3，李  磊1,2,3，钟嘉泳4，张志鹏4，陈夷林1,2,3，沈晓涛1,2,3,4，李艳梅1,2,3，禹艳红1,5，蔡冬青1,2,3   

  1. 暨南大学，1再生医学教育部重点实验室，2再生医学香港中文大学-暨南大学联合实验室，3 科技部及广东省科技厅国际科技合作基地，4生物医学工程系，5生殖免疫研究所, 广东省广州市  510632
  • 收稿日期:2011-01-04 修回日期:2011-03-05 出版日期:2011-04-09 发布日期:2013-11-06
  • 通讯作者: 蔡冬青，教授，博士生导师，暨南大学，再生医学教育部重点实验室，再生医学香港中文大学-暨南大学联合实验室，科技部及广东省科技厅国际科技合作基地，广东省广州市 510632 tdongbme@jnu. edu.cn
  • 作者简介:李丹★，女，1986年生，湖北省潜江市人，汉族，暨南大学再生医学教育部重点实验室在读硕士，主要从事缺血心肌再生方面的研究。 quanliyifu66@ 163.com
  • 基金资助:

    863项目(2007AA02Z105)；国家自然科学基金(30770886，30570369，30340038，30973158)；广东省自然科学基金重点项目(04105826)；广东省科技攻关项目(2004B30601007)；广东省国际合作项目(2009B050900007)；中央高校基本科研业务费专项资金(21609408)。

Ischemic area changes following moderate training in rat models of ischemic heart

Li Dan1,2,3, Zhao Bao-yin1,2,3, Li Lei1,2,3, Zhong Jia-yong4, Zhang Zhi-peng4, Chen Yi-lin1,2,3, Shen Xiao-tao1,2,3,4, Li Yan-mei1,2,3, Yu Yan-hong1,5, Cai Dong-qing1,2,3   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3The International Base of Collaboration for Science & Technology (Jinan University), the Ministry of Science and Technology & Guangdong Province Scinece and Technology Bureau, 4Department of Biomedical Engineering, 5Institute of Reproductive Immunology, College of Life Science and Technology, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2011-01-04 Revised:2011-03-05 Online:2011-04-09 Published:2013-11-06
  • Contact: Cai Dong-qing, Professor, Doctoral supervisor, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3The International Base of Collaboration for Science & Technology (Jinan University), the Ministry of Science and Technology & Guangdong Province Scinece and Technology Bureau, Jinan University, Guangzhou 510632, Guangdong Province, China tdongbme@jnu.edu.cn
  • About author:Li Dan★, Studying for master’s degree, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3The International Base of Collaboration for Science & Technology (Jinan University), The Ministry of Science and Technology & Guangdong Province Scinece and Technology Bureau, Jinan University, Guangzhou 510632, Guangdong Province, China quanliyifu66@163.com
  • Supported by:

    the National 863 Program of China, No. 2007AA02Z105*; the National Natural Science Foundation of China, No. 30770886*, 30570369*, 30340038*, 30973158*; the Key Program of Guangdong Natural Science Foundation, No. 04105826*; Guangdong Grant for Science and Technology Development, No. 2004B30601007*; International Collaborated Grant of Guangdong, No. 2009B050900007*; the Fundamental Research Funds for the Central Universities, No. 21609408*

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336

被引次数

1

摘要：

背景：目前对心肌梗死的急性期是否能进行适量的运动，且适量的运动是否对梗死心肌修复与再生有积极的意义，尚存在很大的争议。
目的：观察中等强度运动对心肌梗死大鼠梗死面积的影响。
方法：将48只SD大鼠随机分为雌、雄训练组和雌、雄对照组，每组12只。结扎大鼠左冠状动脉前降支建立左心室前壁梗死模型。造模24 h，训练组大鼠进行30 min/d的跑台训练，强度为20 m/min，0% grade，共持续12 d；对照组造模后正常饲养。2周后，对大鼠心肌行常规Masson’s Trichrome染色，观察梗死面积和左心室几何参数的变化。
结果与讨论：造模2周，训练组和对照组大鼠的死亡率十分接近(22% vs. 20%)。训练组(雌、雄)的心肌梗死面积明显小于对照组(P < 0.05)，梗死边缘区室壁厚度大于对照组(P < 0.05)，梗死区室壁最小厚度也大于对照组，但差异无显著性意义 (P > 0.05)。说明心肌梗死急性期即进行中等强度的跑台训练不但没有明显降低大鼠的存活率，还能有效减小缺血心肌的梗死面积、改善左心室重构，且上述作用没有性别差异。

关键词: 跑台训练, 心肌梗死, 心肌再生, 梗死面积, 重构

Abstract:

BACKGROUND: It is still controversy regarding to whether moderate training, treated in acute period of myocardial infarction (MI), plays a benefit role in regeneration of myocardial infarction.
OBJECTIVE: To investigate the effect of moderate training on infarct areas after myocardial infarction.
METHODS: Forty-eight Sprague-Dawley (SD) rats were randomly divided into four experimental groups (male and female trained group and female and male control group), In 24 hours after left anterior descending coronary artery (LAD) ligation, the trained groups were introduced to treadmill training (20 m/min, 0% grade, 30 min/d) for 12 days, while the control groups were fed normally after LAD-ligation. The infarcted size and the geometry of left ventricle (LV) of the trained and control groups were analyzed respectively under the Masson’s Trichrome staining in two-week of MI.
RESULTS AND CONCLUSION: It was found that the mortality of trained group was similar to the control group (trained group: 22% vs. control group: 20%). The infarction size of trained group was less than that of control group in two weeks after MI. The difference had statistics significance (P < 0.05). In addition, it was found that the wall thickness of border zone in left ventricle of trained group was longer than control (P < 0.05); while the thickness of infarcted myocardium in LV was greater than that of the control group. All results demonstrated that the moderate-intensity treadmill training treated in acute period of MI did not significantly reduce the survival rate of rats. On the contrary, it was able to reduce the infarcted size of MI. In addition, the moderate treadmill training was able to improve the reconstruction of LV geometry after MI. The effect has no difference on different genders.

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