中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2661-2684.doi: 10.3969/j.issn.1673-8225.2011.15.001

• 骨组织构建 bone tissue construction •    下一篇

不同强度静磁场对成骨细胞细胞骨架改建的影响

王胜国1，周  力2，陈扬熙2   

  1. 1重庆医科大学附属第二医院口腔科，重庆市  400010
    2四川大学华西口腔医学院口腔正畸科，四川省成都市  610041
  • 收稿日期:2010-11-08 修回日期:2010-12-20 出版日期:2011-04-09 发布日期:2013-11-06
  • 作者简介:王胜国☆，男，1976年生，汉族，山东省德州市人，博士，主治医师，主要从事错牙合畸形牙的矫治及其机制研究。 Wangshengguo 76@163.com

Effect of different-intensity static magnetic field on the reorganization of osteoblast cytoskeleton

Wang Sheng-guo1, Zhou Li2, Chen Yang-xi2   

  1. 1Department of Stomatology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
    2Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu  610041, Sichuan Province, China
  • Received:2010-11-08 Revised:2010-12-20 Online:2011-04-09 Published:2013-11-06
  • About author:Wang Sheng-guo☆, Doctor, Attending physician, Department of Stomatology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China wangshengguo76@ 163.com

PDF

612

被引次数

25

摘要：

背景：静磁场对成骨细胞增殖活性、细胞因子分泌及碱性磷酸酶表达等功能活性影响的机制尚不清楚。
目的：观察不同强度静磁场加载对成骨细胞骨架改建和细胞骨架蛋白表达的影响。
方法：取新生24 h内SD大鼠颅骨进行成骨细胞的培养和鉴定。采用静磁场加载装置对体外培养的成骨细胞进行0，8，50，160 mT的静磁场加载，分别于静磁场加载24，48，72 h应用BODYPY-Phaloidin进行成骨细胞骨架染色，激光扫描共聚焦显微镜和图像处理软件Image J测定细胞骨架蛋白的荧光强度。
结果与结论：8，50，160 mT静磁场加载24，48，72 h，荧光标记的成骨细胞骨架蛋白向细胞核周围集中，荧光强度增强(P < 0.05)，其中经50 mT静磁场加载的成骨细胞骨架蛋白的荧光最强。说明一定强度的静磁场可以影响成骨细胞骨架的改建和重组。

关键词: 静磁场, 成骨细胞, 细胞骨架, 细胞荧光, 骨组织工程

Abstract:

BACKGROUND: Mechanisms addressing effects of static magnetic field on osteoblast proliferation, cytokine secretion and alkaline phosphatase (ALP) activity remain poorly understood.
OBJECTIVE: To evaluate the effects of static magnetic field loading the cytoskeleton reorganization and cytoskeletal protein expression.
METHODS: The osteoblasts were harvested from skull of SD rats within 24 hours after born and cultured. Static magnetic fields with 0, 8, 50 and 160 mT were added on osteoblasts. The BODYPY-Phaloidi was used to stain cytoskeleton, and fluorescence intensity was measured by laser scanning confocal microscope and image J at 24, 48 and 72 hours after loading.
RESULTS AND CONCLUSION: At 24, 48 and 72 hours after culturing under 8, 50 and 160 mT static magnetic fields, the cytoskeleton fluorescence concentrated to the nucleus and the fluorescence intensity up-regulated (P < 0.05), especially strongest in the 50 mT static magnetic field. The static magnetic field can result in the reorganization of the osteoblast cytoskeleton.  

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